Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rhodosporidium paludigenum and application thereof in producing beta-carotene by taking straws as raw materials

A technology of marine red yeast and carotene, applied in microorganism-based methods, fermentation, microorganisms, etc., can solve the problems of high requirements on lighting and nutrient conditions, difficult to use dissolved oxygen, and high viscosity of fermentation broth, and achieve high efficiency and high quality. The effect of easy control of production and fermentation and fast growth rate

Active Publication Date: 2020-12-29
清华苏州环境创新研究院 +1
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Salina and Blakeslea trispora are the two main strains currently used in the production of β-carotene on the market, which have the advantages of fast proliferation and high content; however, the growth of Salina has higher requirements on light and nutritional conditions, and the algae species It is easy to degrade, and the fermentation liquid of B. trispora has high viscosity and difficult to use dissolved oxygen, which has certain limitations in large-scale production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhodosporidium paludigenum and application thereof in producing beta-carotene by taking straws as raw materials
  • Rhodosporidium paludigenum and application thereof in producing beta-carotene by taking straws as raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of fiber hydrolyzate:

[0045] 1) The raw material of wheat straw was washed and chopped, and the wheat straw was pretreated by steam explosion. The conditions of steam explosion were as follows: the temperature of saturated steam was 230°C, the pressure was 0.2 Mpa, and the holding time was 2 min;

[0046] 2) The steam-exploded wheat straw is then treated with sodium hydroxide and sodium sulfite, the weight of sodium hydroxide: dry weight of wheat straw raw material is 1:4, the weight ratio of sodium sulfite: sodium hydroxide is 1:5, and the treatment conditions are: The reaction temperature was 150 °C and the time was 1 h. After fully removing the lignin, extract the wheat straw fiber, dehydrate and fully wash away the residual lye;

[0047] 3) Soak straw fibers in acetic acid-sodium acetate (or similar buffer pair with buffering effect) buffer solution, add cellulase, and hydrolyze the fiber slurry into a high-concentration sugar solution; the hydrol...

Embodiment 2

[0055] (1) Preparation of fiber hydrolyzate:

[0056] 1) The raw material of rice straw was washed and chopped, and the rice straw was pretreated by steam explosion. The conditions of steam explosion were as follows: the temperature of saturated steam was 210°C, the pressure was 1.2 Mpa, and the holding time was 8 minutes;

[0057] 2) The steam-exploded wheat straw is then treated with sodium hydroxide and sodium sulfite, the weight of sodium hydroxide: the absolute dry weight of rice straw raw material is 1:6, the weight ratio of sodium sulfite: sodium hydroxide is 1:3, and the treatment conditions are: The reaction temperature was 160 °C and the time was 1 h. After fully removing the lignin, extract the wheat straw fiber, dehydrate and fully wash away the residual lye;

[0058] 3) Soak straw fibers in acetic acid-sodium acetate (or similar buffer pair with buffering effect) buffer solution, add cellulase, and hydrolyze the fiber slurry into a high-concentration sugar soluti...

Embodiment 3

[0069] (1) Preparation of fiber hydrolyzate:

[0070] 1) The corn stalks were washed and chopped, and the corn stalks were pretreated by steam explosion. The steam explosion conditions were as follows: the saturated steam temperature was 250 °C, the pressure was 0.6 Mpa, and the holding time was 5 min;

[0071] 2) The steam-exploded corn stalks are then treated with sodium hydroxide and sodium sulfite. The weight of sodium hydroxide: the absolute dry weight of corn stalks is 1:5, and the weight ratio of sodium sulfite: sodium hydroxide is 1:5. The treatment conditions are: The reaction temperature was 150 °C and the time was 2 h. After fully removing the lignin, extract the corn stalk fiber, dehydrate and fully wash away the residual lye;

[0072] 3) Soak straw fiber in acetic acid-sodium acetate (or similar buffer pair with buffering effect) buffer solution, add cellulase, and hydrolyze the fiber slurry into a high-concentration sugar solution; the hydrolysis conditions are ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
tolerance concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses rhodosporidium paludigenum and a method for producing beta-carotene (BC) by using the rhodosporidium paludigenum by taking straws as a raw material. Agricultural straw is a renewable organic resource which is wide in distribution, large in number and difficult to treat, straw fibers obtained after steam explosion and alkaline pretreatment are subjected to enzyme hydrolysisto obtain hydrolysate, extra nutrition is added after the hydrolysate is decolored and used for culturing rhodosporidium paludigenum, and beta-carotene is directly extracted from obtained thalli, andthe limitation of traditional beta-carotene large-scale production is avoided, and meanwhile the recycling value of straws is improved. The biomass of the rhodosporidium paludigenum can reach 59 g / L,the yield of glucose thallus is 0.5-0.6, and the yield of beta-carotene is 550-650 mu g / g dry thallus.

Description

technical field [0001] The invention belongs to the field of microbial agent products, and in particular relates to a marine red yeast and its application in the production of beta-carotene by using straw as a raw material. Background technique [0002] β-carotene (β-carotene, BC) is a natural pigment ubiquitously present in plants, the color is yellow to orange-red, and it is one of the carotenoids. β-carotene is a fat-soluble vitamin, which is the main source of synthetic vitamin A for humans and animals. It has strong antioxidant properties, good anti-cancer effects, regulatory effects on the immune system and coloring functions. It is widely used in food and feed , pharmaceutical and cosmetic industries. The sources of β-carotene are mainly divided into plant extraction, chemical synthesis and microbial fermentation. Plant extraction is limited by a series of conditions such as raw materials, climate, and place of origin, making it difficult to produce in large quantit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12N1/16C12R1/645
CPCC12N1/16C12P23/00C12P2201/00C12P2203/00C12R2001/645C12N1/145
Inventor 张天元李振
Owner 清华苏州环境创新研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com