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Patterned bacterial cellulose composite membrane with biological activity as well as preparation and application thereof

A technology of bacterial cellulose film and bacterial cellulose, which can be used in viscous dressings, bandages, dressings, etc., and can solve the problems of scar formation and uncontrolled collagen of fibroblasts

Pending Publication Date: 2021-07-06
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such skin repair dressings can lead to uncontrolled proliferation of fibroblasts leading to excessive collagen deposition, which can lead to tissue fibrosis and scarring (Keane T.J., Horejs C., Stevens M.M., Adv. Drug Deliv .Rev.,2018,129:407-419; Coentro J.Q.,Pugliese E.,Hanley G.,Raghunath M.,Zeugolis D.I.,Adv.Drug Deliv.Rev.,2019,146:37-59.)

Method used

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  • Patterned bacterial cellulose composite membrane with biological activity as well as preparation and application thereof
  • Patterned bacterial cellulose composite membrane with biological activity as well as preparation and application thereof
  • Patterned bacterial cellulose composite membrane with biological activity as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Pattern design and preparation of polydimethylsiloxane PDMS template in the present invention, comprise the following steps:

[0051] (1) Use AutoCAD drawing software to design a uniform stripe groove pattern with a width of 10 μm, or a stripe groove pattern with a width of 10 μm and 5 μm alternately arranged. The designed pattern is photolithographically etched on the silicon wafer, and then the surface is silanized by perfluorosilane to enhance the inertia of the surface of the patterned template and prolong the service life;

[0052] (2) Fully mix the two liquids of polydimethylsiloxane (PDMS) and curing agent according to the mass ratio of 10:1, remove the air bubbles, and obtain a transparent viscous liquid; pour the PDMS with the air bubbles removed on the patterned light On the engraving template, the thickness is 3mm, and the air bubbles are further removed by vacuuming;

[0053] (3) Put the template in an oven to completely cure the PDMS, then carefully peel o...

Embodiment 2

[0056] This embodiment provides a method for preparing a patterned BC membrane, specifically: preparing Hestrin&Schramm medium, and the components and ratios are: citric acid (0.15%, w / v), disodium phosphate (0.27%, w / v), yeast extract (0.5%, w / v), peptone (0.5%, w / v) and glucose (2%, w / v). Dissolve each component in deionized water, inoculate Acetobacter xylinum in the sterilized medium after high temperature sterilization, place the PDMS template with a stripe width of 10 μm upside down in the medium, and incubate at 30°C Cultured for 7 days. Afterwards, boil with 1M NaOH solution to remove residual bacteria in the BC membrane, and then wash with deionized water several times until the pH is neutral. Figure 5 It is the FESEM image of the prepared mBC film, from which it can be seen that the mBC is oriented and arranged as a relatively dense and neatly arranged fiber groove structure.

Embodiment 3

[0058] Prepare Hestrin&Schramm medium, each component and proportion are: citric acid (0.15%, w / v), disodium phosphate (0.27%, w / v), yeast extract (0.5%, w / v), peptone (0.5% , w / v) and glucose (2%, w / v). Each component was dissolved in deionized water, and after high-temperature sterilization, Acetobacter xylinum was inoculated in the sterilized medium, and the striped groove PDMS template with a width of 10 μm and 5 μm was placed upside down in the medium. Cultured in a 30°C incubator for 7 days. Afterwards, boil with 1M NaOH solution to remove the remaining bacteria in the BC film, and then wash it with deionized water for several times until its pH is neutral, and then obtain a film with different widths of staggered fiber groove structures.

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Abstract

The invention discloses a patterned bacterial cellulose composite membrane with biological activity as well as preparation and application thereof, and relates to the technical field of biomedical polymer composite materials. The preparation method comprises the following steps: inversely placing a template with a pattern structure in a bacterial culture solution capable of synthesizing bacterial cellulose, and culturing for a period of time to obtain a bacterial cellulose membrane with a pattern structure; and then dipping into a natural polymer active material solution, so that the bacterial cellulose with the pattern structure and the natural polymer active material are compounded through hydrogen bond acting force, to obtain the patterned bacterial cellulose composite membrane with biological activity. The composite membrane can rapidly induce skin tissue regeneration and reduce scar formation, has a simple preparation method, is green and environmentally friendly, can be produced on a large scale, and has a good application prospect in skin injury repair.

Description

technical field [0001] The invention relates to the technical field of biomedical polymer composite materials, in particular to a bioactive patterned bacterial cellulose composite film and its preparation and application for preparing wound dressings. Background technique [0002] Since skin damage will expose the body to various harmful substances and even cause serious blood loss, in order to quickly induce the skin to restore its protective function, some researchers have designed various wound dressings to restore the physical barrier function of the skin, and Promotes wound healing by accelerating skin tissue regeneration. However, wound dressings face the challenge of restoring the structural integrity of the skin's natural tissue. Some of these dressings lack good bioactivity and cannot accelerate wound healing, while others are able to induce wound healing in a relatively short period of time. This will cause the damaged skin to fail to return to its original norma...

Claims

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Application Information

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IPC IPC(8): A61F13/02A61L15/32A61L15/28A61L15/42
CPCA61F13/0259A61F13/0289A61F13/00063A61L15/32A61L15/325A61L15/28A61L15/42C08L1/02A61L15/40A61L2400/18C08L5/08C08J5/18A61L15/22A61L15/44C08J7/04
Inventor 杨光白鸥李晓宏兰博妮
Owner HUAZHONG UNIV OF SCI & TECH
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