Protein-producing strain and application thereof

A protein and mycelium technology, applied in the direction of fungi, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effect of complete types, rich types, and wide application value

Active Publication Date: 2021-01-15
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the efficient and direct production of mycelial protein

Method used

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  • Protein-producing strain and application thereof
  • Protein-producing strain and application thereof
  • Protein-producing strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018]Example 1. FusariumFusarium venenatum Acquisition of TB01

[0019]The rhizosphere soil of wheat land was collected in Tianjin Binhai New Area in July 2020.

[0020]Separation process: Mix the rhizosphere soil sample, weigh 5 g, put it into a triangular flask containing 95 mL of sterile water and 10 glass beads, and shake at 30°C and 180 rpm for 30 min. Take 1 mL of soil suspension for 10-1-10-7 Serial concentration gradient dilution, then take 10-5, 10-6, 10-7Three dilutions were spread on a synthetic low-nutrient SNA medium plate, and cultured upside down at 28°C for 2 days.

[0021]Purification process: Purification is carried out by step-by-step transplantation of mycelial ends. After the colony is formed on the plate, pick the hyphae at the edge of a single colony on the PDA medium plate and continue to culture at a constant temperature of 28°C until a pure colony is obtained, which will be stored at 4°C.

[0022]Re-screening process: connect 2 loops of the obtained colonies with an i...

Example Embodiment

[0026]Example 2. Morphological characteristics of strain TB01

[0027]The strain TB01 was inoculated into PSA medium and kept at 24-25°C for 4 days. According to the growth rate, describe the shape of the hypha, the color of the colony, the number and shape of the small and large conidia, the spore-forming cells, the shape and presence of chlamydospores, and the type of fruiting body, etc., to identify the strain.

[0028]Cultivation traits: 4 d colony diameter is 3.8 cm, the hyphae on PSA medium are initially flocculent, and then become fluffy to powdery, white to pink, the surface of the substrate is white, and the substrate does not change color;

[0029]Morphological characteristics: large conidia are relatively uniform in size, thick and fat, relatively uniform in the middle, wedge-shaped top cells, basal cells without heels, 3-4 partitions, mostly 4-6 partitions. Measurement: 28. 32-42. 50 μm×4. 63-4. 54 μm; small conidia are absent or very few; no chlamydospores; spores are produced b...

Example Embodiment

[0031]Example 3. ITS sequence analysis of strain TB01

[0032]The strain TB01 was inoculated into PDA medium, cultured at 30℃, 180 rpm shaker for 24 h, collected the bacteria, extracted the total DNA, and then used it as a template, using the following universal primers for the eukaryotic ITS gene sequence as usual The method for PCR amplification of ITS gene sequence: FP1: 5'-AGTAAAAGTCGTAACAAGGT-3' and FP2: 5'-TTCACTCGCCGTTACTAGGG-3'.

[0033]After the amplified products were separated by 1% agarose gel electrophoresis, they were recovered using a gel recovery kit and handed over to Beijing Kinco Biotechnology Co., Ltd. for sequencing. The sequencing results showed that the ITS gene sequence of this strain is shown in SEQ ID No. 1. Align it with the sequence in the GenBank database, and use MEGA 7.0 software to perform multiple sequence homology analysis, and construct a phylogenetic tree, such asimage 3Shown.

[0034]According to morphological characteristics and TIS sequence analysis, th...

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Abstract

The invention relates to the technical field of microorganisms and foods, in particular to a protein-producing strain and an application thereof. Substrates available for the strain are very rich, andindustrial wastewater, agricultural and sideline products and other substrate raw materials which are wide in source and low in price can be fully utilized as a carbon source or a nitrogen source. Mycelium obtained through fermentation is very high in protein content and complete in amino acid varieties, and has wide application value.

Description

technical field [0001] The invention relates to the field of food technology, in particular to a mycelium protein-producing Fusarium and its application in the production of mycelium protein. Background technique [0002] In the late 1960s, the British Ranks Hovis McDougall (RHM) food company pioneered the research and development of mycoproteins. After extensive global search, Fusarium was finally found in the soil near the wheat field in the Marlow area of ​​the United Kingdom. As an adequate and effective source of protein. Mycelium protein products are rich in protein, generally 40% to 80%, with a complete range of amino acids and appropriate proportions, including various amino acids required by animals, especially the high content of lysine, tryptophan, threonine It is also rich in acid and isoleucine, and contains the limiting amino acids necessary for the growth of livestock and poultry, which can ensure the growth needs of livestock and poultry to the greatest exte...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P21/00C12R1/77
CPCC07K14/37C12N1/14C12N1/145C12R2001/77
Inventor 马延和李德茂齐显尼曾艳王钦宏孙媛霞
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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