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Environmental response type paecilomyces lilacinus microsphere and preparation method thereof

A technology of Paecilomyces lilacinus and environment response, applied in the field of environment-responsive Paecilomyces lilacinus microbeads and preparation thereof, can solve problems such as inability to improve the growth environment of crops, pathogenic microorganisms, etc., so as to avoid early recovery and protect bacteria. a kind of activity, the effect of increasing gel strength

Active Publication Date: 2020-12-22
江西华威科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are mainly three kinds of biological fertilizers: liquid, powder and granule. These types of biological fertilizers can only have a short-term effect in farmland, and most of the bacteria will continue to be inactivated or lost as the storage time increases. As a result, there are fewer strains of bacteria that finally reach the roots of crops, so it is impossible to improve the growth environment of crops and effectively inhibit the growth of pathogenic microorganisms

Method used

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  • Environmental response type paecilomyces lilacinus microsphere and preparation method thereof
  • Environmental response type paecilomyces lilacinus microsphere and preparation method thereof
  • Environmental response type paecilomyces lilacinus microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: A kind of water-responsive Paecilomyces lilacinus microbeads

[0032] (1) Preparation of core material solution: Add 250g of Paecilomyces lilacinus spore powder, 600g of corn oil, and 20g of sodium citrate into a beaker, stir at room temperature for 30min, mix well, then add surfactant dodecylphenol polyoxyethylene 10g of a mixture of ether, polyoxyethylene lauryl ether and sodium lauryl sulfate (mass ratio 2:2:1), after forming an emulsifiable concentrate, add 15g of glucose, 10g of peptone, and 40g of cellulose nanofibers in sequence, and stir at room temperature for 30min. Obtain the core material solution.

[0033] (2) Preparation of wall material solution: Add 1.5g of chitosan, 10g of gelatin, 4g of polyvinyl alcohol (the degree of polymerization of polyvinyl alcohol is 500-600), and 8g of glycerin into 80mL of distilled water, heat to 95-100°C, and mix Evenly, keep warm at 95-96°C to get wall material solution.

[0034] (3) core material and wall m...

Embodiment 2

[0070] Embodiment 2 A kind of water response type Paecilomyces lilacinus microbeads

[0071] (1) Preparation of core material solution: Add 200g of Paecilomyces lilacinus spore powder, 400g of soybean oil, and 25g of sodium citrate into a beaker, stir at room temperature for 30min, and mix well, then add the surfactant octylphenol polyoxyethylene ether, After the mixture of fatty alcohol sodium sulfate 12g (mass ratio 2:1) forms an emulsifiable concentrate, add 10g of glucose, 5g of peptone, and 30g of cellulose nanofibers in sequence, and stir at room temperature for 30min to obtain a core material solution.

[0072] (2) Preparation of wall material solution: Add 1.5g of chitosan, 8g of gelatin, 3g of polyvinyl alcohol (the degree of polymerization of polyvinyl alcohol is 500-600), 6g of glycerin into 60mL distilled water, heat to 95-100°C, mix Evenly, keep warm at 95-96°C to get wall material solution.

[0073] (3) core material and wall material solution that step (1) (2) ...

Embodiment 3

[0074] Embodiment 3 A kind of water response type Paecilomyces lilacinus microbeads

[0075] (1) Prepare the core material solution: add 300g of Paecilomyces lilacinus spore powder, 500g of rapeseed oil, and 30g of sodium citrate into a beaker, stir at room temperature for 30min, mix well, then add surfactant castor oil polyoxyethylene ether, A mixture of styrylphenol polyoxyethylene ether and calcium dodecylbenzenesulfonate 12g (mass ratio 3:1:1), after forming an emulsifiable concentrate, add 12g of glucose, 8g of peptone, and 50g of cellulose nanofibers in sequence, and stir at room temperature 30min, to obtain the core material solution.

[0076] (2) Preparation of wall material solution: Add 2.0 g of chitosan, 10 g of gelatin, 5 g of polyvinyl alcohol (the degree of polymerization of polyvinyl alcohol is 500-600), and 10 g of glycerin into 100 mL of distilled water, heat to 95-100 ° C, and mix Evenly, keep warm at 95-96°C to get wall material solution.

[0077] (3) core...

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Abstract

The invention relates to an environmental response type paecilomyces lilacinus microsphere as well as a preparation method and application thereof, and belongs to the technical field of agricultural microorganism development. According to the environmental response type paecilomyces lilacinus microsphere, polyvinyl alcohol in a shell can be stimulated to be dissolved according to a moisture content in soil, so that water in the soil flows into the microsphere; cellulose nanofibers in the microsphere absorb water and expand to block a small hole in the shell, so that spores cannot flow out; andwhen spores encounter water, the spores are germinated and propagated by utilizing nutrient substances in the microsphere, protease and chitinase secreted by paecilomyces lilacinus can degrade gelatin and chitosan in shell in a propagation process, and a release effect can be achieved after a certain period of time. In a region with much rainwater or in irrigated soil, the microsphere can increase a release quantity of strains and improve the utilization efficiency of a fertilizer.

Description

Technical field: [0001] The invention relates to an environment-responsive Paecilomyces lilacinus microbead, a preparation method and application thereof, and belongs to the technical field of agricultural microorganism development. Background technique: [0002] Paecilomyces lilacinus belongs to the subphylum Deuteromycetes, Hyphospora, and Paecilomyces genus, which is an effective egg-parasitic fungus for phytopathogenic nematodes. More than 50 species have been found so far, all of which belong to entomopathogenic bacteria or nematode pathogenic bacteria. Studies have found that Paecilomyces lilacinus can not only promote the growth of various plants, but also prevent and control many plant diseases. It has many advantages such as safety, pollution-free, high efficiency, and wide host range. [0003] In recent years, climate change has been serious, and some areas have sustained large-scale rainfall, resulting in the phenomenon of precipitation increase and decrease in di...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/79
CPCC12N1/14C12N3/00A01N25/28A01P5/00B01J13/04A01N65/30A01N25/30A01N65/00B01J13/14
Inventor 邹立强吴通陈贵忠刘伟
Owner 江西华威科技有限公司
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