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Method for improving high-polymerization-degree epsilon-polylysine in product

A high-degree-of-polymerization, poly-lysine technology, applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., which can solve the problem of surface area differential polarization, reduced agent permeation flux, and reduced membrane flux and other problems, to achieve the effect of no phase change, improved antibacterial activity, and simple process

Inactive Publication Date: 2021-01-19
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the operation of ultrafiltration membrane technology is simple, the biggest disadvantage of membrane separation technology is membrane fouling. Since the separation process ends in continuous interception of large particles, the surface area of ​​the adjacent membrane is increased by the concentration of the dissolved amount, resulting in poor polarization.
Under the effect of concentration difference, the back diffusion of solute will produce fluid resistance and local osmotic pressure increase, which eventually leads to the decrease of agent permeation flux.
If the concentration polarization is severe, it may even lead to solute precipitation and the formation of a resistive layer on the membrane surface, eventually leading to a significant decrease in membrane flux

Method used

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  • Method for improving high-polymerization-degree epsilon-polylysine in product
  • Method for improving high-polymerization-degree epsilon-polylysine in product
  • Method for improving high-polymerization-degree epsilon-polylysine in product

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Experimental program
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Effect test

Embodiment 1

[0041] The preparation of embodiment 1ε-polylysine crude product

[0042] Using S.albulus M-Z18 as the production strain of polylysine, according to the literature (Pan L, Chen X S, LiuM M, et al. Efficient production ofε-poly-L-lysine from glucose by two-stagefermentation using pH shock strategy [J]. Process Biochemistry, 2017, 63, 8-15.) method for fed-batch fermentation. Ferment to 168h and collect fermented liquid; Get fermented liquid centrifugation (rotating speed 8000rpm, time 30min), remove bacterium and other solid matter; Then carry out adsorption (bed volume 60mL by cation exchange resin D004 (Jiangsu Su Qing Water Treatment Engineering Group Co., Ltd.) , the loading liquid pH 8.5, the loading ε-PL concentration 35g / L, the loading flow rate 1BV / h), remove pigment and foreign protein; then remove salt ions by nanofiltration, using tangential flow nanofiltration system Millipore (USA Millipore Company), the molecular weight cut-off of membrane is 1KDa, and the feed l...

Embodiment 2

[0045] A method for improving high degree of polymerization ε-polylysine in products, comprising the steps of:

[0046] The epsilon-polylysine crude product obtained in Example 1 is prepared into an initial feed liquid of 20g / L, and its pH is controlled to be 6, and the temperature of the feed liquid is 25°C, and a plate membrane (PES membrane) with a molecular weight cut-off of 3000Da is used to carry out Ultrafiltration, the membrane pressure is 0.25MPa. The operation mode is intermittent variable volume diafiltration. Specifically, when the feed liquid is concentrated to 2 / 5 of the volume of the feed liquid, add water to the original volume, and so on until the high degree of polymerization ε-PL no longer appears in the permeate; Obtain ε-polylysine product.

[0047] The retentate and permeate chromatograms are as figure 2 As shown, the retentate and permeate are collected and concentrated to control the concentration at about 10g / L. from figure 2 It can be seen that ...

Embodiment 3

[0049] Adjust the molecular weight of the plate membrane as shown in Table 1, prepare the ε-polylysine crude product obtained in Example 1 into an initial feed liquid of 10 g / L, control its pH to 9, and conduct ultrafiltration at a feed liquid temperature of 25°C , the pressure across the membrane is 0.25MPa; when the feed liquid is concentrated to 2 / 5 of the volume of the raw material liquid, that is, the volume of the intercepted liquid is concentrated to 2 / 5 of the volume of the raw material liquid, the ultrafiltration is stopped, and the intercepted liquid and permeate are collected for detection.

[0050] The cut-off liquid that embodiment 3 obtains and see through liquid are carried out performance test, and test result is as follows table 1 and image 3 Shown:

[0051] The test result of table 1 embodiment 3

[0052]

[0053] It can be seen from Table 1 that the comprehensive performance of the plate membrane with a molecular weight cut off of 3000 is the best, and ...

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Abstract

The invention discloses a method for improving high-polymerization-degree epsilon polylysine in a product, and belongs to the technical field of fermented food industry. The method for improving the content of the high-polymerization-degree epsilon PL in the epsilon PL product comprises the following step: carrying out ultrafiltration treatment on an epsilon polylysine crude product, wherein the pH value of feed liquid is controlled to be 5.5-6.5 in the ultrafiltration treatment, the initial feeding concentration is 10-30 g / L, the pressure is 0.2-0.25 MPa, and a membrane adopted in the ultrafiltration treatment is a PES ultrafiltration membrane with the molecular weight cutoff of 1000-3000Da. According to the method disclosed by the invention, the proportion of the epsilon-polylysine withhigh polymerization degree in the product is increased, after ultrafiltration, the epsilon-polylysine with high polymerization degree in the product is increased by 8.4% or more than 8.4% compared with the original product, and the antibacterial activity on gram-positive bacteria and gram-negative bacteria is improved.

Description

technical field [0001] The invention relates to a method for increasing high degree of polymerization ε-polylysine in products, belonging to the technical field of fermented food industry. Background technique [0002] The impact of chemical additives on human health has been highly concerned by people, while biological additives are highly praised by people for their safety, effectiveness, and non-toxic side effects on the human body. ε-polylysine (ε-poly-L-lysine, referred to as ε-PL) is a homotype amino acid polymer formed by connecting multiple lysine residues through α-carboxyl and ε-amino groups. Most of them are 5-35, and the molecular weight range is generally 780-4600Da. It has a significant inhibitory effect on Gram-positive bacteria, Gram-negative bacteria, yeast and mold in acidic, neutral and slightly alkaline environments. Therefore, ε-PL is widely used as a green and safe biological food preservative. [0003] The antibacterial performance of ε-PL is affecte...

Claims

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Application Information

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IPC IPC(8): B01D61/14C12P13/02C12R1/465
CPCB01D61/145C12P13/02
Inventor 张建华陈旭升赵星宇张宏建
Owner JIANGNAN UNIV
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