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Composite bifidobacterium preparation capable of resisting allergy, enhancing immunity, reducing blood sugar, reducing fat and losing weight and preparation method of composite bifidobacterium preparation

A compound bifidobacteria, lipid-lowering and weight loss technology, applied in the directions of bifidobacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low concentration of viable bacteria, poor fermentation effect, low bacterial activity, etc. To achieve the effect of increasing the number of bacteria, increasing vitality, and reducing drying stress

Pending Publication Date: 2021-06-22
盐城维康生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But because the solid content in the liquid fermented liquid is very low (2-5%), so even if the added liquid culture liquid consumption reaches 50%, also can only add a small amount of liquid bacterial classification (1-2.5%) in the solid medium As a solid-state fermentation strain, the initial bacterial concentration in the solid-state fermentation medium is not high, resulting in a long solid-state fermentation time and a small concentration of viable bacteria in the product
[0011] To sum up, whether it is the traditional actual production process of probiotics "liquid culture + centrifugation" or the above-mentioned "liquid culture + solid state culture" process of the invention, there are low strain content, poor fermentation effect, and bacterial growth when dry. Defects such as high mortality rate and low bacterial viability in the finished product

Method used

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  • Composite bifidobacterium preparation capable of resisting allergy, enhancing immunity, reducing blood sugar, reducing fat and losing weight and preparation method of composite bifidobacterium preparation
  • Composite bifidobacterium preparation capable of resisting allergy, enhancing immunity, reducing blood sugar, reducing fat and losing weight and preparation method of composite bifidobacterium preparation
  • Composite bifidobacterium preparation capable of resisting allergy, enhancing immunity, reducing blood sugar, reducing fat and losing weight and preparation method of composite bifidobacterium preparation

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0058] The preparation method includes the following steps:

[0059] (1) Mix Bifidobacterium bifidum Miuyo-01, Bifidobacterium infantis Miuyo-21, Bifidobacterium breve Miuyo-31 and Bifidobacterium lactis Miuyo-11 according to the ratio to form a compound bifidobacterium, and put it into the liquid The first anaerobic culture is carried out in the culture medium, and the culture solution produced is centrifuged to obtain the wet cells of bifidobacteria, which are set aside;

[0060] (2) Mix the wet thalline of the compound bifidobacterium obtained in step (1) with the solid medium, carry out anaerobic cultivation for the second time in a sealed state, carry out low-temperature drying after the cultivation is completed, and form a solid compound bifidobacterium ;

[0061] (3) The composite bifidobacteria solids obtained in step (2) are pulverized, anaerobically granulated, and anaerobically coated to obtain the composite bifidobacterium preparations for anti-allergy, increased ...

Embodiment 1

[0065] Isolation, strain characteristics and identification of embodiment 1 bacterial strain

[0066] 1.1 Sample collection:

[0067] Select healthy infants who were 3 months to 1 year after birth and have not used antibacterial drugs recently, and collect samples from their feces for isolation, that is, the isolated strains come from the intestinal tract of healthy infants.

[0068] 1.2 Isolation of strains

[0069] Take 5-10g of infant feces samples, put them in a sterile 50ml centrifuge tube, add 20ml of sterile normal saline, shake and mix well, put them in an anaerobic incubator at 37°C, let them stand for overnight culture, draw 1ml of the sample solution, and Sterile normal saline was sequentially diluted 10 times to 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 For each gradient, 100 μl of bacterial suspension was coated on the improved MRS plate, and placed in an anaerobic incubator at 37°C for upside-down culture. After 24 hours, colonies were selected from th...

Embodiment 2

[0090] The characteristic of embodiment 2 bacterial strains

[0091] 2.1 Determination of the number of live bacteria in the product

[0092] After gradient dilution with sterilized physiological saline to a certain multiple, adopt the improved MRS agar medium plate pouring method, and count the total number of colonies after culturing at 37°C for 48 hours.

[0093] 2.2 Growth at different pH

[0094] Inoculate the above-mentioned 5 activated strains in the improved MRS liquid medium, obtain the seed liquid of the strain after cultivation, take 1 mL of the seed liquid and inoculate in 19 mL of the improved MRS liquid medium with pHs of 6.0, 6.5, 7.0 and 7.5 respectively , anaerobic culture at 37°C for 24h, measure the initial and end OD600 values ​​(that is, the absorbance value at 600nm wavelength, which can usually be used to compare the cell density or growth of the bacteria in the culture solution), and the pH7 The OD600 value of 0.0 is used as a contrast (that is, the num...

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Abstract

The invention relates to a composite bifidobacterium preparation capable of resisting allergy, enhancing immunity, reducing blood sugar, reducing fat and losing weight as well as a preparation method and application of the composite bifidobacterium preparation. Bifidobacterium bifidum Miuyo-01, bifidobacterium infantis Miuyo-21, bifidobacterium breve Miuyo-31 and bifidobacterium lactis Miuyo-11 are adopted for preparing the composite bifidobacterium preparation. The composite bifidobacterium preparation is prepared by a novel process combining mixed bacteria inoculation, anaerobic liquid culture, solid-liquid separation, solid culture and the like, and the produced composite bifidobacterium preparation is large in thallus quantity and high in activity and contains various metabolites such as alpha-glucosidase inhibitors, bacterial polysaccharides, peptidoglycan and lipoteichoic acid produced by the composite bifidobacterium; better effects of resisting allergy, enhancing immunity, reducing blood sugar, reducing fat and losing weight are achieved; in the preparation process, liquid anaerobic culture is adopted, and a generated culture solution is centrifugally separated to obtain wet thalli of the composite bifidobacterium; mixing the wet thalli with a sterile solid culture medium, and carrying out secondary solid anaerobic culture in a sealed state; and after the solid culture is completed, performing low-temperature drying to obtain the high-activity composite bifidobacterium solid.

Description

technical field [0001] The invention belongs to the technical field of probiotic preparations, and in particular relates to a compound bifidobacterium preparation for anti-allergic, immune-enhancing, blood-sugar-lowering, lipid-lowering and weight-reducing properties and a preparation method thereof. Background technique [0002] Allergy is a phenomenon in which an organism's sensitivity to certain external stimuli (antigens) is abnormally increased, that is, an abnormal, excessively high immune response, simply speaking, it is an oversensitivity to a certain substance (antigen). The occurrence and development of allergic diseases are related to the body's immune system, and the type 2 immune response mechanism plays an important role in allergic diseases, which can produce specific IgE antibodies against allergens and make the body in a sensitized state. When the sensitized body is exposed to the same allergen again, the IgE antibody adsorbed on the surface of mast cells an...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/745A61P37/08A61P37/04A61P3/10A61P3/06A61P3/04A61P1/00C12R1/01
CPCC12N1/20A23L33/135A61K35/745A61P37/08A61P37/04A61P3/10A61P3/06A61P3/04A61P1/00A23V2002/00A23V2400/529A23V2400/517A23V2400/519A23V2400/531A61K2300/00A23V2200/304A23V2200/324A23V2200/328A23V2200/332A23V2200/3204Y02A50/30
Inventor 陈大伟童颖佳童群义陈金林吉峰
Owner 盐城维康生物科技有限公司
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