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Nano-drug for preventing or treating cerebral ischemia-reperfusion injury as well as preparation method and application of nano-drug

A technology of cerebral ischemia reperfusion and preparation process, which is applied in the field of drug preparation to achieve the effects of inhibiting inflammation, inhibiting brain inflammation, and inhibiting excessive activation

Active Publication Date: 2021-02-19
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the 2018 ASA / AHA guidelines do not recommend the use of neuroprotective agents because, so far, there is insufficient evidence that they improve clinical outcomes after ischemic stroke

Method used

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  • Nano-drug for preventing or treating cerebral ischemia-reperfusion injury as well as preparation method and application of nano-drug
  • Nano-drug for preventing or treating cerebral ischemia-reperfusion injury as well as preparation method and application of nano-drug
  • Nano-drug for preventing or treating cerebral ischemia-reperfusion injury as well as preparation method and application of nano-drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Preparation materials and methods of polydopamine nanoparticles

[0035] 1. Main reagents and consumables

[0036]

[0037] 2. Main experimental equipment

[0038]

[0039] 3. Preparation of polydopamine nanoparticles

[0040] (1) Configure weak alkaline reaction buffer solution

[0041] In an open round-bottomed flask, first mix 8 mL of absolute ethanol and 18 mL of deionized water evenly, then add 150 μL of ammonia water, and stir with a magnetic stirrer at room temperature for 30 min to prepare the ammonia water-ethanol mixed solution. The final volume of the mixed solution is About 25mL.

[0042] (2) Dopamine self-polymerization reaction

[0043] Weigh 100 mg of dopamine hydrochloride powder and add 25 mL of ammonia water-ethanol mixed solution reaction buffer prepared in advance, and keep stirring at room temperature for 30 hours. It can be found that the color of the solution gradually changes from colorless to brown, and then gradually tur...

Embodiment 2

[0049] Example 2: Effects of Polydopamine Nanoparticles on Liver and Kidney Tissue Functions

[0050] 1. Experimental animals

[0051] 24 male SD rats. The rats were placed in a standard feeding environment, the ambient temperature was controlled at (22-24° C.), the humidity was 60%, the light time and the dark time were controlled alternately every 12 hours, and the rats had free access to standard food and clean drinking water.

[0052] 2. Experimental method:

[0053] The experimental animals were divided into cages at random, and the administration was started after 3 days of adaptive feeding. For the method, see the group and the administration plan.

[0054] 3. Group and dosing regimen:

[0055] 24 rats were randomly divided into 4 groups according to different doses of PDA: low dose group (5mg / kg), middle dose group (10mg / kg), high dose group (20mg / kg), blank control group (equal volume physiological brine). After only one intraperitoneal drug injection, the rats w...

Embodiment 3

[0058] Example 3: Observation of the cytotoxic effect of polydopamine nanoparticles on rats

[0059] 1. Experimental cells:

[0060] Rat adrenal pheochromocytoma cell PC12, rat renal endometrial cell HBZY-1, rat normal liver cell BRL-3A, and the above cell lines were provided by Jiangsu KGI Biotechnology Co., Ltd.

[0061] 2. Experimental method:

[0062] Digest and count the cells, prepare cell suspension (PC12 5.0×104 cells / mL, HBZY-1, BRL-3A 3.5×104 cells / mL), add 100 μL cell suspension to each well of a 96-well cell culture plate;

[0063] Place the 96-well cell culture plate at 37°C, 5% CO 2 Cultivate in the incubator for 24 hours;

[0064] Dilute the drug with the medium to the required concentration of the working solution, add 100 μL of the corresponding drug-containing medium to each well at 0, 24, 48, and 60 hours respectively, and make 3 duplicate wells, and set up a negative control group at the same time;

[0065] Place the 96-well cell culture plate at 37 °C,...

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Abstract

The invention relates to a nano-drug for preventing or treating cerebral ischemia-reperfusion injury as well as a preparation method and application of the nano-drug. Cerebral ischemia is a cerebrovascular disease which has the greatest threat to human beings, and oxidative injury, inflammatory response and subsequent excitotoxic cell death can be caused by reperfusion as soon as possible. According to the nano-drug, dopamine is oxidized under an alkaline condition to form dopamine quinone, the dopamine quinone is further oxidized to form polydopamine nanoparticles with supramolecular structures, the formed particles are smooth and round in surface, good in sphericity degree and uniform in particle size distribution, a good dispersion state is kept in a solution, and through in-vitro and in-vivo experiments, the polydopamine nanoparticles prove the biological safety. Application of the polydopamine nanoparticles to a cerebral ischemia model shows that the polydopamine nanoparticles canrelieve cerebral ischemia-reperfusion injury, inhibit inflammatory reaction, inhibit activation of an NF-kappa B signal channel and inhibit excessive activation of microglial cells, and have the potential of inhibiting cerebral inflammatory reaction and relieving cerebral ischemia-reperfusion injury.

Description

technical field [0001] The invention relates to a nano-medicine for preventing or treating cerebral ischemia-reperfusion injury, a preparation method and application thereof, and belongs to the field of medicine preparation. Background technique [0002] Stroke is a group of partial or comprehensive brain dysfunction syndrome caused by acute cerebral circulation disorder, which causes about 6.2 million deaths every year, and is the second cause of death and the first cause of disability in the world. Stroke is divided into ischemic stroke and hemorrhagic stroke. Ischemic stroke, also known as cerebral infarction, is the main manifestation of stroke, accounting for about 70-80%. The TOAST classification system divides strokes into large artery atherosclerotic stroke, cardioembolic stroke, small artery occlusive stroke (lacunar stroke), stroke of other definite etiology, and stroke of unknown etiology. Hemorrhagic stroke, also known as intracranial hemorrhage, has a lower in...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K31/785A61P9/10
CPCA61K9/51A61K31/785A61P9/10
Inventor 李斌杨建军纪木火刘盼苗
Owner SOUTHEAST UNIV
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