Extraction method and application of tamarind polysaccharide

Abstract

The invention provides an extraction method and application of tamarind polysaccharide, and the extraction method comprises the following steps: pretreating tamarind seeds to obtain tamarind gum powder, adding a trichloroacetic acid aqueous solution, performing stirring and reacting at 100-150 DEG C to obtain a tamarind polysaccharide mixed solution, carrying out solid-liquid separation, taking filtrate, performing concentrating, carrying out alcohol extraction, and performing drying to obtain the tamarind polysaccharide. According to the extraction method, the characteristics that tamarind polysaccharide is resistant to high temperature and not easy to degrade are utilized, on the basis of pretreatment, pressurized water extraction is performed under the ultra-high-temperature acidic condition, it is guaranteed that the tamarind polysaccharide can be fully dissolved out, and denaturation and precipitation of protein under the dual conditions of ultra-high temperature and trichloroacetic acid are promoted; the ultrafiltration concentration is adopted to further remove part of polypeptide protein, amino acid, inorganic salt and other small molecular impurities degraded at high temperature, and ethanol precipitation separation is combined to obtain high-purity tamarind polysaccharide; the method is simple in process, high in extraction efficiency, easy to amplify and suitable for batch production, and the protein content of the prepared tamarind polysaccharide is lower than 0.3 wt%.

Description

Technical field[0001]BACKGROUND OF THE INVENTION 1. Field of the Invention This invention relates to the field of extracting of plant polysaccharides, and more particularly to extraction methods of tamdam polysaccharide and its application thereof.Background technique[0002]Luo Haozi is a large treasure of Sukogu Acid Beans, also known as sour corners, sour beans; its seeds are approximately rectangular or oval, the outer skin is hard, reddish brown, including non-fibrous polysaccharide ingredients 60- 73 wt%, protein 15-20% by weight, fat and other impurities 8-13 wt%.[0003]In the 1960s, it was first realized the industrial production of Luodaozi polysaccharide by Japan, and as a food additive sales, then the United States, the United Kingdom, France, China and other countries have also begun the commercial production of Luo Haozi polysaccharide, which is in textile, Latex, architecture, wood, adhesive, explosives, paper, oil, and daily chemical industry have been widely used.[0004]...

AI Technical Summary

Problems solved by technology

However, the development and application of tamarind polysaccharides in China started relatively late, and there are few related research reports. It is only widely used in food production, and its deep-processed high value-added products are extremely scarce. High-purity tamarind polysaccharides There is no relevant domestic research report on the mass production preparation technology

[0005] The Chinese invention patent application with the publication number CN1034278A discloses a preparation method of tamarind gum, which is prepared by heating tamarind seed kernels, cooling them, grinding and decolorizing them, soaking in unleaded gasoline for degreasing, crushing, and sieving; the method The preparation process is simple and easy, the cost is low, and it can meet the requirements of tobacco sheet adhesives, but the purity of tamarind polysaccharide is not high, and the application range is narrow

[0006] The Chinese invention patent application with the publication number CN107501424A discloses a method for purifying tamarind polysaccharide gum, which mainly adopts the method of dispersing the calendered and broken tamarind polysaccharide gum in low-concentration ethanol-water solution to remove tamarind polysaccharide gum through enzymatic degradation. The purification method of protein in Wangzi polysaccharide gum improves product quality; this m