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Method for detecting aflatoxin based on iron ion probe-nanogold/glassy carbon electrode modified electrode

A kind of technology of aflatoxin and glassy carbon electrode, which is applied in the direction of measuring device, material electrochemical variable, material analysis by electromagnetic means, etc., can solve the problem that the daily detection of aflatoxin has not been well solved, the toxin cannot be detected in real time, Aflatoxin detection sensitivity is not high, to achieve the effect of convenient operation, cost reduction and high sensitivity

Active Publication Date: 2021-01-05
SHAANXI UNIV OF SCI & TECH
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  • Description
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  • Application Information

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Problems solved by technology

However, the detection sensitivity of aflatoxins is not high at present, and the toxins in human tissue fluid and the environment cannot be detected immediately. Therefore, the daily detection of aflatoxins in the human body has not been well solved.

Method used

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  • Method for detecting aflatoxin based on iron ion probe-nanogold/glassy carbon electrode modified electrode
  • Method for detecting aflatoxin based on iron ion probe-nanogold/glassy carbon electrode modified electrode
  • Method for detecting aflatoxin based on iron ion probe-nanogold/glassy carbon electrode modified electrode

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preparation example Construction

[0036] Wherein, in the above-mentioned detection method of aflatoxin based on the iron ion probe-nano gold / glassy carbon electrode modified electrode, the preparation method of the used nano gold / glassy carbon electrode modified electrode comprises the following steps:

[0037] a) Electrode pretreatment

[0038] A three-electrode system is adopted, including a working electrode (glassy carbon electrode), a counter electrode (platinum wire electrode) and a reference electrode (Ag / Ag / Cl). The three-electrode system consists of two loops, one loop consisting of the working electrode and the reference electrode, which is used to measure the potential of the electrode, and the potential of the reference electrode is known. The working electrode and the counter electrode form another loop to measure the current, that is, "three electrodes and two loops".

[0039] First, rinse the surface of the glassy carbon electrode with prepared distilled water three times repeatedly, and then p...

Embodiment 1

[0052] The invention discloses a method for preparing an electrochemical sensor based on an iron ion probe, which specifically includes the following steps:

[0053] a) Electrode pretreatment

[0054] Firstly, the surface of the glassy carbon electrode was rinsed repeatedly with prepared distilled water, and then the glassy carbon electrode was polished with polishing powder containing aluminum oxide particles on a wet polishing flannelette. The glassy carbon counter electrode and reference electrode were rinsed clean for later use.

[0055] b) Preparation of chloroauric acid solution

[0056] Chlorauric acid solution from HAuCl 4 4H 2 O and KCl were prepared according to the molar concentration ratio (final concentration ratio in solution) of 1:20. First accurately weigh the required HAuCl 4 4H 2 O, ultrasonically dissolve in ultrapure water, add KCl after fully dissolved, continue to ultrasonically dissolve until fully dissolved, and finally obtain a chloroauric acid s...

Embodiment 2

[0067] The electrochemical sensor based on the iron ion probe was prepared by the preparation method described in Example 1, and the above electrochemical sensor based on the iron ion probe was used to detect AFM in milk 1 Application of content:

[0068] 1) Prepare aflatoxin standard solution, prepare iron ion (Fe 3+ ) solution

[0069] aflatoxin M 1 (AFM 1 ) standard substance, dissolved in methanol to obtain toxin stock solution;

[0070] Fe 3+ Solution (concentration: 5mmol / L): Prepared with water-soluble iron salts such as potassium ferricyanide, ferric chloride or ferric sulfate.

[0071] Toxin stock solution with Fe 3+ The mixing volume ratio of the solutions is 1:3.

[0072] 2) AFM 1 Standard solution detection

[0073] With the conditions controlled when modifying the electrode in Example 1 as optimal conditions, differential pulse voltammetry (DPV) was used to analyze the different concentrations of AFM 1 To scan, the concentration gradient of the aflatoxin...

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Abstract

The invention discloses a method for detecting aflatoxin based on iron ion probe-nanogold / glassy carbon electrode modified electrode, and belongs to the technical field of biology and new medicine. Byutilizing the advantages of large specific surface area, strong adsorption capacity, high electron density, dielectric property, catalytic action and the like of the nanogold, the detection activityand stability are enhanced; secondly, the modified electrode is prepared in an electropolymerization mode, and the process is simple and convenient; finally, Fe<3+> is used as a probe, the specificityof the Fe<3+> combined with aflatoxin enables a response signal to generate obvious fluctuation, high detection sensitivity can be achieved, and a single molecule detection level is achieved. The method has the advantages of low price, simple operation, high recovery rate, low detection limit, fast response and the like while ensuring high sensitivity, stability and practicability, and can realize real-time monitoring of toxin terminal level and effective detection of in-vivo small molecule toxin pollution by detecting toxins and toxin metabolites in blood, urine and milk.

Description

technical field [0001] The invention belongs to the technical field of biology and new medicine, and relates to a method for detecting aflatoxin based on an iron ion probe-nano gold / glassy carbon electrode modified electrode. Background technique [0002] For the detection of aflatoxins, existing traditional technical methods include thin-layer chromatography, high-performance liquid chromatography, liquid chromatography-mass spectrometry, immunochromatography, and enzyme-linked immunosorbent assay. The above detection methods are commonly used methods in laboratories, among which the application of large-scale instrument detection requires high operating space, complicated operation, cumbersome sample pretreatment process and difficulty in real-time monitoring, and the detection results are easily affected by operators and external environments. Immunological detection methods have strong specificity, but the existing products on the market are expensive, cannot achieve lar...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/301G01N27/308G01N27/3278G01N27/48
Inventor 胡梁斌徐丹张苗李晓波莫海珍李红波黄敏敏
Owner SHAANXI UNIV OF SCI & TECH
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