On-chip in-situ cell resuscitation and suspension culture method for space environment and micro-fluidic chip and device thereof

Abstract

The invention provides an on-chip in-situ cell resuscitation and suspension culture method for a space environment. The method comprises the following steps of: inoculating cells into a cell culture layer of an in-situ cell micro-fluidic chip in a foundation laboratory, performing cryopreservation, launching a spacecraft into an orbit, and performing resuscitation. The method comprises the following steps of: 1, assembling a chip table of the in-situ cell micro-fluidic chip, and connecting a liquid path; 2, regulating the temperature of the micro-fluidic chip, and melting the cell cryopreservation solution; and 3, performing culture medium perfusion. According to the on-chip in-situ cell resuscitation and suspension culture method disclosed by the invention, the defect that the experiment can be performed in a sterile environment in the traditional technology is overcome, the experiment can be performed in a non-sterile environment, microbial pollution is avoided, and the construction cost of the space laboratory can be reduced; and moreover, according to the suspension culture, the cell culture density can be improved, the micro-fluidic chip is small in size and mass and is suitable for performing high-density culture by utilizing limited resources of space carrying tasks, and flight carrying resources can be saved.

Description

Technical field [0001] The present invention is in the field of space medical engineering and biotechnology research, particularly, to a cell suspension culture in a space environment microfluidic chip situ cryopreservation and recovery of the application sheet on recovery methods, and the microfluidic chip, and automated cell cryopreservation and recovery apparatus in situ on the sheet based resuscitation techniques. The microfluidic chip used in the process of mounting a full-sized experiment frozen, thawed and cultured cells, the present invention can be applied to various cells. Background technique [0002] Refers to a suspension culture in a liquid medium by shaking or stirring continuously, the single cell culture and tissue culture system of small cell groups, non-dependent manner for culturing adherent cells. Adherent cells may also be achieved by a suspension cell culture microcarrier, as compared to adherent cells, cell culture suspension, passaging, medium was changed...

AI Technical Summary

Problems solved by technology

On the one hand, in some launch missions, due to the limited load resources, the cell culture environment cannot be controlled for a period of time from ground launch to on-orbit, so it has a great impact on the cell state; Cells have been in the growth phase for a while, and even using special techniques can only delay cell growth to a certain extent. Therefore, if the time between the start of the on-orbit experiment and the initial inoculation is too long, the cell activity may be greatly reduced; for experiments carried out on the space station, If all experimental payloads need to be put into orbit as soon as possible to carry out experiments, there will be more time conflicts among multiple experiments; however, the number of round-trip docking of the cargo spacecraft to the space station is limited, so if all experiments need to be carried out as soon as possible after being put into orbit, there will be As a result, a large number of experiments are piled up in the days after the docking of the space station and the cargo spacecraft, which will greatly reduce the utilization rate of the space laboratory

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