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Gosling plague virus vaccine strain, vaccine and egg yolk antibody based on vaccine

A technology of gosling plague virus and vaccine strain, applied in the direction of virus/bacteriophage, virus antigen components, anti-viral immunoglobulin, etc., can solve the problems that restrict the healthy development of the duck industry and the enhancement of virulence, and reduce the risk of pollution , good immunogenicity, good safety effect

Active Publication Date: 2021-09-07
LIAONING YIKANG BIOLOGICAL CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Epidemiological studies have found that the virulence of some strains that are prevalent in the Pearl River Delta and its surrounding areas has increased, which restricts the healthy development of the domestic duck industry

Method used

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  • Gosling plague virus vaccine strain, vaccine and egg yolk antibody based on vaccine
  • Gosling plague virus vaccine strain, vaccine and egg yolk antibody based on vaccine
  • Gosling plague virus vaccine strain, vaccine and egg yolk antibody based on vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1. Acquisition of Gosling Plague Vaccine Strains

[0099] 1. Cases

[0100] A goose flock in a goose farm in Shandong Province was ill. The breed of goose was Landrace goose. The affected goose was 15 days old. The visible symptoms of different goslings were: diarrhea, mucus in the mouth, decreased feed intake, etc. The autopsy found ten The duodenum was swollen and congested, bleeding, covered with a large amount of light yellow mucus on the surface, hepatomegaly, congested and bleeding, fragile in texture, and the gallbladder was swollen. The clinical diagnosis was suspected gosling plague.

[0101] 2. Pathogen isolation

[0102] Rinse the diseased foie gras tissue with sterilized normal saline for 3 times, add the liver tissue into sterilized cold normal saline according to the ratio of 1g tissue: 10ml normal saline, put it into a grinder and grind it at 10000~12000r / min at 4℃ After centrifugation for 30 minutes, the supernatant was filtered through a 0.22 ...

Embodiment 2

[0124] Embodiment 2. the cultivation of gosling plague virus

[0125] Preparation and inspection of seed batches: Dilute goose plague virus seed 10 times with normal saline, and inoculate 50 12-day-old susceptible goose embryos in the allantoic cavity, 0.3ml per embryo. Incubate at 37°C without turning the eggs. The goose embryos that died 72 to 168 hours after inoculation and had obvious lesion scars were harvested respectively and put in sterilized containers. Mix the goose embryo liquid that has been tested for sterility and the death of goose embryos shows typical lesions, add a protective agent, quantitatively dispense and freeze-dry, 2.0ml / bottle, indicate the date of freeze-drying, the generation number of the virus, etc., and store at -40°C. The susceptible goose embryos were inoculated according to the above method, passed down continuously, and the E28, E32, and E36 generations were freeze-dried. After lyophilization, the virulence, safety, specificity, immunogenic...

Embodiment 3

[0138] Embodiment 3. the preparation of gosling plague virus inactivated vaccine

[0139] 1. Antigen Preparation

[0140]The antigen of the present embodiment uses goose plague virus JN18 strain E28 generation, E30 generation and the allantoic fluid of E32 generation virus goose embryo culture, the virus content is 10 respectively after testing. 5.50 ELD 50 / 0.3ml, 10 5.33 ELD 50 / 0.3ml and 10 5.25 ELD 50 / 0.3ml. No bacteria, mold, mycoplasma and exogenous virus contamination have been tested. The three batches of viruses were independently carried out as follows.

[0141] 2. Antigen inactivation and concentration

[0142] Qualified antigens were added to formaldehyde solution at a final concentration of 0.15%, inactivated at 35°C for 24 hours, centrifuged at 3000r / min for 10 minutes, and the precipitate was discarded. The supernatant was concentrated 10 times through an ultrafiltration system with a molecular weight cut-off of 30KD.

[0143] Inoculate five 8-day-old...

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Abstract

The invention discloses a gosling plague virus vaccine strain. The biological preservation number of the gosling plague virus vaccine strain is CGMCC No.22384, and the gosling plague virus vaccine strain is good in immunogenicity and good in safety. The invention further discloses an inactivated vaccine composition taking the vaccine strain as an immunogen and a preparation method of the inactivated vaccine composition. The vaccine composition is good in specificity, safe and effective. The invention further discloses an egg yolk antibody for treating, preventing, slowing down or controlling gosling plague and a preparation method of the egg yolk antibody. The vaccine composition is inoculated to poultry bodies to obtain hyper-immune poultry eggs, and the egg yolk antibody is extracted from the hyper-immune poultry eggs to prepare the egg yolk antibody. The egg yolk antibody is good in specificity, safe and effective.

Description

technical field [0001] The invention belongs to the field of biological products, and relates to a gosling plague virus vaccine strain, a vaccine and an egg yolk antibody based on the vaccine. Background technique [0002] Gosling plague virus (GPV) belongs to the family Parvoviridae, the subfamily Parvoviridae, and the genus Parvovirus. Virus particles are spherical or hexagonal, without envelope, icosahedral symmetry, nucleic acid is single-strand linear DNA, and the diameter of the virus is 20-22nm. The size of the genome is about 5kb, the middle of the genome is the coding region, and the two segments are hairpin structures formed by palindromic sequences. The coding region of the genome contains two main open reading frames, which mainly encode two kinds of nonstructural proteins NS1, NS2 and three kinds of structural proteins VP1, VP2, VP3. VP3 is the main structural protein, accounting for about 80% of the total protein, and is the main component of the viral capsid...

Claims

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Application Information

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IPC IPC(8): C12N7/00C07K16/08C07K16/02C07K1/36C07K1/34C07K1/30A61K39/12A61K39/42A61P31/20C12R1/93
CPCC12N7/00C07K16/081C07K16/02A61K39/12A61P31/20C12N2750/14321C12N2750/14334C07K2317/11A61K2039/552A61K2039/5252A61K2039/505Y02A50/30
Inventor 舒秀伟陈生雷王革新马振宇刘艳霞孙绍元宋辉刘国英
Owner LIAONING YIKANG BIOLOGICAL CORP LTD
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