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Bionic glycopolypeptide hydrogel as well as preparation method and application thereof

A technology of biomimetic sugar and hydrogel, which is applied in the fields of medical formula, medical science, bandages, etc., can solve the problems of poor tissue adhesion and biocompatibility, long wound healing time, and long hemostasis time, etc. Capacitance, rapid hemostasis and healing, and the effect of promoting healing

Active Publication Date: 2021-08-06
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the deficiencies in the prior art, the main purpose of the present invention is to provide a biomimetic glycopolypeptide hydrogel, which can realize the dual biological effects of rapid hemostasis and promotion of healing, and solve the problem of tissue adhesion and Problems such as poor biocompatibility, long hemostasis time, low hemostasis rate and long wound healing time

Method used

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  • Bionic glycopolypeptide hydrogel as well as preparation method and application thereof
  • Bionic glycopolypeptide hydrogel as well as preparation method and application thereof
  • Bionic glycopolypeptide hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Preparation of biomimetic glycopolypeptide hydrogel

[0046] (1) 1.28g APLys was dissolved in 20mL DMSO, then gluconolactone (306mg or 712mg) and 1.4mL triethylamine were added, and reacted at 50°C for 48h. The reaction solution was transferred into a dialysis bag (molecular weight cut off: 3500), dialyzed in deionized water for 48 hours, and freeze-dried to obtain glycopolypeptide P80G20 or P60G40 with a yield of 82%.

[0047](2) 183mg 3,4-dihydroxyphenylpropionic acid (DA) was dissolved in 5mL DMSO / N,N-dimethylformamide (v:v=3:1), then 232mg EDC and 139mg NHS were added, Stirred in an ice-water bath for 6h, then gradually warmed to room temperature. 10 mL of APL (0.64 g) or P80G20 (0.82 g) or P60G40 (1 g) in DMSO and 284 μL of triethylamine were added to the DA solution and reacted at room temperature for 48 h. The reaction solution was poured into a dialysis bag (MWCO: 3500), dialyzed in deionized water for 24 hours, and freeze-dried to obtain biomimet...

Embodiment 2

[0051] Example 2 Preparation of biomimetic glycopolypeptide hydrogels (Gel-7 and Gel-8)

[0052] (1) The biomimetic glycopolypeptide P420G40D20 was synthesized according to steps (1) and (2) of Example 1, and the yield was 87%.

[0053] (2) According to step (3) of Example 1, a biomimetic glycopolypeptide hydrogel Gel-7 with a coordination bond of P420G40D20 was prepared, and the polymer concentration was 4%.

[0054] (3) According to step (5) of Example 1, a covalently bonded biomimetic glycopolypeptide hydrogel Gel-8 of P420G40D20 was prepared, and the polymer concentration was 3%.

[0055] Example 2 is different from the weight average molecular weight of APLys in Example 1, the weight average molecular weight of APLys in Example 1 is 12000Da, and the weight average molecular weight of APLys in Example 2 is 56000Da.

Embodiment 3

[0056] Embodiment 3 hemolysis rate

[0057] Immerse 100 μL of biomimetic glycopolypeptide hydrogel into 500 μL of rabbit blood containing sodium citrate anticoagulant, and shake in a shaker at 37°C for 3 hours. PBS and 1% Triton X-100 were used as negative and positive controls, respectively. The blood was centrifuged for 10 min (4000 rpm), then 200 μL of the supernatant was absorbed and added to 5 mL of deionized water, and the absorbance at 540 nm was measured using a UV–Vis spectrometer. The hemolysis rate was calculated according to the formula: hemolysis rate (%)=(A sample-A negative) / (A positive-A negative)×100%, where A represents the absorbance value at 540nm. Each group of experiments was repeated 5 times, and the results were as follows: figure 1 shown.

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Abstract

The invention belongs to the field of biomedical materials, and particularly relates to bionic glycopolypeptide hydrogel as well as a preparation method and application thereof. The bionic glycopolypeptide hydrogel takes polylysine as a skeleton macromolecule, catechol groups and oligosaccharide are modified in a polymer molecular chain to obtain bionic glycopolypeptide, and under the action of dynamic crosslinking of coordinate bonds or crosslinking of covalent bonds, the coordinate bond bionic glycopolypeptide hydrogel or the covalent bond bionic glycopolypeptide hydrogel is prepared. The hydrogel not only has an extremely low hemolysis rate and excellent biocompatibility, but also realizes the dual effects of quickly stopping bleeding and promoting wound healing, and has clinical application potential in the aspects of wound bleeding stopping and tissue healing.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a biomimetic glycopolypeptide hydrogel and a preparation method and application thereof. Background technique [0002] Every year, more than 5.8 million people die from severe trauma worldwide, and about 40% of the deaths are caused by massive blood loss and its complications. Polymer hydrogel has a microstructure similar to biological tissue and good biocompatibility, and has the biological functions of hemostasis, preventing tissue inflammation, promoting tissue remodeling and accelerating wound healing. At present, important varieties such as natural polysaccharides, natural proteins, and polyethylene glycol hydrogels have been developed, but they still have poor tissue adhesion and biocompatibility, long hemostasis time, low hemostasis rate, and long wound healing time And other issues. Chinese patent CN110448721B discloses an antibacterial, adhesive, conduct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/48C08J3/075A61L26/00C08L77/04
CPCC08G69/48C08J3/075A61L26/0047A61L26/008A61L26/0066A61L26/0061A61L2300/412A61L2400/04C08J2377/04C08L77/04
Inventor 滕林董常明
Owner SHANGHAI JIAO TONG UNIV
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