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Method and device for continuously producing ellagic acid

A technology of ellagic acid and dopamine, applied in the field of tannin degradation, can solve the problems of strict requirements for enzymatic conversion conditions, cumbersome process, difficult sewage treatment and the like

Active Publication Date: 2021-09-10
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the degradation of pomegranate peel mainly includes chemical acid hydrolysis, enzyme-catalyzed reaction degradation method and microbial fermentation degradation method. Among them, the chemical acid hydrolysis method usually uses sulfuric acid, hydrochloric acid, etc. to hydrolyze the tannin extract, and the purity of ellagic acid is 20 %-40%, with high yield and purity, but it has high requirements on the corrosion resistance of equipment, and there are certain factors such as long reaction time, severe reaction conditions, equipment corrosion, difficult sewage treatment, poor regioselectivity, etc.; enzyme-catalyzed reaction The degradation method has mild reaction conditions, high selectivity and good product stability, but there are constraints such as solvent dissolution of the substrate, solvent polarity inhibition of enzyme activity, etc., and it cannot be reused and easily inactivated; microbial fermentation degradation method prepares ellagitan Acid, the main process is divided into liquid fermentation enzyme production stage and enzymatic conversion stage, this method is cumbersome, enzymatic conversion conditions are strict, difficult to control, and difficult to separate and purify the product
In addition, none of the existing pomegranate peel degradation methods can achieve continuous production, only intermittent production

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preparation example Construction

[0027] According to the present invention, the preparation method of the dopamine coating comprises: aminating the surface of the silica gel to obtain an aminated silica gel, and modifying the aminated silica gel with a fixative solution containing dopamine. The inventors found that in this preferred embodiment, it is beneficial to improve the immobilization efficiency and stability of the dopamine coating on tannase.

[0028] According to the present invention, the temperature for modifying the aminated silica gel with a fixative solution containing dopamine is 40-60°C, specifically 40°C, 45°C, 50°C, 55°C, 60°C, or between the above two values any value in between. After the modification, the modified silica gel can be washed, for example, Tris-HCl buffer solution at a flow rate of 50-150 μL / m can be used for washing.

[0029] According to the present invention, the process of the amination treatment includes: contacting the surface of the silica gel with an amination soluti...

Embodiment 1

[0046] S1. Choose a silicone tube with a length of 0.5m and an inner diameter of 0.03cm. First, use a syringe to inject 1M sodium hydroxide solution into the silicone tube for cleaning (flow rate is 100μL / min, flow rate is 1mL), and then use a syringe to make the volume fraction 10%. 3-Aminopropyltrimethoxysilane (APTES)-ethanol solution (flow rate: 100 μL / min, flow rate: 1 mL) was injected into the silica gel tube, the temperature of the incubator was adjusted to 60 ° C, and the silica gel tube was incubated at 60 ° C for 3 h to The surface of the inner cavity of the silicone tube is aminated, and the silicone tube treated with APTES is rinsed with ethanol injected from a syringe, and then baked in a thermostat at 125°C for 1 hour to dry to obtain aminated silica gel;

[0047] S2. Adjust the temperature of the incubator to 50°C, inject 1.5mL of fixative solution (with a dopamine concentration of 1mg / ml) into the silicone tube at a flow rate of 1 μL / min at 50°C with a syringe, ...

Embodiment 2

[0051] S1. Choose a silicone tube with a length of 0.4m and an inner diameter of 0.05cm. First, use a syringe to inject 1M sodium hydroxide solution into the silicone tube for cleaning (flow rate is 100μL / min, flow rate is 1mL), and then use a syringe to inject a volume fraction of 5%. 3-aminopropyltrimethoxysilane (APTES)-ethanol solution (flow rate: 50 μL / min, flow rate: 2.6 mL) was injected into the silica gel tube, the temperature of the incubator was adjusted to 50 °C, and the silica gel tube was incubated at 50 °C for 4 h The surface of the inner cavity of the silicone tube is aminated, and the silicone tube treated with APTES is rinsed with ethanol injected into a syringe, and then dried in a thermostat at 110°C for 1.5 hours to obtain aminated silica gel;

[0052] S2. Adjust the temperature of the incubator to 40°C, inject 3.8mL of fixative solution (with a dopamine concentration of 0.5mg / mL) into the silicone tube at a flow rate of 1.5μL / min at 40°C with a syringe, and...

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Abstract

The invention relates to a tannin degradation technology, and discloses a method and device for continuously producing ellagic acid. The method for continuously producing the ellagic acid comprises the following steps of: after fixing tannase through a dopamine coating, contacting the tannase with substrate solution containing tannin. The device for continuously producing the ellagic acid comprises a silicone tube, an injector used for conveying a substrate containing the ellagic acid into the silicone tube, a constant flow pump used for driving the injector and a product collector used for collecting reaction products in the silicone tube; the dopamine coating fixed with tannase is formed on the surface of an inner cavity of the silicone tube; and one end of the silicone tube is connected with the injector, and the other end of the silicone tube is connected with the product collector. The method and the device provided by the invention can implement continuous and efficient production of the ellagic acid.

Description

technical field [0001] The invention relates to tannin degradation technology, in particular to a method and device for continuous production of ellagic acid. Background technique [0002] The plant source of ellagic acid is mostly gallnut, but gallnut is the gall of animals. Although it belongs to traditional Chinese medicine, it is limited to external use, and the flower acid produced by it cannot be used as a food additive. Pomegranate peel contains a large amount of ellagic acid precursors, such as punicalagin, ellagitannin, etc., which can be hydrolyzed to produce ellagic acid, and then realize the use of waste resources to produce and prepare ellagic acid. Therefore, exploring the green and efficient degradation pathway from pomegranate peel to ellagic acid has become a hot research field of resource utilization. [0003] At present, the degradation of pomegranate peel mainly includes chemical acid hydrolysis, enzyme-catalyzed reaction degradation method and microbial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12M1/40C12M1/24C12M1/00
CPCC12P17/181C12M21/18C12M23/06C12M23/08C12M23/16C12M23/20C12M25/10C12M29/00
Inventor 韦智匀曹家南齐雅雯焦若瑜魏一平邢璐黄和宋萍徐晴
Owner NANJING NORMAL UNIVERSITY
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