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Microbial organic compound fertilizer for low-temperature environment and preparation method thereof

A low-temperature environment, microbial technology, applied in organic fertilizers, urea compound fertilizers, low-temperature treatment fertilizers, etc., can solve the problems of slow growth and reproduction of microorganisms, difficult to achieve sterilization, stimulate growth, etc., to improve growth speed and health status, improve Growth rate, effect of increasing fertilizer temperature

Pending Publication Date: 2021-04-09
湖北庄康生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a microbial organic compound fertilizer for low-temperature environments and a preparation method thereof, which solves the problem in the prior art that fertilizers are often used in cold regions or when the temperature is low in winter The growth and reproduction speed of microorganisms in the medium is very slow, which makes it difficult to achieve the effects of sterilization and growth stimulation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The microbial organic compound fertilizer in the present embodiment is prepared according to the following steps:

[0041] (1) Preparation of compound fertilizer components: take 15% urea, 30% monoammonium phosphate, and 38% potassium sulfate by weight percentage respectively, break up the above four respectively, and then mix and stir evenly.

[0042] (2) Preparation of microbial components: pick 1-2 rings of bacteria from Bacillus subtilis stored on a slant or frozen in glycerol, inoculate into seed medium, and culture in shake flask at 28°C, 150-250r / min for 10h, Obtain the primary seed solution; inoculate the primary seed solution with an inoculum size of 2%-5% in the liquid expanded LB medium, and cultivate it for 10 hours at 28°C and 180r / min shake flask to obtain the secondary seed solution; in the fermentation medium Inoculate secondary seeds with 2%-5% inoculum amount. Fermentation in a fermenter at a temperature of 28° C. and a stirring paddle rotation speed ...

Embodiment 2

[0048] The microbial organic compound fertilizer in the present embodiment is prepared according to the following steps:

[0049] (1) Preparation of compound fertilizer components: take 28% of urea, 31% of calcium magnesium phosphate fertilizer and 16% of potassium sulfate by weight percentage respectively, break up the above three respectively, and then mix and stir evenly.

[0050] (2) Preparation of microbial components: pick 1-2 rings of bacteria from Brevibacillus lateralsporosa stored on a slant or frozen in glycerol, inoculate into seed medium, and culture in shake flask at 28°C, 150-250r / min 10h, obtain the primary seed liquid; inoculate the primary seed liquid with the inoculation amount of 2%-5% in the liquid expansion LB medium, in 28 ℃, 180r / min shake flask culture 10h, obtain the secondary seed liquid; Inoculate secondary seeds with 2%-5% inoculum in the medium. Ferment in a fermenter at a temperature of 28°C and a stirring paddle speed of 180r / min for 24 hours, ...

Embodiment 3

[0056] The microbial organic compound fertilizer in the present embodiment is prepared according to the following steps:

[0057] (1) Preparation of compound fertilizer components: Weigh 33% of urea, 23% of calcium magnesium phosphate fertilizer and 16% of potassium sulfate by weight percentage respectively, break up the above three respectively, and then mix and stir evenly.

[0058] (2) Preparation of microbial components: pick 1-2 rings of bacteria from the jelly-like Paenibacillus preserved on a slant or frozen in glycerin, inoculate into the seed medium, shake the flask at 28°C, 150-250r / min Cultivate for 10 hours to obtain the primary seed liquid; inoculate the primary seed liquid with an inoculum size of 2%-5% in the liquid expanded LB medium, and cultivate the primary seed liquid at 28°C and 180r / min shake flask for 10 hours to obtain the secondary seed liquid; Secondary seeds are inoculated with 2%-5% inoculum in the fermentation medium. Ferment in a fermenter at a t...

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Abstract

The invention provides a microbial organic compound fertilizer for a low-temperature environment, which comprises a compound fertilizer component, an organic matter component and a microbial component, the compound fertilizer component comprises nitrogen, phosphorus, potassium and trace element components; wherein the microbial component comprises sterilization microorganisms and heating microorganisms. The invention further provides a preparation method of the microbial organic compound fertilizer for the low-temperature environment. The preparation method comprises the steps of compound fertilizer component preparation, microbial component preparation, mixing and granulation. According to the invention, through cooperative use of the sterilization microorganisms and the heating microorganisms, normal decomposition of organic matters and proper propagation of the sterilization microorganisms in a low-temperature environment can be ensured at the same time, so that nutrient substances required by plant growth are increased, and inhibition of harmful bacteria is also ensured; therefore, under the dual effects, the growth speed and the health condition of the plants in a low-temperature environment are greatly improved.

Description

technical field [0001] The invention relates to the technical field of microbial organic compound fertilizers, in particular to a microbial organic compound fertilizer used in a low-temperature environment and a preparation method thereof. Background technique [0002] Chemical fertilizers have laid a material foundation for the production and development of modern agriculture and the protection of human food security. However, the low utilization rate of chemical fertilizers and the resulting high environmental risks have always been a major problem plaguing the world. At present, due to the increase in the use of agriculture and chemical fertilizers around the world, the dependence on pesticides and chemical fertilizers is increasing, resulting in a series of problems such as insufficient use of organic fertilizers, imbalance of soil nutrient ratios, soil compaction, decline in soil quality, and pollution of rivers and groundwater. To avoid repeating the mistakes of develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/60C05G5/12
CPCC05B7/00C05B13/02C05G3/00C05G3/60C05G5/12C05C9/00C05D1/02C05F5/002C05F11/08C05F11/00
Inventor 毛爱华
Owner 湖北庄康生物科技股份有限公司