Microorganism supported palladium-gold bimetallic nano-catalyst, and preparation method and application thereof
A bimetallic nano, supported palladium technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of complex operation, instability, use of electrocatalysts, etc., and achieve a simple preparation process and environmental protection. Friendly, low-cost effects
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Embodiment 1
[0041] The present embodiment provides a kind of microorganism-loaded palladium-gold bimetallic nanocatalyst, and its preparation method is:
[0042] 1. Preparation of bacterial solution:
[0043] 1) Prepare 1L of LB medium, the main components are 10g of tryptone, 5g of yeast extract powder, 10g of NaCl, the pH value is 7.00±0.02, and it is used after high-temperature sterilization;
[0044] Prepare 2L of PBS buffer solution, the main component is Na 2 HPO 4 12H 2 O 23.1g, NaH 2 PO 4 2H 2 O5.44g, KCl0.26g, NH 4 Cl 0.62g, ready for use after high-temperature sterilization;
[0045] 2) Under aerobic conditions, inoculate the strain of Shewanella oneidensis MR-1 into the sterilized LB medium with 1% inoculum, cultivate it at 35°C and 140rpm for 12h, and then Centrifuge at 6000 rpm for 10 minutes, resuspend and wash with PBS buffer, repeat centrifugation 3 times, and collect the activated cultured bacterial liquid. Adjust the absorbance value of the bacterial suspension ...
Embodiment 2～18
[0049] Embodiment 2~18 provides the microorganism-supported palladium-gold bimetallic nanocatalyst prepared under different conditions, in its preparation method, the preparation of bacterial liquid is the same as embodiment 1, the mass ratio of palladium and gold and the time of reduction reaction are as follows Described in table 1, other parameters are with embodiment 1.
[0050] The mass ratio of table 1 embodiment 2~18 palladium and gold and the time of reduction reaction
[0051]
[0052]
[0053] test:
[0054] Above each embodiment is in the process of supporting palladium-gold bimetallic, the color of solution can change, and the SEM image of embodiment 1~15 gained microorganism-loaded palladium-gold bimetallic nanocatalysts is as follows figure 1 shown. The superscripts of Pd and Au in the figure are used to indicate the mass ratio of palladium and gold, such as bio-Pd 9 Au 1 Indicates that the mass ratio of palladium and gold is 9:1, and the others are sim...
Embodiment 19
[0060] This embodiment provides the application of the microorganism-supported palladium-gold bimetallic nanocatalysts obtained in Examples 1 to 5 in the preparation of an electrocatalytic system:
[0061] Prepare sodium tetrachloropalladate aqueous solution and sodium tetrachloroaurate solution of gradient concentration respectively, draw palladium standard curve and gold standard curve respectively by flame atomic absorption spectrometry (AAS), be used for measuring the microorganism load prepared in embodiment 1~5 The total content of palladium and gold in the type palladium-gold bimetallic nanocatalyst.
[0062] The microorganism-loaded palladium-gold bimetallic nanocatalysts prepared in the above examples 1 to 5 were respectively treated with aqua regia (concentrated HCl:concentrated HNO 3 Volume ratio 3:1) after dissolution, measure the total content of palladium and gold, load it on a glassy carbon electrode, and prepare palladium-gold loading PdAu=50μg / cm 2 glassy car...
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