Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of alcaligenes faecalis in preparation of microbial agent for efficiently degrading ammonia nitrogen and nitrite nitrogen in aquaculture water

A technology of Alcaligenes faecalis and microbial inoculum, which is applied in the field of microorganisms, can solve the problems of loss of aquaculture farmers, weakening of the ability of aquaculture organisms to transport oxygen in blood, and mass death of aquaculture organisms, so as to increase the effect and income of aquaculture and reduce aquatic products. Breeding risks and the effect of ensuring the ecological health of water bodies

Active Publication Date: 2021-03-16
QINGDAO SHANGDE BIOTECH
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, the harm of ammonia nitrogen to aquatic organisms mainly comes from the harm of non-ionic ammonia. After non-ionic ammonia enters the aquatic organisms, it will have a significant impact on their metabolic reactions and membrane stability, resulting in dyspnea and non-feeding of the cultured organisms. , decreased resistance, convulsions, and coma, etc., will affect the biochemical indicators and health status of aquatic organisms, and in severe cases will also lead to the mass death of aquaculture organisms; and nitrite poisoning will directly affect and weaken the ability of the blood of aquaculture organisms to transport oxygen , leading to symptoms of hypoxia in the cultured organisms, resulting in abnormal appearance and internal organs, and then death
Therefore, the excessive presence of these two substances will seriously affect the health of aquatic organisms in the aquaculture water, and they are two "invisible killers" in aquaculture. If the content is too high, it is likely to cause heavy losses to aquaculture farmers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of alcaligenes faecalis in preparation of microbial agent for efficiently degrading ammonia nitrogen and nitrite nitrogen in aquaculture water
  • Application of alcaligenes faecalis in preparation of microbial agent for efficiently degrading ammonia nitrogen and nitrite nitrogen in aquaculture water
  • Application of alcaligenes faecalis in preparation of microbial agent for efficiently degrading ammonia nitrogen and nitrite nitrogen in aquaculture water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Screening, isolation and identification of Alcaligenes faecalis GBW-HB1905

[0042] 1. Screening and purification of Alcaligenes faecalis GBW-HB1905

[0043] Take 15ml of mixed sewage to be treated from a municipal sewage plant, add it to 135mL of PBS buffer solution, oscillate for 20 minutes to fully mix the sample, centrifuge at 1000rpm for 1min, collect the sample supernatant, and set aside. Take 1 mL of the supernatant of the above sample, add it to an Erlenmeyer flask filled with 100 mL of high-salinity ammonium nitrogen degrading bacteria enrichment and screening liquid medium, culture at 28 °C, 180 rpm constant temperature shaker for 2 days, and enrich for 3 times. After gradient dilution of the cultured bacterial suspension, 100 μL was spread on the high-salinity ammonium nitrogen degrading bacteria enrichment and screening solid medium, and placed in a 28°C incubator for cultivation. After 48 hours, single colonies of different shapes were picked an...

Embodiment 2

[0048] Example 2: Growth determination and physiological and biochemical characteristics of Alcaligenes faecalis GBW-HB1905

[0049] 1. Growth determination of Alcaligenes faecalis GBW-HB1905

[0050] Inoculate the Alcaligenes faecalis GBW-HB1905 cultured on the slant into the improved NB medium, and culture it in a constant temperature shaker with a pH of 7.0-7.5 and a temperature of 28°C for 22 hours to prepare a GBW-HB1905 bacterial liquid, wherein every 1.0 hours Take a sample once, measure the absorbance value at OD600nm, and draw the growth curve. Such as figure 2 As shown, the experimental results show that GBW-HB1905 is in the growth retardation phase in the first 4 hours of culture, and then enters the logarithmic growth phase, and enters the logarithmic growth phase after 14-16 hours of culture, and the number of bacteria reaches 6.0×10 9 CFU / mL, 16-20h is the stable growth period, and then enters the decline period, thus completing the entire growth cycle.

[00...

Embodiment 3

[0056] Example 3: Salinity tolerance test of Alcaligenes faecalis GBW-HB1905

[0057] In order to test the survival performance of Alcaligenes faecalis GBW-HB1905 under high salinity conditions, its growth under different salinity conditions was analyzed and detected. Adjust the amount of sodium chloride in the NB medium to prepare medium with different salt concentrations, and then inoculate Alcaligenes faecalis GBW-HB1905 cultured on the slant into NB medium with different salt concentrations, at pH7.0-7.5, The culture solution of Alcaligenes faecalis GBW-HB1905 was obtained by culturing in a constant temperature shaker at 28°C for 18 hours, and the growth status of Alcaligenes faecalis GBW-HB1905 in media with different salt concentrations was detected. The results are shown in Table 2. GBW-HB1905 has good salt-tolerant growth characteristics, and can grow well in the salinity range of 15-35‰.

[0058] Table 2 Growth of Alcaligenes faecalis GBW-HB1905 in different salt con...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides application of alcaligenes faecalis in preparation of a microbial agent for efficiently degrading ammonia nitrogen and nitrite nitrogen in aquaculture water. The microbial agentcomprises alcaligenes faecalis powder with the bacterium content not lower than 5.0 * 10<8>CFU / g and a carrier, wherein the mass ratio of the alcaligenes faecalis powder to the carrier is 1: (50-100). The alcaligenes faecalis powder is prepared from alcaligenes faecalis GBW-HB1905 with the preservation number of CGMCC No. 20364. The bacterium has wide salt resistance, and the growth salinity range is 15-35 thousandths. The microbial agent has the capacity of efficiently and rapidly degrading ammonia nitrogen and nitrite nitrogen in aquaculture water, can continuously and stably maintain ammonia nitrogen and nitrite nitrogen within a low concentration range, can effectively solve and improve the problem of high concentration of ammonia nitrogen and nitrite nitrogen in aquaculture water, and is beneficial to aquaculture and income.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to the application of Alcaligenes faecalis in the preparation of microbial bacterial agents for efficiently degrading ammonia nitrogen and nitrite nitrogen in aquaculture water. Background technique [0002] In recent years, with the continuous improvement of aquaculture level, the aquaculture density is also increasing while pursuing higher economic benefits. Therefore, how to ensure the health of aquaculture water quality under high-load aquaculture density is particularly critical, because the aquaculture density varies. Enlargement will inevitably increase the amount of bait fed to the pond body, and the resulting residual bait and undigested organic matter content will also continue to increase, exceeding the self-repair capacity of the water body, especially in shrimp farming, the protein content of the bait is more abundant , more organic nitrogen sources will remain in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N11/14C02F3/34C02F101/16C02F103/20C12R1/05
CPCC12N1/20C12N11/14C02F3/34C02F2101/16C02F2101/166C02F2103/20
Inventor 袁绍辉王熙涛王金龙赵金超张晓春
Owner QINGDAO SHANGDE BIOTECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More