Patterned bacterial cellulose composite membrane with biological activity as well as preparation and application thereof

A technology of bacterial cellulose film and bacterial cellulose, which can be used in viscous dressings, bandages, dressings, etc., and can solve the problems of scar formation and uncontrolled collagen of fibroblasts

Pending Publication Date: 2021-07-06

HUAZHONG UNIV OF SCI & TECH

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AI-Extracted Technical Summary

Problems solved by technology

However, such skin repair dressings can lead to uncontrolled proliferation of fibroblasts leading to excessive collagen deposition, which can lead to tissue fibrosis and scarring...

Method used

[0051] (1) adopt AutoCAD drawing software to design a uniform striped groove pattern with a width of 10 μm, or a staggered striped groove pattern with a width of 10 μm and 5 μm. The designed pattern ...

Abstract

The invention discloses a patterned bacterial cellulose composite membrane with biological activity as well as preparation and application thereof, and relates to the technical field of biomedical polymer composite materials. The preparation method comprises the following steps: inversely placing a template with a pattern structure in a bacterial culture solution capable of synthesizing bacterial cellulose, and culturing for a period of time to obtain a bacterial cellulose membrane with a pattern structure; and then dipping into a natural polymer active material solution, so that the bacterial cellulose with the pattern structure and the natural polymer active material are compounded through hydrogen bond acting force, to obtain the patterned bacterial cellulose composite membrane with biological activity. The composite membrane can rapidly induce skin tissue regeneration and reduce scar formation, has a simple preparation method, is green and environmentally friendly, can be produced on a large scale, and has a good application prospect in skin injury repair.

Application Domain

Adhesive dressingsAbsorbent pads +1

Technology Topic

Biological organismMacromolecule +11

Image

Examples

Experimental program(11)
Comparison scheme(2)

Example Embodiment

[0049] Example 1

[0050] The patterned design and preparation of polydimethylsiloxane PDMS template, including the following steps:

[0051] (1) The use of a uniform striped groove pattern having a width of 10 μm using the AutoCAD drawing software, or a striped groove pattern of a width of 10 μm and 5 μm interlaced. The design is photolitholized on the silicon wafer, and then the silane treatment of the surface of the patterning template is inert to the surface of the patterned template to extend the service life;

[0052] (2) Two liquids of polydimethylsiloxane (PDMS) and curing agent are thoroughly mixed in mass than 10: 1, remove bubbles to obtain transparent viscous liquid; pouring the PDMS removing bubbles in patterned light On the expansion template, the thickness is 3 mm, and the bubbles are further removed by the vacuum method;

[0053] (3) Place the template to completely cure the PDMs, and then take PDMS from the patterned photoresist template, PDMS form a pattern that is complementary to the template, and cuts it to meet the required dimensions.

[0054] Figure 4 It is an optical microscope diagram of a uniform width trench (10 μm) PDMS template, which is arranged in parallel from the visible trench striped structure, and each stripe width is consistent.

Example Embodiment

[0055] Example 2

[0056] This embodiment provides a method of preparing a patterned BC film, specifically: formulated HEStrin & Schramm medium, each component and ratio: citric acid (0.15%, w / v), sodium phosphate (0.27%, w / v), yeast cream (0.5%, w / v), protein (0.5%, w / v) and glucose (2%, w / v). Each component is dissolved in deionized water, and the pneumacteriococci is inoculated in a sterilized medium, and the PDMS template of the striped width is 10 μm is inverted in the medium, in an incubator at 30 ° C. 7 days were cultured. The bacterium residual in the BC film was boiled with 1 M NaOH solution, and then washed multiple times with deionized water until its pH was neutral. Figure 5 It is the prepiem diagram of the prepared MBC film, which can be seen from the figure, the MBC is arranged as a relatively dense and arranged fiber trench structure.

Example Embodiment

[0057] Example 3

[0058] Hestrin & Schramm medium, each component and ratio are: citric acid (0.15%, w / v), sodium phosphate (0.27%, w / v), yeast cream (0.5%, w / v), protein (0.5%) , W / v) and glucose (2%, w / v). Each component was dissolved in deionized water, and the wood acette was inoculated in a sterilized medium, respectively, in a sterilized medium, inverted the striped groove PDMS template of the width of 10 μm and 5 μm, in the medium, The incubator was cultured for 7 days in an incubator at 30 ° C. The bacterium residual in the BC film was boiled with 1 M NaOH solution, and then washed multiple times with deionized water until its pH is neutral, i.e., a fiber trench structure film that is arranged in different width interleaves.

PUM

Property	Measurement	Unit
Quality score	0.0 ~ 0.03

Description & Claims & Application Information

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