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Preparation method of chondroitin sulfate

A technology of chondroitin sulfate and cartilage, applied in the biological field, can solve problems such as difficult control of process parameters, long enzymatic hydrolysis time, incomplete enzymatic hydrolysis, etc., to reduce production costs and sewage treatment costs, simple and easy to obtain, and increase added value Effect

Active Publication Date: 2021-08-10
山东广昊生物制品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of the present invention focuses on overcoming the defects of incomplete enzymolysis, long enzymolysis time, difficult control of process parameters, high lye concentration affecting the purity of chondroitin sulfate and collagen peptide in the existing process, combined with ultrafiltration and nanofiltration Separation technology purifies products, reduces environmental pollution and production costs, improves the comprehensive utilization value of poultry and livestock processing waste, realizes green and economical preparation, and is suitable for large-scale industrial production

Method used

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  • Preparation method of chondroitin sulfate
  • Preparation method of chondroitin sulfate

Examples

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Effect test

Embodiment 1

[0042] Weigh 500 g of cartilage and 500 g of trachea, and wash them twice with drinking water. Put it into a cooking pot, add distilled water to immerse, heat up to 100°C, and cook for 2 hours. After cooling down to 45°C, a small amount of sodium hydroxide was added to adjust the pH of the feed solution to 8.50.

[0043] Add 35 g of porcine pancreas and 5 g of lipase to the cooking liquid, and slowly stir with a stirring paddle, keep the temperature at 45-50°C, and react for 4 h.

[0044] In the above feed solution, a mixed enzyme containing neutral protease and collagenase was added according to the ratio of 0.20% of the raw material mass (the mass ratio of the two in the mixed enzyme was 2:1), and the temperature was maintained at 45 ° C for 2.5 h. (The pH is stable after the primary enzymatic hydrolysis, which is suitable for the secondary enzymatic hydrolysis reaction, and there is no need to add additional acid and alkali to adjust the pH). The filter residue was washed...

Embodiment 2

[0053] Weigh 250 g of cartilage and 750 g of trachea, and wash them twice with drinking water. Put it into a cooking pot, add distilled water to immerse, heat up to 100°C, and cook for 2 hours. After cooling down to 50°C, a small amount of sodium hydroxide was added to adjust the pH of the feed solution to 9.00.

[0054] 30 g of porcine pancreas and 10 g of lipase were added to the cooking liquid, supplemented by low-speed stirring with a stirring paddle, and the temperature was kept at 50°C for 2.0 h of reaction.

[0055] In the above feed solution, a mixed enzyme containing collagenase and flavor protease was added according to the proportion of 0.40% of the raw material mass (the mass ratio of the two in the mixed enzyme was 5:3), and the temperature was maintained at 50°C for 2.0 h. (The pH is stable after the primary enzymatic hydrolysis, which is suitable for the secondary enzymatic hydrolysis reaction, and there is no need to add additional acid and alkali to adjust th...

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Abstract

The invention discloses a preparation method of chondroitin sulfate, and belongs to the technical field of biology. According to the invention, cartilage and / or trachea from livestock and poultry are used as raw materials, and a modern biological enzymolysis technology and a separation and extraction technology are integrated to prepare high-purity chondroitin sulfate and protein peptide. The method comprises the following steps: cooking a mixture of cartilage and / or trachea in a reaction tank, adding sodium hydroxide to adjust the pH value to 8.0-9.5, adding trypsin and lipase to perform primary enzymolysis, and preliminarily removing impurities; adding compound protease for secondary enzymolysis, and hydrolyzing protein specifically; filtering the enzymatic hydrolysate, and carrying out ultrafiltration, concentration and drying on the supernate to obtain the high-purity chondroitin sulfate; and carrying out nanofiltration desalination, concentration and drying on the ultrafiltration permeate to obtain the collagen oligopeptide. The high-efficiency separation preparation of chondroitin sulfate and protein peptide is realized by utilizing continuous secondary enzymolysis and membrane separation technologies, the process is simple and easy to operate, the production period is short, the product purity is high, and the method is suitable for factory production and application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting chondroitin sulfate in animal cartilage and trachea. Background technique [0002] Chondroitin sulfate (CS) is an acidic mucopolysaccharide whose basic units are D-glucuronic acid and N-acetyl-D-galactosamine sulfate. Chondroitin sulfate generally contains 50-70 disaccharide basic structures with a molecular weight of about 10-50 kDa. Chondroitin sulfate has different physiological activities and can be widely used in medical, pharmaceutical, food, cosmetic and other industries. Clinically, chondroitin sulfate can be used to treat diseases such as neuralgia, arthritis, and promote ulcer healing due to its good anti-inflammatory properties. In the food industry, it can be used as a dietary supplement. [0003] At present, a lot of researches have been done on the extraction method of chondroitin sulfate in animal cartilage such as fish, pig, cattle and sheep ...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0069C08B37/0003Y02P20/10
Inventor 赵黎明刘艳琼李洁张照照张兴兴
Owner 山东广昊生物制品有限公司
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