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Cellulose degradation strain with sewage ammonia nitrogen degradation capacity and application thereof

A strain, Bacillus technology, applied in the field of microorganisms, can solve the problem of low enzyme activity

Active Publication Date: 2021-01-05
SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet the contriver finds, the bacillus bacterial strain that the bacillus strain that the selected bred of report at present has low enzymatic activity, and the bacterial species of high-efficiency enzyme production is less, therefore, still need to search for new high-yield cellulase bacillus bacterial strain

Method used

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  • Cellulose degradation strain with sewage ammonia nitrogen degradation capacity and application thereof
  • Cellulose degradation strain with sewage ammonia nitrogen degradation capacity and application thereof
  • Cellulose degradation strain with sewage ammonia nitrogen degradation capacity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment one, the screening of strain

[0070] 1. Materials and methods

[0071] 1.1 Samples: 6 samples of decayed straw materials from different farmlands were used as samples.

[0072] 1.2 Screening medium:

[0073] The strain enrichment medium is as follows: potassium dihydrogen phosphate, sodium chloride, ferric chloride, magnesium sulfate, sodium nitrate and calcium chloride are added to water, after mixing evenly, wheat straw cut into about 2 cm length is added as the only carbon The source is available, its pH is 7.0. Each liter of water contains 1.0 g of potassium dihydrogen phosphate, 0.1 g of sodium chloride, 0.01 g of ferric chloride, 0.3 g of magnesium sulfate, 2.5 g of sodium nitrate, and 0.1 g of calcium chloride.

[0074] The preparation method of the CMC-Na Congo red culture medium is as follows: sodium carboxymethylcellulose, potassium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, sodium chloride and agar are added into water, and mixed...

Embodiment 2

[0110] Embodiment two, identification and safety test of bacterial classification

[0111] 1. Materials and methods

[0112] 1.1 Bacterial strains: After primary screening and re-screening of microorganisms isolated from spoiled straw and other samples, a total of 4 strains with high cellulose degradation performance were screened out, and the numbers are NBL-B11010, NBL-B12006, and NBL-B12010 respectively. and NBL-B11026.

[0113] 1.2 Morphological identification and molecular identification of bacteria: Unless otherwise specified, general morphological and molecular tests were carried out referring to "Bergey's Bacterial Identification Manual" (9th edition) and "Microbiology Experimental Manual".

[0114] 1.3 Safety test: The phytotoxicity test of the bacteria was carried out using "Pearl" tomato seeds and "Crystal" lettuce seeds. The seeds were soaked in 2% concentration of single bacterial solution, mixed bacterial solution (1:1:1) and distilled water (control group) for...

Embodiment 3

[0124]Embodiment 3, Bacillus velezensis strain (Bacillus velezensis strain) NBL-B11010 produces cellulase condition optimization

[0125] 1. Materials and methods

[0126] 1.1 Strains: NBL-B11010

[0127] 1.2 Method:

[0128] The effects of rotation speed, temperature, inoculation volume of bacterial solution, initial pH, nitrogen source and carbon source on cellulase production of the strain were investigated by single factor experiment, and the optimal fermentation conditions were determined.

[0129] 2 Test content and results

[0130] 2.1 Shaker speed

[0131] During the strain cultivation process, the speed of the shaking table will affect the contact between the strain and nutrients, and also affect the contact between the strain and oxygen. Too high or too low rotation speed will affect its cellulase production effect. With the increase of the culture rotation speed, the cellulase-producing ability of the bacteria first increased and then decreased. When the cultur...

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Abstract

The invention provides a cellulose degradation strain with sewage ammonia nitrogen degradation capacity, and belongs to the technical field of microorganisms. The strain is Bacillus Velezensis strainNBL-B11010. The strain is preserved in China Center for Type Culture Collection on August 31, 2020, and the preservation number of the strain is CCTCC NO: M 2020458. The strain is obtained from a farmland rotten straw material through screening, purification, identification and other technologies, safety tests and fermentation conditions of the strain are studied, straw degradation efficiency andsewage ammonia nitrogen degradation efficiency are measured, it is shown that the strain is a beneficial strain with efficient cellulose degradation and sewage ammonia nitrogen degradation capacity, and has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a cellulose-degrading bacterial strain capable of degrading ammonia nitrogen in sewage and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The output of crop straw along the Huaihe River is abundant, but the utilization rate of straw returning to the field is not high. This is because the decomposition of returning to the field is slow in the natural state, which will affect the cultivation and crop growth. Shajiang black soil is the main soil in the Huaihe River area, and it is one of the typical low-yielding soil types. Poor soil nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20A01N63/22A01P21/00C02F3/34C02F101/16C12R1/07C12R1/125C12R1/10
CPCA01N63/22C02F3/34C02F2101/16C12N1/20C05F17/20C12N1/205C12R2001/07Y02W30/40
Inventor 胡著然王丽荣郭静王晓博朱胜男张国珍刘新玲吴敏樊梅娜张文娟申小冉董雪雁
Owner SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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