Method for removing free fatty acid in grease by enzyme catalysis

A free fatty acid removal technology, applied in the direction of fat oil/fat refining, fat production, etc., can solve the problems of reducing enzyme catalytic activity and stability, increasing the cost of decolorization treatment, loss of active components of oil, etc., to achieve neutral oil loss Small size, good economy and environmental protection, and the effect of improving the oil yield

Active Publication Date: 2021-07-02
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Claims
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Problems solved by technology

Among them, chemical alkali refining deacidification is the most commonly used method because of its low cost, simple process flow and high deacidification efficiency, but the disadvantage of this process is that it will form a large amount of saponins, especially the high acid value crude oil leads to neutral oil The loss is large, and the active ingredients in the oil are also lost due to the saponin, which reduces the quality of the oil, and a large amount of industrial wastewater will be generated in the subsequent process of chemical deacidification, which will aggravate the environmental pressure
The physical deacidification method also has a certain application prospect. Its main characteristics are simple process flow and no waste water pollution, but the energy consumption demand is large, the process temperature is high, and it is easy to cause the occurrence of oil side reactions. Deep color increases the cost of subsequent decolorization treatment
In comparison, the enzymatic deacidification of biological lipase has attracted more and more attention, mainly due to the advantages of green, safe, and specificity of the enzymatic deacidification process, although the enzymatic deacidification process has relatively high Good application potential, its traditional process is still insufficient, usually enzymatic catalytic deacidification needs to add strong polar acyl acceptors (polar alcohols) to react with free fatty acids, catalyze the formation of product glyceride and water, and the reaction It is reversible. In order to speed up the forward reaction, the strategy is to maintain a vacuum to reduce the accumulation of product moisture, but this process undoubtedly limits the batch addition of volatile acyl acceptors, and the "one-pot" process is directly affected by the high concentration of polar alcohols. Reduce enzyme catalytic activity and stability, it is difficult to meet the requirements of actual industrial production

Method used

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  • Method for removing free fatty acid in grease by enzyme catalysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 100 g of choline chloride / glycerol natural deep eutectic solvent with a molar ratio of 1:3 and add it to the reactor, add 100 g of rice bran crude oil, soybean crude oil and rapeseed crude oil to the above prepared Add 10g of Lipozyme TL IM lipase to the solvent, stir the mixture in a water bath at 30°C for 3 hours, centrifuge and take the upper layer of oil for acid value analysis, the acid value of rice bran crude oil is 2.47mg KOH / g, the deacidification rate is 92.78%, and the neutral oil The yield is 97.23%, the measured acid value of soybean crude oil is 0.28mg KOH / g, the deacidification rate is 89.86%, the yield of neutral oil is 98.17%, the measured acid value of rapeseed crude oil is 0.26mg KOH / g, the deacidification rate is 90.91 %, neutral oil yield 96.34%. The deacidification efficiency of the system with continuous recovery of 10 batches of solvent oil and lipase in the lower layer did not decrease significantly.

Embodiment 2

[0032] Weigh 100g of choline chloride / n-butanol natural deep eutectic solvent with a molar ratio of 1:2 and add it to the reactor, and add 50g of rice bran crude oil, soybean crude oil and rapeseed crude oil to the above-mentioned Add 10g Lipozyme RM IM lipase to the prepared solvent, stir the mixture in a water bath at 40°C for 1 hour, centrifuge and take the upper layer of oil for acid value analysis, the acid value of rice bran crude oil is 2.78 mg KOH / g, and the deacidification rate is 91.87%. The yield of neutral oil is 96.27%, the measured acid value of soybean crude oil is 0.27mg KOH / g, the deacidification rate is 90.22%, the yield of neutral oil is 94.23%, the measured acid value of rapeseed crude oil is 0.31mg KOH / g, and the deacidification rate is 90.22%. The yield was 89.16%, and the yield of neutral oil was 80.32%. The deacidification efficiency of the system with continuous recovery of 10 batches of solvent oil and lipase in the lower layer did not decrease signif...

Embodiment 3

[0034] Weigh 100 g of choline chloride / tert-butanol natural deep eutectic solvent with a molar ratio of 1:1 and add it to the reactor, and add 200 g of rice bran crude oil, soybean crude oil and rapeseed crude oil to the above-mentioned Add 10g of Novozyme 435 lipase to the prepared solvent, stir the mixture in a water bath at 50°C for 0.5h, centrifuge and take the upper layer of oil for acid value analysis, the acid value of rice bran crude oil is 4.45mg KOH / g, and the deacidification rate is 86.99%. The yield of neutral oil is 93.16%, the measured acid value of soybean crude oil is 0.24mg KOH / g, the deacidification rate is 91.30%, the yield of neutral oil is 95.31%, the measured acid value of rapeseed crude oil is 0.26mg KOH / g, and the deacidification rate is 91.30%. The yield is 90.91%, and the yield of neutral oil is 95.42%. The deacidification efficiency of the 8-batch system of continuous recovery of lower solvent oil and lipase did not decrease significantly.

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Abstract

The invention discloses a method for removing free fatty acid in grease by enzyme catalysis, which comprises the following steps of mixing a natural deep-eutectic solvent with crude vegetable oil, adding lipase, stirring for reaction, and after the reaction is finished, performing centrifugal separation to obtain an upper oil phase, namely the crude vegetable oil without the free fatty acid. The multifunctional natural deep-eutectic solvent system constructed by the invention can realize synchronous deacidification and dehydration of lipase catalysis, the acyl acceptor type natural deep-eutectic solvent is a reaction substrate and a reaction medium, and is esterified with free fatty acid as a substrate into glyceride, so that the conversion of the free fatty acid into the glyceride is realized, the raw material loss is reduced, and the grease yield is improved. Quick separation from an oil phase can be achieved, the deacidification process is accelerated, the reaction time can be shortened to be within 3h, the product is easy to separate and purify, and a catalytic reaction system can be recycled in multiple batches.

Description

technical field [0001] The invention relates to a processing technology for oil refining and deacidification, in particular to a method for using a multifunctional natural deep eutectic solvent to catalyze the removal of free fatty acids in oil by lipase. Background technique [0002] Unprocessed natural animal and vegetable oils usually contain nutrient-rich glycerides, active components and proteins, as well as substances such as easily oxidized free fatty acids. The existence of such substances will accelerate the oxidation of oils and even threaten human health, thereby It is necessary to process and remove free fatty acids to make edible oil meet the national food safety standards. Therefore, processing and deacidification is an essential process in the oil refining process. [0003] The current deacidification processes for oil mainly include chemical alkali refining deacidification, physical deacidification, and biological enzymatic deacidification. Among them, chemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00
CPCC11B3/003
Inventor 周鹏飞张名位邓媛元张雁赵志浩魏振承唐小俊刘光李萍
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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