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Controlled-release antibacterial composite hydrogel as well as preparation method and application thereof

A composite hydrogel and hydrogel technology, which can be used in capsule delivery, pharmaceutical formulations, bandages, etc., can solve the problems of non-adhesive cells, poor mechanical strength, and hindering applications

Active Publication Date: 2021-07-06
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, HA has poor mechanical strength and is not sticky to cells, which hinders its application in the biomedical field.

Method used

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  • Controlled-release antibacterial composite hydrogel as well as preparation method and application thereof
  • Controlled-release antibacterial composite hydrogel as well as preparation method and application thereof
  • Controlled-release antibacterial composite hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of metal polyphenol capsule (Cu-EGCG), the steps are as follows:

[0037] First prepare CaCl at a concentration of 20 mM 2 stock solution and Na 2 CO 3 Stock solution, PSS was dissolved in the above two stock solutions at a concentration of 1 mg / mL. With vigorous stirring, 10 mL of Na 2 CO 3 Quickly add the stock solution to 10 mL containing CaCl 2The stock solution was stirred continuously for 30 seconds, then the solid particles were collected by centrifugation and washed 3 times with deionized water to obtain CaCO 3 particles.

[0038] To the 16 mg CaCO prepared above 3 Add 5 mg EGCG and 0.5 mL of 24 mM CuCl to the pellet 2 aqueous solution to obtain a slurry, vortex the slurry for 10 s, and then add 5 mL of MOPS buffer solution (100 mM, pH 8.0) to wash off excess EGCG and CuCl 2 , after filtration to obtain CaCO coated with a film layer on the surface 3 Particles, the film layer is obtained by forming a Cu-EGCG coordination network b...

Embodiment 2

[0041] The preparation method of methacrylated hyaluronic acid (HAMA), the steps are as follows:

[0042] Weigh 2g of hyaluronic acid and dissolve it in 100mL of distilled water. After the hyaluronic acid is completely dissolved, add 1.6mL of methacrylic anhydride, and then adjust the pH of the reaction system to 8.5 with a sodium hydroxide solution with a concentration of 1mol / L. Then react at room temperature for 8 hours, dialyze with distilled water for 3 to 5 days in a dialysis bag (molecular weight cut-off: 3500Da), and finally freeze-dry to obtain HAMA.

[0043] In fact, the amount of methacrylic anhydride added in the above preparation method can be 1-3mL, the pH value of the reaction system can be controlled at 8-9, and the reaction time can be 4-12h. Under the above conditions, the performance of the present invention can be satisfied. Require.

Embodiment 3

[0045] The preparation method of methacrylate modified silk fibroin (Sil-MA), the steps are as follows:

[0046] Put the cocoon from which the silkworm chrysalis has been removed into 1L of 0.05M Na 2 CO 3 In the solution, boil at 100°C for 20 minutes, repeat 3 times to completely remove sericin, then wash with distilled water several times, and dry at 60°C for the next step.

[0047] 20 g of the degummed cocoons were taken and dissolved in 100 mL of 9.3 M lithium bromide (LiBr) solution at 60° C. for 1 h. After dissolving silk fibroin (SF) with lithium bromide, 12 mL of glycidyl methacrylate (GMA) was immediately introduced into the mixed solution, reacted at 300 rpm at 60 °C for 6 h, and then centrifuged the resulting solution at 8000 r / min for 5 min , take the supernatant, filter it with a magic filter cloth, dialyze it with distilled water in a dialysis bag (molecular weight cut-off: 12-14kDa) for 3-5 days, and finally freeze-dry to obtain Sil-MA.

[0048] In fact, when...

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Abstract

The invention discloses controlled-release antibacterial composite hydrogel. The controlled-release antibacterial composite hydrogel comprises a hydrogel matrix and a metal polyphenol capsule loaded in the hydrogel matrix, wherein the hydrogel matrix is formed by crosslinking methacrylic acid modified hyaluronic acid and metacrylic acid ester modified silk fibroin, and the metal polyphenol capsule is prepared from the following components: epigallocatechin-3-gallate and copper ions. According to the invention, EGCG and copper ions form a Cu-EGCG coordination network, the metal polyphenol capsule is prepared and loaded into the hydrogel, so that continuous slow release of EGCG and copper ions is realized, wound inflammation is inhibited while wound angiogenesis is promoted, the bioavailability of EGCG is improved, the toxicity of copper ions is reduced, and healing of chronic wounds is facilitated. Meanwhile, the hydrogel with a double-network structure can be formed through composite cross-linking of HAMA and Sil-MA, so that the hydrogel has good biocompatibility, keeps the original performance of a polymer, has better mechanical performance and better meets the application requirements of wound dressings.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a controlled-release antibacterial composite hydrogel and its preparation method and application. Background technique [0002] The skin plays a vital role in maintaining homeostasis and protecting against invading microorganisms and chemicals, serving as the body's first line of defense against injury, infection and dehydration. However, skin with limited ability to heal is also highly vulnerable to trauma such as burns and bruises. Usually, the preferred option for skin regeneration is autologous skin grafting, but the shortage of donor skin and the inevitable new wounds at the donor site limit its widespread application. Therefore, today, wound dressings have become a mainstream therapy for skin repair in response to the high annual incidence of skin damage. [0003] A good-performing wound dressing should contain ingredients that promote tissue rege...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00C08F299/00C08F2/48C08F2/44
CPCA61L26/0047A61L26/0023A61L26/0066A61L26/008A61L26/0004C08F299/00C08F2/48C08F2/44A61L2300/216A61L2300/102A61L2300/404A61L2300/412A61L2300/602A61L2300/62C08L89/00C08L5/08
Inventor 郭瑞胡圣学冯龙宝
Owner BEOGENE BIOTECH GUANGZHOU
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