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Photo-crosslinking/electrostatic spinning preparation and application of hydrogel composite scaffold with double-layer skin structure

A composite scaffold and skin structure technology, applied in textiles and papermaking, filament/thread molding, single-component protein rayon, etc., can solve the problem of single function, achieve good air permeability, promote cell migration and proliferation, and promote The effect of regeneration and formation

Active Publication Date: 2021-03-30
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to aim at the wound dressing in the prior art, which has a single function, and has the advantages of biocompatibility, degradability, cell adhesion, air permeability, degree of fit with the wound, nutrient transport, and wound healing. Defects and deficiencies, provide a double-layer skin structure hydrogel composite scaffold prepared by photocrosslinking / electrospinning

Method used

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  • Photo-crosslinking/electrostatic spinning preparation and application of hydrogel composite scaffold with double-layer skin structure
  • Photo-crosslinking/electrostatic spinning preparation and application of hydrogel composite scaffold with double-layer skin structure
  • Photo-crosslinking/electrostatic spinning preparation and application of hydrogel composite scaffold with double-layer skin structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Preparation of double-layer skin structure hydrogel composite scaffold by photocrosslinking / electrospinning

[0063] 1. Preparation of gelatin electrospun fiber membrane

[0064] Preparation of electrospinning solution: Weigh 0.5 g of gelatin with a microelectronic balance and dissolve in 5 mL of hexafluoroisopropanol (HFIP) in a glass bottle (10 mL), add a magnetic stirrer, place on a magnetic stirrer, and magnetically stir for 24 hours. use.

[0065] Electrospinning method: the electrospinning experiment is carried out at room temperature and humidity below 50%. The positive electrode high-pressure injection head adopts 8# flat needle (length 20cm), the voltage regulation is 16kV, the distance from the needle to the receiving device is 20cm, the propulsion speed is 16.7μL / min, and the volume of the electrospinning solution is 320μL. Spread aluminum foil paper on a self-assembled platform with a size of 10cm×10cm, and place several 15mm glass slides for sam...

Embodiment 2

[0088] Example 2 Performance and function test of double-layer skin structure hydrogel composite scaffold

[0089] 1. The appearance of the prepared double-layer skin structure hydrogel composite scaffold is as follows: figure 1 shown.

[0090] From figure 1 It can be seen that the prepared double-layer skin structure hydrogel composite scaffold is hydrogel-like, in which the gelatin electrospun fiber membrane is the base layer, and the cylindrical gelatin hydrogel is uniformly arranged on it, and the cylindrical hydrogel keep a certain interval between them.

[0091] The overall thickness of the composite scaffold prepared in Example 1 is 350 μm; the diameter of the cylindrical hydrogel is 600 μm; the height is 300 μm.

[0092] The diameter of the hydrogel can be further adjusted by controlling the size of the pores in the mask. However, in order to ensure the air permeability of the composite scaffold and the fit of the skin, the diameter of the cylindrical hydrogel is c...

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Abstract

The invention discloses photo-crosslinking / electrostatic spinning preparation and an application of a hydrogel composite scaffold with a double-layer skin structure. According to the composite scaffold, a gelatin electrostatic spinning fiber membrane is used as a substrate, and cylindrical gelatin hydrogel is prepared on one surface of the gelatin electrostatic spinning fiber membrane through a photo-crosslinking effect, so that the hydrogel composite scaffold with the double-layer skin structure is obtained. The composite scaffold has a double-layer structure, the fiber membrane is equivalentto the epidermal layer of the skin, foreign matter and pathogen invasion can be blocked, and tissue fluid loss can be prevented; the patterned cylindrical gelatin not only can support cell viabilityand cell adhesion, but also can promote cell migration and proliferation and promote regeneration and formation of skin tissues; due to the unique shape, the composite scaffold has good air permeability, large porosity and pore diameter while good fitness with wounds is guaranteed, transportation of oxygen and nutrient substances of the wounds is facilitated, generation of blood vessels at the wounds can be promoted, the degradation speed of the composite scaffold is close to the wound healing period, and wound healing is facilitated.

Description

technical field [0001] The invention relates to the technical field of biological tissue scaffolds, more specifically, to the photocrosslinking / electrospinning preparation and application of a double-layer skin structure hydrogel composite scaffold. Background technique [0002] The skin is the most vulnerable organ in the human body to external stimuli. It provides a natural barrier between the surrounding environment and the body's internal organs. From a tissue engineering point of view, the most important feature of the skin is its ability to remodel and regenerate itself and replace continuously lost tissue. Anatomically, skin tissue can be divided into three layers: epidermis, dermis, and subcutaneous tissue. For many years, tissue engineers have attempted to construct appropriate full-thickness skin equivalents under in vitro conditions, however, many limitations still exist. [0003] An ideal skin scaffold should be a biodegradable three-dimensional scaffold with ...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/62A61L15/42D01F4/00D06M13/224D04H1/728D01D5/00
CPCA61L15/32A61L15/425A61L15/62A61L15/42D01F4/00D06M13/2246D04H1/728D01D5/0015C08L89/06
Inventor 李燕臧宏运宋婷
Owner SUN YAT SEN UNIV
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