Technology for continuously producing denitrifying bacteria agent

A nitrifying bacteria agent and denitrification technology, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problem that the best logarithmic growth period of bacteria cannot be guaranteed, and achieve easy promotion and application, improve cultivation efficiency, Ease of large-scale effects

Pending Publication Date: 2021-04-20
华沃德源环境技术(济南)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned cultivation device carries out periodical microbial cultivation through "cultivation-discharging-solid-liquid separation-feeding-cultivation", which is continuous cultivation for sequence batching. During the cultivation process, the concentration of nutrients and the content of bacteria in the cultivation system are in periodic changes. , there is no guarantee that the bacteria are always in the optimal logarithmic growth phase

Method used

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  • Technology for continuously producing denitrifying bacteria agent
  • Technology for continuously producing denitrifying bacteria agent

Examples

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Comparison scheme
Effect test

Embodiment 1

[0029] A process for continuously producing denitrifying bacteria agent, comprising the following steps:

[0030] S1 preparation medium:

[0031] Select carbon source: choose sodium acetate as the carbon source, and its dosage is 2.0g / L;

[0032] Select the nitrate nitrogen source: select sodium nitrate as the nitrogen source, and the dosage is 6.0g / L;

[0033] Choose an organic nitrogen source: choose yeast extract as the nitrogen source, and the dosage is 0.5g / L;

[0034] Select inorganic nutrient salt, choose manganese chloride as inorganic nutrient salt, and its consumption is 0.1g / L;

[0035] S2: Inoculate the denitrifying bacteria, the inoculation amount is 800mg / L, enter the culture medium from the water distributor at the bottom, and after the culture medium enters the reaction column 3 from the original water tank 1, denitrify the bacterial agent; the clip of the reaction column 3 The sleeve realizes the continuous circulation of hot water to heat the interior of t...

Embodiment 2

[0055] A kind of technique for continuously producing denitrifying bacteria agent, comprising the steps of:

[0056] S1 preparation medium:

[0057] Select carbon source: select citric acid as the carbon source, and its dosage is 3.0g / L;

[0058] Select the nitrate nitrogen source: select potassium nitrate as the nitrogen source, and the dosage is 5.0g / L;

[0059] Select organic nitrogen source: select peptone as nitrogen source, the dosage is 0.4g / L;

[0060] Select inorganic nutrient salt, select magnesium sulfate as inorganic nutrient salt, and its consumption is 0.15g / L;

[0061] In S2, the inoculum amount was 600 mg / L, and the temperature was controlled at about 32° C. during the reaction, and the rest were the same as in Example 1.

Embodiment 3

[0063] A kind of technique for continuously producing denitrifying bacteria agent, comprising the steps of:

[0064] S1 preparation medium:

[0065] Select carbon source: select maltose as the carbon source, and its dosage is 3.0g / L;

[0066] Select the nitrate nitrogen source: choose calcium nitrate as the nitrogen source, and the dosage is 4.0g / L;

[0067] Select the organic nitrogen source: choose soybean meal as the nitrogen source, and the dosage is 0.4g / L;

[0068] Select inorganic nutrient salt, select copper sulfate as inorganic nutrient salt, and its consumption is 0.15g / L;

[0069] In S2, the inoculum amount was 600 mg / L, and the temperature was controlled at about 32° C. during the reaction, and the rest were the same as in Example 1.

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Abstract

The invention belongs to the technical field of microbial agent production and particularly relates to a technology for continuously producing a denitrifying bacteria agent. The technology comprises the following steps: S1, preparing a culture medium; and S2, after an inoculation by denitrifying bacteria, the culture medium in step S1 enters a reaction column from a raw water tank for nitrification culture; controlling a reaction temperature to be 30-35 DEG C and performing continuous refluxing in the process; enabling generated clear water to enter a clear water tank, enabling the denitrifying bacteria generated by proliferation in the culture process to enter a precipitation tank through a sampling valve, and controlling the content of the denitrifying bacteria in the reaction column to be 3,000-5,000 mg/L; and exhausting generated gas through an exhaust pipeline. The technology realizes continuous feeding and discharging of the culture medium, always keeps component content of the culture medium and concentration of the bacteria in a system in a constant state, ensures that the bacteria in the system is in a logarithmic growth phase with a fastest division and proliferation rate, greatly improves culture efficiency and reduces operation difficulty.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agent production, and in particular relates to a process for continuously producing denitrifying bacterial agents. Background technique [0002] CN111875046A A culture medium for directional enrichment of nitrite nitrogen accumulating denitrifying bacteria from activated sludge, characterized in that the culture medium includes: 0.1-1.0g·L -1 Ammonium sulfate, 0.01~0.1g·L -1 Sodium dihydrogen phosphate, 0.1~1.3g·L -1 Calcium chloride, 0.1~0.6g·L -1 Magnesium sulfate heptahydrate, 0.3~2ml·L -1 Trace elements, sodium bicarbonate, sodium nitrate, and sodium acetate; wherein, adding the sodium nitrate and the sodium acetate, so that the COD / NO3 of the medium - -N is in the range of 1.5-2.5; the sodium bicarbonate is added so that the initial pH of the medium is in the range of 8.5-9.0. [0003] The culture method described in the above-mentioned literature is a directional enrichment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 薛飞曹洪亮宋迪刘昱余刚张帅威郭芳坤
Owner 华沃德源环境技术(济南)有限公司
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