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Bacillus subtilis with capability of efficiently degrading gossypol and application thereof

A technology of Bacillus subtilis and Lactobacillus plantarum, applied in the field of microbial technology and feed application, to achieve the effects of reducing the death rate, improving the palatability and digestibility of feed, and improving the production performance of broilers

Inactive Publication Date: 2021-07-23
NEW HOPE LIUHE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems in the application of microbial detoxification method, such as the stability of the detoxification effect of microorganisms in large-scale solid-state fermentation of cottonseed meal, the safety of strains, and the combination fermentation interaction between gossypol detoxification strains and other functional strains. Further research is needed on issues such as the relationship between

Method used

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  • Bacillus subtilis with capability of efficiently degrading gossypol and application thereof
  • Bacillus subtilis with capability of efficiently degrading gossypol and application thereof
  • Bacillus subtilis with capability of efficiently degrading gossypol and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Isolation, screening and identification of embodiment 1 bacterial strain

[0022] 1 Materials and methods

[0023] 1.1 Test material

[0024] 1.1.1 Samples come from cotton field soil.

[0025] 1.1.2 Medium

[0026] Enrichment medium: 1% cotton meal, 1% glucose, 1% sucrose, 0.1% magnesium sulfate, 0.1% potassium dihydrogen phosphate, natural pH, sterilized at 121°C for 20 minutes.

[0027] Primary screening medium: ammonium sulfate 5g, potassium dihydrogen phosphate 1g, sodium chloride 0.1g, magnesium sulfate 0.5g, calcium chloride 0.1g, yeast extract 0.2g, agar 20g, distilled water 1000ml, 0.1% or 0.2% cotton acetate Phenol (pre-dissolved in acetone).

[0028] Re-screening medium: according to different strains, select potato dextrose water, nutrient broth, MRS broth medium, add gossypol acetate solution (25mg / ml) to it to make the final concentration 500mg / L.

[0029] 1.2 Test method

[0030] 1.2.1 Enrichment of gossypol-degrading bacteria

[0031] Weigh 10 g o...

Embodiment 2

[0052] Example 2 Strain YB-10 solid-state fermentation of cotton meal

[0053] 1 Materials and methods

[0054] 1.1 Test material

[0055] Cotton meal: Xinjiang cotton meal.

[0056] Bacteria: the YB-10 bacteria screened in Example 1.

[0057] 1.2 Medium

[0058] Seed medium: Nutrient broth medium.

[0059] Solid-state fermentation medium: cottonseed meal 100g, glucose 2g, tap water 40ml.

[0060] 1.3 Test method

[0061] The screened test strains were inoculated into the liquid seed medium, and cultured on a shaker at 160 rpm for 20 hours at 37°C. Then add the seed solution into the solid-state fermentation medium according to the amount of 10% of the mass of cotton meal, put it into a sealed bag, mix it evenly, expose it, and cultivate it at 30°C for 48 hours, and turn over the material every 12 hours. After the fermentation, the fermented cottonseed meal was placed in an oven at 65°C and dried for 4-6 hours to make an air-dried sample, which was crushed through 40 me...

Embodiment 3

[0081] Embodiment 3 mixed bacteria solid-state fermentation cotton meal

[0082] 1 Materials and methods

[0083] 1.1 Test material

[0084] Cotton meal: Xinjiang cotton meal.

[0085] Bacillus subtilis: the YB-10 bacteria screened in Example 1.

[0086] Saccharomyces cerevisiae: JM-4, JM-6.

[0087] Lactic acid bacteria: Lactobacillus plantarum RS-1, Enterococcus faecium RS-6.

[0088] 1.2 Medium

[0089] Seed culture medium: nutrient broth, for cultivating Bacillus subtilis; potato dextrose water, for cultivating Saccharomyces cerevisiae; MRS broth, for cultivating lactic acid bacteria.

[0090] Solid-state fermentation medium: cottonseed meal 100g, glucose 2g, tap water 40ml.

[0091] 1.3 Test method and test design

[0092] The gossypol-degrading bacteria YB-10 is used as the main fermentation bacteria, combined with yeast and lactic acid bacteria for fermentation. The test strains were inoculated into the corresponding liquid seed medium, and cultured on a shaker ...

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Abstract

The invention discloses bacillus subtilis capable of efficiently degrading gossypol and application thereof, the bacillus subtilis is classified and named as bacillus subtilis and is preserved in China General Microbiological Culture Collection Center on November 4, 2020, the preservation number is CGMCC No.21103, and the preservation address is Beijing in China. The bacillus subtilis is a microbial strain allowed to be used in the catalog of varieties of feed additives (2013) and can grow by taking gossypol acetate as a sole carbon source, the gossypol degradation rate by liquid fermentation is as high as 94%, the gossypol degradation rate of cottonseed meal by single-bacterium solid-state fermentation is 90.7%, by the combined fermentation of the bacillus subtilis, yeast and lactic acid bacteria with the gossypol degradation as the main function, the degradation rate of the cottonseed meal is higher than 90%, and the crude protein content, the acid-soluble protein content and the total acid content are further increased, so that the bacillus subtilis can be effectively applied to livestock and poultry feeds, particularly to broiler feed, the palatability and the digestibility of the feeds are improved, the broiler production performance is improved, and the death and culling rate is reduced.

Description

technical field [0001] The invention relates to a bacillus subtilis strain capable of efficiently degrading gossypol and an application thereof, belonging to the field of microbial technology and feed application. Background technique [0002] my country is rich in cotton meal resources. In recent years, the average output of cotton is about 6 million tons / year, and the output of cottonseed cake (meal) is about 4 million tons / year. [0003] Cotton meal is a very good unconventional protein feed resource, with a crude protein content of more than 40%, and the crude protein content of dehulled cottonseed meal can reach more than 50%. Cottonseed meal is rich in amino acids and is an excellent source of tryptophan and methionine in feed, as well as vitamin E and B vitamins. [0004] However, there are still defects in the application of cotton meal in livestock and poultry feed, mainly because of the toxic substance free gossypol contained in cotton meal. Free gossypol can cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/75A23K10/12C12R1/125
CPCA23K50/75A23K10/12
Inventor 张遨然周航尹望周桂莲燕磊蔡姬姮王红梅李敬先郭建强李巍
Owner NEW HOPE LIUHE
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