Application of alcaligenes faecalis in preparation of microbial agent for efficiently degrading ammonia nitrogen and nitrite nitrogen in aquaculture water
A technology of Alcaligenes faecalis and microbial inoculum, which is applied in the field of microorganisms, can solve the problems of loss of aquaculture farmers, weakening of the ability of aquaculture organisms to transport oxygen in blood, and mass death of aquaculture organisms, so as to increase the effect and income of aquaculture and reduce aquatic products. Breeding risks and the effect of ensuring the ecological health of water bodies
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Embodiment 1
[0041] Example 1: Screening, isolation and identification of Alcaligenes faecalis GBW-HB1905
[0042] 1. Screening and purification of Alcaligenes faecalis GBW-HB1905
[0043] Take 15ml of mixed sewage to be treated from a municipal sewage plant, add it to 135mL of PBS buffer solution, oscillate for 20 minutes to fully mix the sample, centrifuge at 1000rpm for 1min, collect the sample supernatant, and set aside. Take 1 mL of the supernatant of the above sample, add it to an Erlenmeyer flask filled with 100 mL of high-salinity ammonium nitrogen degrading bacteria enrichment and screening liquid medium, culture at 28 °C, 180 rpm constant temperature shaker for 2 days, and enrich for 3 times. After gradient dilution of the cultured bacterial suspension, 100 μL was spread on the high-salinity ammonium nitrogen degrading bacteria enrichment and screening solid medium, and placed in a 28°C incubator for cultivation. After 48 hours, single colonies of different shapes were picked an...
Embodiment 2
[0048] Example 2: Growth determination and physiological and biochemical characteristics of Alcaligenes faecalis GBW-HB1905
[0049] 1. Growth determination of Alcaligenes faecalis GBW-HB1905
[0050] Inoculate the Alcaligenes faecalis GBW-HB1905 cultured on the slant into the improved NB medium, and culture it in a constant temperature shaker with a pH of 7.0-7.5 and a temperature of 28°C for 22 hours to prepare a GBW-HB1905 bacterial liquid, wherein every 1.0 hours Take a sample once, measure the absorbance value at OD600nm, and draw the growth curve. Such as figure 2 As shown, the experimental results show that GBW-HB1905 is in the growth retardation phase in the first 4 hours of culture, and then enters the logarithmic growth phase, and enters the logarithmic growth phase after 14-16 hours of culture, and the number of bacteria reaches 6.0×10 9 CFU / mL, 16-20h is the stable growth period, and then enters the decline period, thus completing the entire growth cycle.
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Embodiment 3
[0056] Example 3: Salinity tolerance test of Alcaligenes faecalis GBW-HB1905
[0057] In order to test the survival performance of Alcaligenes faecalis GBW-HB1905 under high salinity conditions, its growth under different salinity conditions was analyzed and detected. Adjust the amount of sodium chloride in the NB medium to prepare medium with different salt concentrations, and then inoculate Alcaligenes faecalis GBW-HB1905 cultured on the slant into NB medium with different salt concentrations, at pH7.0-7.5, The culture solution of Alcaligenes faecalis GBW-HB1905 was obtained by culturing in a constant temperature shaker at 28°C for 18 hours, and the growth status of Alcaligenes faecalis GBW-HB1905 in media with different salt concentrations was detected. The results are shown in Table 2. GBW-HB1905 has good salt-tolerant growth characteristics, and can grow well in the salinity range of 15-35‰.
[0058] Table 2 Growth of Alcaligenes faecalis GBW-HB1905 in different salt con...
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