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Application of bispyridine thioketone in prevention and treatment of microbial diseases

A bispyridinethione and microorganism technology, which is applied in the directions of chemicals, applications, biocides for biological control, etc., can solve the problems of endangering food safety, bad changes in food odor and tissue structure, serious drug resistance of bacteria, etc. To achieve the effect of excellent antibacterial activity

Inactive Publication Date: 2021-03-16
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, some microorganisms are corrupt and can cause adverse changes in food odor and tissue structure, which is the main factor that endangers food safety.
Chemical fungicides and antibiotics are currently the most important strategies for the prevention and treatment of pathogenic microorganisms. However, due to the extensive abuse of these agents, the resistance of bacteria is becoming more and more serious, and the control effect of existing agents is declining.

Method used

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  • Application of bispyridine thioketone in prevention and treatment of microbial diseases
  • Application of bispyridine thioketone in prevention and treatment of microbial diseases
  • Application of bispyridine thioketone in prevention and treatment of microbial diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1: Determination of indoor antifungal activity

[0010] Mycelia growth rate method: According to a certain concentration gradient, add the test compound into the medium that has been sterilized and cooled to about 50°C, mix well, and dilute the agent into a series of concentrations to prepare a drug-containing medium. Each petri dish Inoculate a bacterial dish (diameter d=5mm), and set 3 replicates for each concentration. Cultivate at 25°C until the control colony covers more than 2 / 3 of the petri dish, measure the colony diameter (mm) of each concentration treatment by the cross method, calculate the growth inhibition rate of the drug on the mycelium, and use statistical software SAS8 for statistical analysis of differences .1 (Statistical Analysis System 8.1). The inhibitory effect of dipyridinethione on 6 kinds of phytopathogenic fungi and 2 kinds of molds was determined, and the results are shown in Table 1.

[0011] Table 1. Inhibitory activity of bisp...

Embodiment 2

[0014] Embodiment 2: Indoor antibacterial activity assay

[0015] Micro-broth dilution method: Inoculate agricultural bacteria or human pathogenic bacteria in 20mL MB medium, and then incubate in a constant temperature shaker at 37°C for 12h with a rotation speed of 150rpm. Take an appropriate amount of bacterial culture solution and use a microplate reader to measure the absorbance at 600nm, and use the culture solution without bacteria as a blank control. 600 0.1 is equivalent to a concentration of 1×10 8 cfu / mL, the bacterial suspension was diluted to a concentration of 1×10 with MH medium 6 cfu / mL bacterial solution, set aside.

[0016] Use a row gun to draw 50 μL of the diluted bacterial solution into a 96-well plate, and then add 50 μL of the compound solution in sequence. The blank control is the addition of the same volume of PBS solution, and the positive control is eclotriazole. Three replicates are set for each concentration. After the samples were incubated in...

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Abstract

The invention discloses an application of bispyridine thioketone in prevention and treatment of microbial diseases. The bispyridine thioketone can be used for preventing and treating diseases caused by sclerotinia sclerotiorum, rhizoctonia solani, botrytis cinerea, fusarium graminearum, cotton fusarium oxysporum, magnaporthe grisea, aspergillus flavus, aspergillus niger, citrus canker, potato black shank, escherichia coli and candida albicans. Bispyrithione shows excellent antibacterial activity on most pathogenic bacteria, and can be used for development of bactericidal lead molecules.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, and discloses a new application of dipyridinethione, in particular to its use in the prevention and treatment of Sclerotinia sclerotiorum, Rhizoctonia solani, Botrytis cinerea, wheat scab, cotton fusarium wilt, and rice blast fungus, Aspergillus flavus, Aspergillus niger, canker citrus, potato blackleg, Escherichia coli, and Candida albicans. Background technique [0002] Pathogenic microorganisms mainly include viruses, bacteria, fungi, protozoa and worms. Pathogenic microorganisms are widely distributed and can infect humans, animals and plants, induce diseases and allergies, lead to crop death, food production reduction, food spoilage, etc., seriously endangering human health, public health safety, food and food safety. For example, colibacillosis is an infectious disease caused by the pathogen Escherichia coli, mainly relying on bacteria and its metabolites to cause disease, such as diarrh...

Claims

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Application Information

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IPC IPC(8): A01N43/40A01P1/00A01P3/00
CPCA01N43/40
Inventor 刘映前张智军贺颖慧吴天琳杜莎莎赵文斌
Owner LANZHOU UNIVERSITY
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