Preparation method of mussel-imitating hydrogel catalytically crosslinked by hemoglobin

Abstract

The invention is applicable to the field of biological catalysis, and provides a preparation method of a mussel-imitating hydrogel catalytically crosslinked by hemoglobin, which comprises the following steps: (1) weighing a certain amount of prepared hydrogel precursor, putting into a beaker, and adding a phosphate buffer salt solution for later use; (2) weighing a certain amount of hemoglobin, and dissolving the hemoglobin in a phosphate buffer salt solution for later use; (3) preparing a phosphate buffer salt solution of H2O2 for later use; (4) measuring the hemoglobin-containing phosphate buffer salt solution in the step (2) and adding the hemoglobin-containing phosphate buffer salt solution into the solution in the step (1); (5) measuring 50 microliters of the H2O2-containing phosphate buffer salt solution prepared in the step (3), and adding the H2O2-containing phosphate buffer salt solution into the mixed solution stirred in the step (4); and (6) continuously stirring the solution in the step (5) until gel is formed.

Description

technical field [0001] The invention belongs to the field of biocatalysis, in particular to a method for preparing mussel-like hydrogel catalyzed and cross-linked by hemoglobin. Background technique [0002] Hydrogel is a material with excellent biocompatibility. It has a three-dimensional cross-linked structure similar to the extracellular matrix in the human body. Because of its excellent physical and chemical properties such as certain strength, good bioadhesion and biocompatibility properties and biological properties. At present, the adhesive materials widely used in clinical practice mainly include synthetic materials cyanoacrylate adhesive, polyurethane, protein biomimetic adhesive materials fibrin glue, gelatin and so on. However, these materials are not ideal in terms of biocompatibility, toxicity, or adhesion strength. In recent years, mussel-inspired polyphenolic hydrogels have received increasing attention. Mussel-like polyphenol hydrogels contain a large numb...

AI Technical Summary

Problems solved by technology

However, sodium periodate (NaIO 4 ), ferric chloride (FeCl 3 ) and other chemical oxidants have certain toxicity, and these preparation methods will adversely affect the biocompatibility or strength of the gel to a certain extent

Although oxidative crosslinking using HRP does not produce toxic by-products and the resulting gel has good biocompatibility, it is still not suitable for large-scale generation of hydrogels due to its high cost.

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