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A pxcg2 strain of carnobacter maltia aromaticum of diamondback moth and its application

The technology of Carnobacterium aromaticum and diamondback moth is applied in the field of agricultural microorganisms to achieve the effects of good biological control potential and application prospects, and improving insecticidal activity.

Active Publication Date: 2022-06-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, patent CN110982729A discloses a composite bacterial agent for degrading wood processing by-product poplar bark cellulose, wherein the active ingredient of the bacterial agent includes Carnobacterium maltaromaticum strain TMW2.158; patent CN101155910 discloses a Carnobacterium maltaromaticum or related fermented products or bacteriocin compositions are used to treat food, for example, they can be used to treat fresh or processed meat to resist bacterial contamination; the above-mentioned patents all involve related functional research on Carnobacterium maltaromaticum bacteria, but There are few reports about its effect on the control of Lepidoptera pests and the insecticidal activity of Bt toxin

Method used

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  • A pxcg2 strain of carnobacter maltia aromaticum of diamondback moth and its application
  • A pxcg2 strain of carnobacter maltia aromaticum of diamondback moth and its application
  • A pxcg2 strain of carnobacter maltia aromaticum of diamondback moth and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and culture of strain PxCG2

[0024] (1) Preparation of selective separation medium:

[0025] LB medium (10g peptone, 5g yeast extract, 10g NaCl, 15g agar, dissolved in 1L sterile ddH2O water, adjust pH to 7.0);

[0026] Nutrient agar medium NA (3 g beef extract, 10 g peptone, 5 g NaCl, 15 g agar, dissolved in 1 L sterile ddH2O, adjusted to pH 7.0).

[0027] (2) Dissection and plating: Dissect the 3rd instar larvae of Plutella xylostella in an ultra-clean bench under sterile conditions and take the contents of the midgut, centrifuge to separate the supernatant and precipitate, and dilute to 5 concentration gradients, according to the dilution of 10-4 After -10-5 times, the NA plates were respectively coated, placed in a 30°C constant temperature incubator, and observed every 24 hours;

[0028] (3) Continuous purification and culture and photographing: After a single colony grows in the selective medium, first pick a single colony according to the c...

Embodiment 2

[0029] Example 2 Identification and phylogenetic analysis of strain PxCG2

[0030] 1. Traditional biological identification

[0031] (1) Morphological characteristics of colonies

[0032] From the morphological point of view, PxCG2 bacteria are round white colonies with smooth and glossy surface, single, paired or chain-like slender bacilli. Heterofermentation, mainly L(+)-lactic acid is produced from glucose. The optimum growth temperature is 30 °C, aerobic growth, and slow growth under anaerobic conditions.

[0033] (2) Determination of Physiological and Biochemical Characteristics of Carnotella maltata

[0034] Bacterial physiology and biochemical assays were carried out with reference to the method in the Manual for the Identification of Common Bacterial Systems, using bacterial microbiochemical reaction tubes. The test results of available carbon sources are shown in Table 1. Galactose, sorbitol, rhamnose, arabinose, xylose, melibiose, D-ribose, sucrose, and yellow pi...

Embodiment 3

[0043] Example 3 Synergistic effect of PxCG2 strain on Bt

[0044] 1. Removal of intestinal bacteria of diamondback moth

[0045] Prepare antibiotic solution: 1mg / mL ciprofloxacin; 1mg / mL levofloxacin; 2mg / mL metronidazole;

[0046] Methods for the elimination of intestinal bacteria of the diamondback moth, and the addition of antibiotics. Specific method: Collect and sterilize the egg card of diamondback moth: collect the egg card produced by the diamondback moth in its peak spawning period, first place it in 5‰ sodium hypochlorite disinfectant for 10 minutes, and then soak it in clean water twice, 5 times each time minutes, and finally dried with absorbent paper and placed in a worm box, and fed with feed after 24 hours;

[0047] Antibiotic feeding treatment: Weigh 4 g of diamondback moth artificial feed into a sterile petri dish, then add more than 333 μL of the prepared ciprofloxacin, levofloxacin and metronidazole antibiotic solutions, mix well and place them in a worm ...

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Abstract

The invention discloses a Carnobacterium maltaromaticum PxCG2 strain of diamondback moth and its application. The strain was preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on August 11, 2020, and the strain preservation number is GDMCC No: 61133. The research of the present invention shows that the Carnobacterium maltaromaticum PxCG2 strain has the effect of improving the insecticidal activity of Bt toxin and synergizing the effect of Cry1Ac protoxin to quickly kill diamondback moth. The Carnobacterium maltaromaticum PxCG2 strain can be used as a new type of biological control bacteria for the prevention and control of cruciferous vegetable pests , has good biological control potential and application prospects.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, and more particularly, to a Carnobacterium maltaromaticum PxCG2 strain of diamondback moth and its application. Background technique [0002] The diamondback moth, Plutella xylostella (L), is one of the most serious pests of cruciferous crops, often causing serious economic losses to many important vegetables such as cabbage, broccoli and cauliflower (Talekar and Shelton, 1993). The diamondback moth has developed severe resistance to all insecticides used to control lepidopteran pests, as well as B. thuringiensis (Bt), which is increasingly difficult to control (Talekar and Shelton, 1993; Baxter et al., 2005 ). Therefore, it is necessary to find a method that can solve or alleviate the resistance of diamondback moth to Bt, thereby improving the insecticidal activity of Bt protein. [0003] There are different types and numbers of insect symbiotic bacteria distributed in vari...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01N63/50A01P1/00A01P7/04C12R1/01
CPCA01N63/20A01N63/50C12R2001/01C12N1/205
Inventor 金丰良许小霞李树忠付东冉
Owner SOUTH CHINA AGRI UNIV