Nucleic acid detector and nucleic acid detection method

Abstract

The invention belongs to the technical field of biological detection, and particularly discloses a nucleic acid detector and a nucleic acid detection method. The nucleic acid detector comprises a mounting rack, a reagent plate, a cover plate assembly, a heat treatment unit, a liquid moving device, an oscillating unit and a magnetic attraction assembly, wherein a nucleic acid amplification tube, ahybridization tank, a reaction tube and a reagent tube for storing an extraction reagent are arranged on the reagent plate; the cover plate assembly can move towards or away from the nucleic acid amplification tube so as to close or open a tube opening of the nucleic acid amplification tube; the heat treatment unit is used for heating or cooling the liquid on the reagent plate; the liquid moving device is used for sucking and transferring the liquid on the reagent plate; the oscillating unit is used for driving the reagent plate to oscillate back and forth in a horizontal direction; and the magnetic attraction assembly is used for carrying out magnetic attraction and magnetic release on magnetic beads in a reaction liquid on the reagent plate. The nucleic acid detection method adopts the nucleic acid detector for nucleic acid detection. According to the nucleic acid detector and the nucleic acid detection method provided by the invention, the nucleic acid detection efficiency can be improved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a nucleic acid detector and a nucleic acid detection method. Background technique [0002] Nucleic acid hybridization technology is to immobilize specific probes on solid-phase materials, and then hybridize the specific amplification products generated by nucleic acid amplification reactions, so that the samples to be tested will be mixed with probes with homologous sequences. Combining, since the sample to be tested has biotin or fluorescein or digoxin and other markers, the probe points combined with the sample to be tested have biotin or fluorescein or digoxin and other markers, and then The hybridization signal can be displayed by corresponding chemiluminescence reaction or fluorescence excitation. This detection reaction can detect a variety of target sequences in one hybridization reaction, and has the characteristics of quickness, simplicity, high sensitivity...

AI Technical Summary

Problems solved by technology

[0005] 1. The entire nucleic acid detection process requires highly qualified professional experimenters to follow up the whole process, which increases the cost of manpower and the possibility of human errors; at the same time, when dealing with possible medium and high infectious samples, it is harmful to the life safety of the experimenters and the entire experiment. The environment poses a biosecurity threat;

[0006] 2. The entire nucleic acid detection process is carried out in stages, which requires manual intervention, which increases the uncontrollability and uncertainty of the test. At the same time, the test time is long, and the detection efficiency is low or restricted;

[0007] 3. The entire nucleic acid detection process requires more than 10 types of various instruments and equipment such as medium and high-speed centrifuges, water baths, pipettes, nucleic acid extraction and purification instruments, reagent packaging instruments, nucleic acid amplification instruments, hybridization instruments, and scanners. There are as many as 15 kinds of supporting consumables; at the same time, in order to meet the testing requirements, at least 30 different solutions need to be equipped, and the equipment and preparation of the entire reagent consumables and instruments are extremely complicated;

[0008] 4. The entire nucleic acid detection process requires manual transfer of samples, various intermediate solutions, etc., and the operating environment is relatively open, which increases the risk of mutual contamination between samples and solutions, which leads to the implementation of the entire nucleic acid detection. Environmental requirements Extremely high; the consumables of the equipment equipped at the same time are expensive, not suitable for large-scale promotion and application

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