Method for producing cow leather gelatin by using pickled raw leather

Abstract

The invention belongs to the technical field of gelatin extraction, and particularly relates to a method for producing cow leather gelatin by using pickled raw leather. The method is realized through the following steps: depilating cow leather, cleaning and degreasing; stirring and soaking the degreased cow leather in a gradient sodium chloride triethanolamine aqueous solution, and filtering and cleaning the cow leather after soaking; adding the treated cow leather into a phosphate buffer solution containing lauryl sodium sulfate, adding acid protease and sodium diethylhexyl sulfosuccinate, carrying out enzymolysis, and carrying out enzyme passivation after the enzymolysis is finished; and cleaning the cow leather subjected to enzymolysis to be neutral, oscillating the sol, filtering to obtain a glue solution, centrifuging the glue solution, collecting supernatant, and performing freeze drying. According to an extraction method provided by the invention, the gelatin extraction rate can be increased, and the extracted gelatin is high in purity, conforms to the related standards of medicinal cow leather gelatin and is uniform in particle size; according to the method provided by the invention, the enzymolysis time can be shortened, but the gelatin yield is not influenced.

Description

technical field [0001] The invention belongs to the technical field of gelatin extraction, and in particular relates to a method for producing cowhide gelatin by using pickled hides. Background technique [0002] Gelatin is a protein partially hydrolyzed from the collagen in the connective or epidermal tissue of animals. As a protein, gelatin has a special amino acid composition, with few sulfur-containing amino acids, and glycine, alanine, proline and The content of hydroxyproline is very high, accounting for 67% of the total amino acid content. Gelatin is almost colorless or light yellow in appearance, and is a transparent solid without special odor. It has excellent physical and chemical properties. Such as the formation of reversible gel, cohesiveness, surface activity, etc., it is widely used as jelly, emulsifier, stabilizer, adhesive, etc. in the food industry. [0003] The raw materials for the production of gelatin are mainly the skins, bones, tendons, etc. of vario...

AI Technical Summary

Problems solved by technology

At present, the extraction methods of gelatin mainly include: (1) gelatin is produced by hydrolyzing collagen by acid leaching. This method is suitable for producing gelatin with aggregate and pigskin. After acid treatment, the raw material is hard and the processing time is long; (2) Extract gelatin by pretreating collagen-containing raw materials with lime suspension, sodium hydroxide solution, etc. , is the method commonly used to produce gelatin by cowhide, but this method is easy to pollute the environment, and when the concentration of sodium hydroxide is not properly adjusted, it is easy to melt the collagen fibers and cause the raw materials to rot; (4) the collagen is dissolved by enzyme treatment and heated Denaturation and extraction of gelatin, this method has a short production cycle and less environmental pollution, but in the process of enzymatic hydrolysis and extraction, it is easy to cause partial dissolution of collagen, causing the enzymatic hydrolysis solution to become viscous, and the yield is low.

[0004] Chinese patent CN201811441992.6 provides a method for the production of cowhide gelatin by the alkaline method. The method is extracted four times after sodium sulfide treatment, lye treatment, etc., which improves the extraction efficiency. However, the method has a long period and a cumbersome process

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