Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Protein-producing strain and application thereof

A protein and mycelium technology, applied in the direction of fungi, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effect of complete types, rich types, and wide application value

Active Publication Date: 2021-01-15
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the efficient and direct production of mycelial protein by microbial fermentation still needs further development and research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein-producing strain and application thereof
  • Protein-producing strain and application thereof
  • Protein-producing strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, Fusarium Fusarium venenatum Acquisition of TB01

[0019] In July 2020, the rhizosphere soil of wheat land was collected in Binhai New Area, Tianjin.

[0020] Separation process: Mix the rhizosphere soil sample evenly, weigh 5 g, put it into a Erlenmeyer flask filled with 95 mL of sterile water and 10 glass beads, shake it at 30°C and 180 rpm for 30 min. Take 1 mL of soil suspension for 10 -1 -10 -7 Serial concentration gradient dilution, and then take 10 -5 、10 -6 、10 -7 The three dilutions were spread on the plate of synthetic low-nutrient SNA medium, and cultured upside down at 28°C for 2 days.

[0021] Purification process: Purification is carried out by the step-by-step grafting method of mycelium end. After the colony is formed on the plate, pick the hyphae at the edge of the single colony and place it on the PDA medium plate, and continue to cultivate at a constant temperature of 28°C until pure colonies are obtained, and store the obtained c...

Embodiment 2

[0026] Embodiment 2, the morphological characteristics of bacterial strain TB01

[0027] The strain TB01 was inoculated into PSA medium and incubated at 24-25°C for 4 days. According to the growth rate, describe the shape of mycelium, the color of the colony, the number and shape of small conidia and macroconidia, sporogenous cells, the shape and presence of chlamydospores, and the type of fruiting body, etc., to identify the strain.

[0028] Culture traits: The colony diameter is 3.8 cm at 4 days, the mycelia on the PSA medium are initially flocculent, and then become fluffy to powdery, white to pink, the surface of the substrate is white, and the substrate does not change color;

[0029] Morphological characteristics: Large conidia are relatively uniform in size, thick and fat, relatively uniform in the middle, wedge-shaped at the top, basal cells without heels, separated by 3-4, and mostly separated by 4-6. Dimensions: 28. 32-42. 50 μm×4. 63-4. 54 μm; microconidia absent o...

Embodiment 3

[0031] Embodiment 3, the ITS sequence analysis of bacterial strain TB01

[0032]Inoculate strain TB01 into PDA medium, culture at 30°C, 180 rpm shaker for 24 h, collect the bacteria, extract the total DNA, then use it as a template, use the following general primers of eukaryotic ITS gene sequence according to the routine PCR amplification of the ITS gene sequence was performed using the following method: FP1: 5'- AGTAAAAGTCGTAACAAGGT-3' and FP2: 5'-TTCACTCGCCGTTACTAGGG-3'.

[0033] After the amplified products were separated by 1% agarose gel electrophoresis, they were recovered with a gel recovery kit and submitted to Beijing Qingke Biotechnology Co., Ltd. for sequencing. Sequencing results showed that the strain's ITS gene sequence is shown in SEQ ID No.1. Compare it with the sequence in the GenBank database, and use MEGA 7.0 software to perform multiple sequence homology analysis, and construct a phylogenetic tree, such as image 3 shown.

[0034] According to morpholog...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of microorganisms and foods, in particular to a protein-producing strain and an application thereof. Substrates available for the strain are very rich, andindustrial wastewater, agricultural and sideline products and other substrate raw materials which are wide in source and low in price can be fully utilized as a carbon source or a nitrogen source. Mycelium obtained through fermentation is very high in protein content and complete in amino acid varieties, and has wide application value.

Description

technical field [0001] The invention relates to the field of food technology, in particular to a mycelium protein-producing Fusarium and its application in the production of mycelium protein. Background technique [0002] In the late 1960s, the British Ranks Hovis McDougall (RHM) food company pioneered the research and development of mycoproteins. After extensive global search, Fusarium was finally found in the soil near the wheat field in the Marlow area of ​​the United Kingdom. As an adequate and effective source of protein. Mycelium protein products are rich in protein, generally 40% to 80%, with a complete range of amino acids and appropriate proportions, including various amino acids required by animals, especially the high content of lysine, tryptophan, threonine It is also rich in acid and isoleucine, and contains the limiting amino acids necessary for the growth of livestock and poultry, which can ensure the growth needs of livestock and poultry to the greatest exte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14C12P21/00C12R1/77
CPCC07K14/37C12N1/14C12N1/145C12R2001/77
Inventor 马延和李德茂齐显尼曾艳王钦宏孙媛霞
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com