Unlock instant, AI-driven research and patent intelligence for your innovation.

Rhodiola rosea extract as well as preparation method and application thereof

A technology of Rhodiola rosea and its extracts, which can be applied in the fields of pharmaceutical formulations, cosmetic preparations, dressing preparations, etc. It can solve the problems of restricting the application of Rhodiola rosea, different fermentation conditions, and low melanin inhibition ability, and achieve the production process. The effect of simple process, increased tyrosol content, and enhanced ATP production capacity

Pending Publication Date: 2021-03-12
上海瑞帝安生物科技有限公司
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the fermentation of Rhodiola rosea, there are few studies on the fermentation process using Rhodiola rosea as a raw material, and the application of fermentation product extracts in cosmetics, and there are many types of microbial starters (Hong Xidao et al. September 10, 2014 The patent applied for "Manufacturing Method of Rhodiola Rosea Fermentation Using Adsorbent"; Patent Publication No. CN103131730B), the fermentation conditions are different, which greatly limits the application of Rhodiola Rosea in the fields of whitening, anti-aging and anti-oxidation
Beijing Technology and Business University and Shanghai Yidai Cosmetics Co., Ltd. applied for the Chinese patent "A Rhodiola Fermented Protoplasm Cosmetic and Its Preparation Method" on August 24, 2016. The patent announcement number is CN104257545B. Yeast is used to process Rhodiola root Whole plant fermentation retains all the functional components and activities of the plant. The product is rich in salidroside, arbutin, etc., which have the activity of inhibiting tyrosinase activity and melanin synthesis, and the melanin inhibition ability is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhodiola rosea extract as well as preparation method and application thereof
  • Rhodiola rosea extract as well as preparation method and application thereof
  • Rhodiola rosea extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Rhodiola rosea fermentation method and functional assay method

[0041] 1 Materials and methods

[0042] material name buy manufacturers Lactobacillus delbrueckii freeze-dried powder Shanghai Fuxiang Biotechnology Co., Ltd. Rhodiola rosea (origin Sichuan) Hebei Golden Leaf Pharmaceutical Co., Ltd. MRS Broth Medium Hangzhou Best Biotechnology Co., Ltd. SK-MEL-2 (human cutaneous malignant melanoma) cells Shanghai Aolu Biotechnology Co., Ltd. B16F10 (mouse melanoma) cells Shanghai Jingkang Biological Engineering Co., Ltd. BYJC0090 ultra-trace ATPase test kit Shanghai Kemin Biotechnology Co., Ltd. human skin fibroblasts Shanghai Xinyu Biotechnology Co., Ltd.

[0043] 1.1. Grind Rhodiola rosea into powder (20-80 mesh) with a pulverizer, and then use it after autoclaving. Sterilization conditions: 121°C, 30min.

[0044] 1.2, Culture medium preparation: Take 49.25g of MRS broth, add 1L of distilled w...

Embodiment 2

[0060] The functional measurement of embodiment 2 comparative example

[0061] 2.1: Determination of salidroside and tyrosol content

[0062] For the unfermented Rhodiola rosea extract (comparative example), different gradient solutions were prepared after adding methanol solution (chromatographic grade), and used after filtering with a 0.2 μm filter membrane. Add the pure products of salidroside and tyrosol to methanol solution (chromatographic grade) to configure different gradient solutions, establish a standard curve, and refer to the steps of 1.6 in Example 1 to determine salidroside and tyrosol in the unfermented Rhodiola rosea extract. Alcohol content.

[0063] 2.2: Melanin Determination

[0064] According to the steps of 1.7 in Example 1, the melanin of human malignant melanoma cells and the melanin of mouse melanoma cells were measured, and the melanin and melanin of human malignant melanoma cells were measured with different concentrations of unfermented Rhodiola r...

Embodiment 3

[0067] Fermentation and extraction of Rhodiola rosea under various different conditions of embodiment 3

[0068] Carry out the preparation of Rhodiola rosea fermentation product extract according to the step of embodiment 1, after Rhodiola rosea is pulverized, join in the sterilized liquid medium, and inoculate and cultivate Lactobacillus delbrueckii, the ratio is Rhodiola rosea powder: Liquid culture medium: bacterial liquid=1:2-3:0.5. The culture temperature was set at 37±3°C, 70% ethanol was used as the extraction solvent, and the extraction time was 3 hours. For specific steps, see figure 2 , concentrated and centrifuged to obtain the supernatant, and the filtrate obtained by filtering with 5C filter paper is the Rhodiola rosea fermentation product extract. Fermentation culture time and conditions are different, as shown in the table below:

[0069] Extract of the present invention Fermentation time Rhodiola rosea powder: culture medium: bacterial liquid ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a rhodiola rosea extract as well as a preparation method and an application thereof. The rhodiola rosea fermentation extract is a rhodiola rosea fermentation primary extract obtained by sterilizing fermentation liquor obtained by mixing and fermenting lactobacillus delbrueckii, rhodiola rosea root powder and a culture medium and extracting with solutions such as ethanol andthe like; and centrifugally filtering to obtain the rhodiola rosea extract; wherein the volume concentration of the extracting solution is 30-80%. The rhodiola rosea fermentation extract obtained by the preparation process disclosed by the invention is remarkably improved in tyrosol content, whitening and antioxidant effects and ATP productivity, is convenient to prepare and relatively low in cost, and can be directly added into cosmetics.

Description

technical field [0001] The invention relates to a cosmetic raw material, in particular to a Chinese herbal medicine extract and a preparation method. Background technique [0002] In recent years, Rhodiola rosea extract has anti-tumor, anti-aging, antibacterial, anti-oxidation, anti-fatigue effects, and can effectively enhance the immune function of the body (Xie Zhaohui, August 7, 2018, applied for a Chinese patent "a kind of Moisturizing anti-radiation whitening skin care product and its preparation method”, Patent Publication No. CN 108635308A), in which salidroside and salidroside aglycone tyrosol can improve the body’s anti-aging ability by improving free radical metabolism and increase the SOD activity in cells reduces the content of lipid peroxide LPO and delays cell aging, making Rhodiola extract more and more used in the fields of food, medicine and cosmetics. Rhodiola (scientific name: Rhodiola rosea L.), It is a perennial herb that is distributed all over my country...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/9789A61Q19/00A61Q19/02A61Q19/08A61K2800/85
Inventor 魏茂林李亨载陈超金恩智
Owner 上海瑞帝安生物科技有限公司