A kind of asiaticoside-chitosan-sodium alginate microsphere, preparation method and application thereof

A technology of asiaticoside and sodium alginate, which is applied in the field of biomedical materials, can solve the problems of low bioavailability and toxicity, and achieve the effects of good biocompatibility, high encapsulation rate and good sustained release effect

Active Publication Date: 2022-02-25
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the low bioavailability of asiaticoside and the deficiency of the preparation method of microspheres, that is, the toxicity of microspheres caused by the use of toxic cross-linking agents such as glutaraldehyde, and provides an asiaticoside-chitosan- Preparation method of sodium alginate microspheres

Method used

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  • A kind of asiaticoside-chitosan-sodium alginate microsphere, preparation method and application thereof
  • A kind of asiaticoside-chitosan-sodium alginate microsphere, preparation method and application thereof
  • A kind of asiaticoside-chitosan-sodium alginate microsphere, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039](a) taking 1g chitosan and adding to 100mL volume fraction is 2% glacial acetic acid solution to dissolve chitosan, preparation-chitosan acetic acid solution; the asiaticoside of 0.6g is dissolved with dehydrated alcohol to obtain product Madecassoside solution; mix the above two solutions, stir well, and add 2g of anhydrous calcium chloride to obtain the asiaticoside-chitosan acetic acid solution containing calcium chloride; use pure water Dissolve 1g of sodium alginate to obtain an aqueous solution of sodium alginate;

[0040] (b) slowly and evenly drop the prepared sodium alginate solution into the oil phase, and stir at 800rpm for 30min to form colostrum;

[0041] (c) slowly and evenly drop the asiaticoside-chitosan acetic acid solution containing calcium chloride into the colostrum, stir at a high speed of 1000 rpm, and fully emulsify for 1 hour;

[0042] (d) Slowly and evenly add 1ml of glyceraldehyde solution dropwise, after cross-linking and solidification for 1...

Embodiment 2

[0044] (a) Weighing 1g chitosan and adding it to 100mL volume fraction of 1% glacial acetic acid solution dissolves chitosan to prepare chitosan acetic acid solution; 0.4g asiaticoside is dissolved with absolute ethanol to obtain snow asiatica Grass glycoside solution; mix the above two solutions, stir evenly, until the ethanol is volatilized, add 2g of anhydrous calcium chloride to obtain the asiaticoside-chitosan acetic acid solution containing calcium chloride; dissolve with pure water 1g of sodium alginate to obtain an aqueous solution of sodium alginate;

[0045] (b) slowly and evenly drop the prepared sodium alginate solution into the oil phase, and stir at 500rpm for 40min to form colostrum;

[0046] (c) slowly and evenly drop the asiaticoside-chitosan acetic acid solution containing calcium chloride into the colostrum, stir at a high speed of 3000 rpm, and fully emulsify for 1 hour;

[0047] (d) Slowly and evenly add 1ml of glyceraldehyde solution dropwise, after cros...

Embodiment 3

[0049] (a) Weighing 1g chitosan and adding to 100mL volume fraction of 3% glacial acetic acid solution dissolves chitosan to prepare chitosan acetic acid solution; 0.6g asiaticoside is dissolved with absolute ethanol to obtain snow asiatica Grass glycoside solution; mix the above two solutions, stir evenly, until the ethanol is volatilized, add 2g of anhydrous calcium chloride to obtain the asiaticoside-chitosan acetic acid solution containing calcium chloride; dissolve with pure water The sodium alginate of 1.5g makes sodium alginate aqueous solution;

[0050] (b) slowly and evenly drop the prepared sodium alginate solution into the oil phase, and stir at 700rpm for 20min to form colostrum;

[0051] (c) slowly and evenly drop the asiaticoside-chitosan acetic acid solution containing calcium chloride into the colostrum, stir at a high speed of 3000 rpm, and fully emulsify for 1 hour;

[0052] (d) Slowly and evenly drop 2ml of glyceraldehyde solution, after cross-linking and s...

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Abstract

The invention discloses asiaticoside-chitosan-sodium alginate microspheres, a preparation method and application thereof, and belongs to the technical field of biomedical materials. The microspheres are prepared by an emulsification cross-linking method and finally obtained by freeze-drying. The preparation method comprises the following steps: 1) preparing sodium alginate solution and asiaticoside-chitosan acetic acid solution respectively; 2) slowly and uniformly adding sodium alginate solution to the oil phase, stirring at high speed to form colostrum; 3) ) Slowly and evenly add the asiaticoside-chitosan acetic acid solution to the colostrum, stir, and fully emulsify; 4) Slowly and evenly add the glyceraldehyde solution to cross-link and solidify, centrifuge, and the precipitate is washed with petroleum ether successively. washing, washing with absolute ethanol, and vacuum freeze-drying to obtain light yellow powder of asiaticoside-sodium alginate-chitosan microspheres.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an asiaticoside-chitosan-sodium alginate microsphere, a preparation method and an application thereof. Background technique [0002] Asiaticoside (ASiaticoside, AS) is a triterpenoid saponin compound, the main active ingredient of the traditional Chinese medicine Centella asiatica, which has excellent effects of promoting wound healing and anti-hypertrophic scar; Experiments on arthritis found that asiaticoside can inhibit the generation of arthritis in mice, the specific mechanism of action may be by inhibiting the expression of COX-2, reducing the expression and release of inflammatory factors such as IL-6 and TNF-α, so as to inhibit the Proliferation of lymphocytes, thereby inhibiting the occurrence of immune responses. In the experiment of lipopolysaccharide-induced fever in rats, researchers found that asiaticoside can inhibit the expression of COX-2, inhibit t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K9/50A61K9/19A61K47/36A61K31/7024A61P17/02
CPCA61K9/5036A61K9/19A61K47/36A61K31/7024A61P17/02A61K9/5089
Inventor 孔松芝权维燕蔡婷婷李思东郭家棋叶伟霞
Owner GUANGDONG OCEAN UNIVERSITY
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