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Method for producing tagatose from bionic silicon mineralized micro-capsule immobilized multienzyme

A technology of biomimetic silicon and tagatose, applied in the direction of immobilized enzymes, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the problems of high cost of protamine, unsuitable for industrial scale production, etc., and achieve Mild conditions, simple process, and reduced production costs

Active Publication Date: 2021-05-07
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(ShiJ , Jiang Z . An efficient and recyclable enzyme catalytic system constructed through the synergy between biomimetic mineralization and polyamine–saltaggregate assembly[J]. Journal of Materials Chemistry B, 2014, 2(28).) Although protamine can induce silicon Siliconized microcapsules are prepared from precursors, but protamine is expensive and not suitable for industrial scale production

Method used

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  • Method for producing tagatose from bionic silicon mineralized micro-capsule immobilized multienzyme
  • Method for producing tagatose from bionic silicon mineralized micro-capsule immobilized multienzyme
  • Method for producing tagatose from bionic silicon mineralized micro-capsule immobilized multienzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The biomimetic silicon mineralized microcapsule immobilized multi-enzyme of this example was prepared by the following method:

[0038] (1) Five main enzyme molecules involved in the production of tagatose: glucan phosphorylase, glucose phosphomutase, glucose phosphate isomerase, 6-phosphate tagatose 4-position epimerase and 6 Phosphotagatose phosphatase is pre-mixed according to the specific enzyme dosage, wherein the dosage of glucan phosphorylase is 0.1 mg / ml, the dosage of glucose phosphomutase is 0.1 mg / ml, the dosage of glucose phosphoisomerase The dosage of tagatose-6-phosphate 4-position epimerase was 0.2 mg / ml, and the dosage of tagatose-6-phosphate phosphatase was 0.2 mg / ml. Add the above-mentioned enzyme solution into the calcium chloride solution with a concentration of 0.33 M, and pour an equal molar concentration and equal volume of sodium carbonate solution into the calcium chloride solution at a speed of 700 r / min, react for 20-30 s, and Centrifuge at 3...

Embodiment 2

[0043] Compared with Example 1, the biomimetic silicon mineralization microcapsules immobilized multi-enzymes in this example differ only in that the number of layers of the biomimetic silicon mineralization microcapsules is changed from 1 layer to 2 layers. That is, the step (2) and step (3) of Example 1 are repeated twice, that is, after the calcium carbonate microspheres containing enzymes that are obtained in the step (2) of Example 1, the capsule wall layer is 1 layer, and then According to the mass ratio of 30:1, the polyethylenimine solution was uniformly mixed with the obtained calcium carbonate microspheres containing enzymes with one capsule wall layer for 10-20 minutes; centrifuged at a speed of 3000 r / min, After removing the supernatant, wash with deionized water until the supernatant does not contain polyethyleneimine; according to the mass ratio of 30:1, add the sodium silicate solution to the above microspheres and mix evenly for 10-20 min; at 3000 r Centrifuge ...

Embodiment 3

[0045] Compared with Example 1, the biomimetic silicon mineralization microcapsule immobilized multi-enzyme in this example differs only in that the number of layers of the wall of the biomimetic silicon mineralization microcapsule changes from 1 to 3 layers. That is, step (2) and step (3) of Example 1 are repeated 3 times, that is, the number of cyst wall layers obtained by repeating step (2) and step (3) in Example 2 is 2 layers of enzyme-containing After the calcium carbonate microspheres, finally according to the mass ratio of 30:1, the polyethyleneimine solution and the calcium carbonate microspheres containing the enzyme were uniformly mixed for 10-20 min; centrifuged at 3000 r / min to remove the above Wash the clear liquid with deionized water until the clear liquid does not contain polyethyleneimine; according to the mass ratio of 30:1, add the sodium silicate solution to the above microspheres and mix evenly for 10-20 min; at 3000 r / min Centrifuge at a high speed, remo...

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Abstract

The invention discloses a method for producing tagatose from bionic silicon mineralized micro-capsule immobilized multienzyme. The method comprises the step that the bionic silicon mineralized micro-capsule immobilized multienzyme and the prepared immobilized multienzyme are used for producing the tagatose. The method for preparing tagatose from immobilized multienzyme is simple and convenient in process and mild in condition, and has the advantages that the hollow core of the microcapsule not only can embed a large number of enzyme molecules, but also can endow the enzyme molecules embedded in the hollow core with a good physical and chemical microenvironment, and the stability of the enzyme molecules is enhanced; and the capsule wall of the micro-capsule has semi-permeability, so that the transfer and exchange of a substrate / product is realized, and the enzymatic reaction is facilitated. Particularly, when tagatose is prepared from immobilized multienzyme, the enzymes can be recycled. Compared with catalytic conversion of pure enzyme, the immobilized multienzyme can be recycled for multiple times, so that the use amount of the enzyme required for preparing tagatose for multiple times is greatly reduced, and the production cost is reduced.

Description

technical field [0001] The invention relates to the production field of tagatose, in particular to a method for producing tagatose by immobilizing multiple enzymes in biomimetic silicon mineralized microcapsules. Background technique [0002] Tagatose is a rare monosaccharide that occurs naturally and is the ketose form of galactose, the epimer of fructose. The sweetness of tagatose is similar to that of sucrose, and its calories are only one-third of that of sucrose, so it is called a low-calorie sweetener. Tagatose offers a very fresh and pure sweetness, with a taste profile similar to fructose. Studies have shown that tagatose has important physiological functional properties such as low calorie, low glycemic index, anti-caries, anti-oxidation, prebiotics, improvement of intestinal function, immune regulation, and drug precursors, and can be widely used in food, beverages, medicine, It has great economic value in fields such as health care (Oh D-K: Tagatose: properties,...

Claims

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Application Information

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IPC IPC(8): C12N11/18C12N11/14C12N11/089C12N11/04C12P19/24C12P19/18C12P19/16C12P19/02
CPCC12N11/18C12N11/14C12N11/089C12N11/04C12N15/52C12N9/1051C12N9/90C12N9/16C12Y503/01009C12Y204/01001C12Y504/02002C12Y501/03C12Y301/03C12P19/24C12P19/18C12P19/16C12P19/02C07K2319/00C07K2319/01C12N9/92C12N11/06C12N15/62C12N9/1205C12Y207/01144
Inventor 马延和石婷韩平平
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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