Application of macromolecular protein in antibacterial and antiviral disinfectant

A macromolecular and disinfectant technology, applied in the direction of antiviral, disinfectant, antifungal, etc., can solve the problems of lack of understanding, corruption and degradation, variability, etc., to achieve strong stability and activity, long action time, Active lasting effect

Inactive Publication Date: 2020-12-18
武汉愔紫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This vacancy is mainly due to the fact that there are very few studies on the physical and chemical stability of these RNases in the prior art, and there is a lack of sufficient understanding, coupled with people's common sense concept of protein properties, it is believed that enzyme proteins need body temperature (37°C) to perform enzyme functions and activity, the protein is unstable in the natural environment, easily denatured, spoiled and degraded, but cannot last in vitro, kill pathogenic microorganisms, and play a role in preventing diseases

Method used

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  • Application of macromolecular protein in antibacterial and antiviral disinfectant
  • Application of macromolecular protein in antibacterial and antiviral disinfectant
  • Application of macromolecular protein in antibacterial and antiviral disinfectant

Examples

Experimental program
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Effect test

Embodiment 1

[0032] An antibacterial and antiviral disinfectant of macromolecular protein, the disinfectant contains one or more recombinant human RNase enzyme family proteins.

[0033] The record entries of human RNases in the Uniprot database are shown in the table below:

[0034]

[0035]

[0036] These proteins act as enzymes and they catalyze the following reactions:

[0037]An(RNA)containing cytidine+H(2)O=an(RNA)-3'-cytidine-3'-phosphate+a5'-hydroxy-ribonucleotide-3'-(RNA)An(RNA)containing uridine+H( 2) O=an(RNA)-3'-uridine-3'-phosphate+a 5'-hydroxy-ribonucleotide-3'-(RNA)

[0038] These proteins are mainly naturally distributed outside the cells of various organs and tissues throughout the body, and are also secreted to the body surface, oral cavity, intestinal tract, and urethra through tears, saliva, mucus, and sweat. As natural immune molecules, they resist pathogenic microorganisms in the human body.

[0039] The RNases in this example are recombinant human-derived RNas...

Embodiment 2

[0046] The stability of embodiment 2 disinfectants

[0047] (1) Thermal stability of recombinant RNases

[0048] Under various temperature conditions, the activity changes of three RNases were tested. The RNase activity detection process is as follows: 40 mg of yeast tRNA is added as a substrate to 0.8 mL of reaction solution (containing 40 mM sodium phosphate, pH 7.0 and 10 mL of rhRNase2, or rhRNase3, or rhRNase7, or buffer control). Reactions were terminated by adding 40 nM lanthanum nitrate in ice-cold 3% perchloric acid at given time points. Afterwards, centrifugation, acid-soluble ribonucleotides remaining in the supernatant were quantified spectrophotometrically at 260 nM. All time points were tested in triplicate. Calculations included the following approximations: tRNA had an average molecular weight (Mr) of Mr = 28,100 (75-90 ribonucleotides / tRNA molecule 1 Mr 341 / ribonucleotides) with an absorbance at 260 nm of 1.0, corresponding to 40 mg RNA. Taking the RNase a...

Embodiment 3

[0062] The bacteriostasis of embodiment 3 disinfectant

[0063] Common clinical pathogenic bacteria and fungi were incubated in 100μl 10mM sodium phosphate buffer (pH 7.4) containing 1% tryptone soybean broth and RNase7 at different concentrations above at 37°C for 3 hours, then transferred to culture Plate overnight, count the colonies (CFU) the next day, and then convert to CFU / mL.

[0064] Test results such as Figure 6 Shown in order are Enterobacter (Escherichia coli), Staphylococcus aureus (Staphylococcus aureus), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Prupionibacterium acnes, Enterococcus faecium (Enterococcus Faecium), Candida albicans (candidaalbicans) statistical graph; As can be seen from the figure, RNase7 has an inactivation effect on all these pathogenic bacteria, and the sensitivity of various pathogenic bacteria to RNase is different, with the highest sensitivity of Enterococcus, Candida albicans (fungi ) has the lowest sensitivity. It is worth not...

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Abstract

The invention provides application of macromolecular protein in an antibacterial and antiviral disinfectant. The macromolecular protein is one or more of natural immune polypeptide and protein secreted by bacteria, fungi, animals or plants in the biological field, and the disinfectant is applied to an in-vitro environment, a body surface environment or an oral and nasal cavity environment. The macromolecular protein can stably exist in an in-vitro environment and has lasting antivirus and sterilization activity, and the macromolecular protein is one or more of recombinant human-derived RNAses.Natural immune substances in the biological field are applied to in-vitro disinfection products, so that inactivation of germs and viruses in the environment is realized, and the traditional conceptthat natural immune proteins are only used for medicines in human bodies is changed.

Description

technical field [0001] The present invention relates to the technical field of disinfection products, in particular to the application of a macromolecular protein in antibacterial and antiviral disinfectants, specifically, the use of natural immune molecule RNases in the human body for in vitro sterilization and disinfection to prevent germ infection or reduce germs in the environment Load, reduce infection. Background technique [0002] Human anti-virus and anti-bacteria rely on the immune ability of the human body, there are innate immunity and infection-acquired immunity after the vaccine. Innate immunity includes cellular immunity and humoral immunity. Innate humoral immunity consists of various anti-bacterial, fungal, and viral polypeptides, immune factors, and certain active proteins in the body. Among them are ribonucleases (RNases), which are secreted in fluids such as tears, saliva, mucus and sweat to defend against invading microorganisms. The RNase A superfamil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/50A01P1/00A61K38/46A61P31/04A61P31/10A61P31/12
CPCA61K38/465A61P31/04A61P31/10A61P31/12A01N63/50A01N63/30A01N63/14A01N63/10
Inventor 朱小湘
Owner 武汉愔紫生物科技有限公司
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