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Preparation method and application of multi-layer graphene oxide modified microbial carrier

A microbial carrier, fossil technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of reducing the adhesion speed and amount of microorganisms

Active Publication Date: 2021-06-04
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, commonly used microorganism carriers, such as polyurethane carriers, the hydrophobicity of its surface will directly reduce the adhesion speed and amount of microorganisms on its surface.

Method used

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  • Preparation method and application of multi-layer graphene oxide modified microbial carrier
  • Preparation method and application of multi-layer graphene oxide modified microbial carrier
  • Preparation method and application of multi-layer graphene oxide modified microbial carrier

Examples

Experimental program
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Effect test

preparation example Construction

[0051] In a first aspect, the invention provides a method for preparing a multilayer graphene oxide modified microbial carrier, such as figure 1 As shown, the method specifically includes the following steps:

[0052] Step 1 (S11), irradiating the first post-treated polyurethane carrier with an ultraviolet lamp to obtain a first intermediate product.

[0053] In this embodiment, the polyurethane carrier after the first post-treatment is clean and dry. During specific implementation, the clean and dry polyurethane carrier is spread under the ultraviolet lamp and irradiated for 30-60 minutes. During the irradiation process of the ultraviolet lamp, the amide bond in the polyurethane is broken, and the carboxylic acid group is formed to obtain the first intermediate product.

[0054] Optionally, in step 1 of this embodiment, the first post-processing includes:

[0055] Put the polyurethane sponge in an ultrasonic cleaner to vibrate and wash for 30-40 minutes, and then wash it wi...

Embodiment 1

[0070] Embodiment 1 (preparation of multilayer graphene oxide modified microbial carrier)

[0071] (1) Cut the polyurethane carrier into a sphere with a diameter of about 1 cm, put it into an ultrasonic cleaner and shake and wash it for 30 minutes, wash it with deionized water, and dry it in an oven to obtain a clean polyurethane carrier;

[0072] (2) Spread the clean and dry polyurethane carrier under the ultraviolet lamp and irradiate it for 60 minutes, the amide bond in the polyurethane is broken, forming a carboxylic acid group, and obtaining the first intermediate product;

[0073] (3) Prepare 6wt% ethanol solution of silane coupling agent KH550 (γ-aminopropyltriethoxysilane, APTES), and oscillate ultrasonically for 10 minutes to fully disperse the solution;

[0074] (4) Preparation of graphene oxide: Add 1.5g of graphite powder to 200mL of 9:1 sulfuric acid / phosphoric acid mixed solution, stir evenly and place in an ice-water bath; slowly add 9.0g of potassium permangana...

Embodiment 2

[0079] Embodiment 2 (immobilization of microorganisms)

[0080] The graphene oxide-modified polyurethane carrier prepared in Example 1 was used to conduct a biofilm-hanging test. The biological immobilization carrier was first soaked with nutrient solution, placed in a 500mL glass conical flask, and 200mL of nutrient solution was added to the bottle. Inoculate high-efficiency microbial preparations owned by Peking University with independent intellectual property rights, and add at 4g / L. Cultured in a constant temperature shaker, the temperature was set at 30°C.

[0081] On the 21st day of culture, Figure 5 Shows the SEM picture of the unmodified polyurethane carrier carrying out the film hanging culture test, Image 6 Shown is the SEM picture of the multilayer graphene oxide modified microbial carrier prepared in Embodiment 1 after the film-hanging culture test. like Figure 5 and 6 As shown, compared with the unmodified polyurethane carrier control, when cultured to t...

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Abstract

The invention provides a preparation method and application of a multi-layer graphene oxide modified microbial carrier. According to the method, a silane coupling agent is used for carrying out surface function modification on a polyurethane carrier, and multiple layers of graphene oxide are grafted on the surface of the carrier, so that the specific surface area and the surface structure of the carrier are increased, the microorganism surface attachment process is accelerated, and the biological loading capacity of the carrier is improved; moreover, hydrophilic functional groups are introduced into the polyurethane carrier through the graphene oxide, so that the hydrophilicity of the modified polyurethane biological carrier is improved, and the biocompatibility of the carrier is enhanced. Meanwhile, the preparation method of the multi-layer graphene oxide modified microbial carrier provided by the invention has the characteristics of simple process, low cost, strong thermal stability of the modified carrier and the like, and the prepared modified microbial carrier is beneficial to solving the problem of long biofilm culturing time in a biological immobilization process, the method has a good application prospect in a biological reaction sewage treatment system.

Description

technical field [0001] The invention relates to the field of sewage treatment, and mainly relates to a preparation method and application of a multilayer graphene oxide modified microbial carrier. Background technique [0002] With the continuous increase of sewage and wastewater discharge, water environmental problems have received more and more attention. The most commonly used sewage biological treatment methods are mainly activated sludge method and biofilm method. Among them, the biofilm method uses the surface of the carrier as a medium, and locates the free microbial cells on the surface of the carrier to form a biofilm mainly composed of microorganisms, so that it remains active and can be reused. It has a small amount of sludge discharge, It has obvious advantages such as strong impact resistance and low power consumption. [0003] Microbial carrier is the key factor of sewage biofilm treatment process, which plays a vital role in the treatment effect of biologica...

Claims

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Application Information

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IPC IPC(8): C02F3/34C08J3/28C08L75/04
CPCC02F3/34C08J3/28C08J2375/04
Inventor 籍国东王亚芹
Owner PEKING UNIV
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