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Antibacterial and sensitizing active refined product of wild jujube fruits and preparation method and application thereof

A jujube, active technology, applied in the direction of antibacterial drugs, antifungal agents, organic active ingredients, etc., can solve the problem of poor efficacy of antibiotics against drug-resistant bacteria, and achieve the effects of easy skin application, mild conditions, and convenient clinical use.

Active Publication Date: 2021-10-15
YANAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The first purpose of the present invention is to provide a kind of jujube fruit antibacterial and sensitizing active refined product, which solves the problem of poor curative effect of existing antibiotics on drug-resistant bacteria

Method used

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  • Antibacterial and sensitizing active refined product of wild jujube fruits and preparation method and application thereof
  • Antibacterial and sensitizing active refined product of wild jujube fruits and preparation method and application thereof
  • Antibacterial and sensitizing active refined product of wild jujube fruits and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0059] A preparation method of the antibacterial and sensitizing active refined product of jujube fruit, comprising the following steps:

[0060] Step 1, making spiny jujube fruit into spiny jujube powder, using chloroform to extract spiny jujube powder to obtain a chloroform extract;

[0061] Specifically, step 1.1, take mature wild jujube fruit, select fruit without mechanical damage, uniform size and color, and no pests and diseases, wash off the surface dirt, put it in an oven for drying at 45°C, pulverize it, and pass it through a 80-mesh sieve to obtain Jujube powder, 10kg in total, put into a ziplock bag and store in the refrigerator for later use.

[0062] Step 1.2, first degrease jujube powder with petroleum ether, then mix it with chloroform according to the ratio of material to liquid volume ratio of 1:3, and then place it in an ultrasonic cleaner with ultrasonic power 800W, ultrasonic temperature 15-20°C, ultrasonic 50min, After filtering, the filter residue was n...

Embodiment 1

[0097] The eluate Fr.1A, Fr.1B, Fr.1C, Fr.1D, Fr.1E, Fr.1F, Fr.1G, Fr.1H, Fr.1I, Fr.1J were mixed with ampicillin (Amp) Combined use of Pseudomonas aeruginosa for joint antibacterial test, activity detection of the above eluate;

[0098] (1) Preparation of bacterial solution: 37 DEG C, 220rpm Shake and cultivate the tested bacterial strain overnight, when the bacterial solution OD600=1.0, dilute it to 1×10 6 cfu / ml;

[0099] (2) Preparation of the separated components: After accurately weighing each eluate, dissolve it in absolute ethanol and prepare the concentrations of 2560mg / ml, 1280mg / ml, 640mg / ml, 400mg / ml, 320mg / ml ml, 200mg / ml, 160mg / ml, 100mg / ml, 80mg / ml, 50mg / ml, 40mg / ml, 20mg / ml, 1mg / ml, 0.5mg / ml and 0.25mg / ml stock solutions;

[0100] (3) Preparation of antibiotics: Dilute the Amp use medium dissolved in sterile water to a mother solution with a concentration of 4096 μg / ml, filter and sterilize for later use;

[0101] (4) Adding samples: For a single drug suscep...

Embodiment 2

[0169] Example 2 Preparation of erythromycin+Fr.B ointment and its in vivo activity detection

[0170] experiment method

[0171] (1) Preparation of erythromycin+Fr.B antibacterial ointment

[0172] Matrix preparation: Weigh 21g of white vaseline and 8g of liquid paraffin, heat in a water bath to about 60°C to completely melt the matrix and stir evenly;

[0173] Antibiotic solution preparation: weigh 1g of erythromycin and dissolve it in 1ml of absolute ethanol, prepare 2 parts and set aside;

[0174] Preparation of Fr.B solution: Weigh 0.5g Fr.B and dissolve it in 1ml of absolute ethanol, prepare 2 parts and set aside;

[0175] Ointment preparation: Remove the water bath heating device that prepared the base, and when the temperature drops to about 50°C, add the prepared erythromycin solution, Fr. The proportion is 1%, and the proportion of Fr.B is 0.5%. Stir clockwise until uniform, and at the same time, lower the temperature of the mixed system to room temperature to obt...

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Abstract

The invention discloses a wild jujube fruit antibacterial and sensitizing active refined product and a preparation method and application thereof, and the preparation method comprises the following steps: preparing wild jujube fruits into wild jujube powder, and extracting the wild jujube powder with chloroform to obtain a chloroform extract; performing silica gel column chromatography separation on the chloroform extract to obtain Fr.A; carrying out silica gel column chromatography separation on the treated Fr.A to obtain Fr.B, and carrying out distillation concentration and vacuum drying on the Fr.B to obtain the antibacterial and sensitizing active refined product Fr.B of the wild jujube fruits. The Fr.B enhances the bactericidal effect of antibiotics on drug-resistant bacteria by changing the permeability of cells, has broad-spectrum antibacterial and sensitizing activity, and can play roles in diminishing inflammation and promoting wound healing.

Description

technical field [0001] The invention belongs to the technical field of spiny jujube extracts, relates to a purified product with antibacterial and sensitizing activity of jujube fruit, and also relates to a preparation method and application of the refined product. Background technique [0002] In recent years, in the treatment of bacterial infections, the inappropriate use of antibiotics has led to the emergence of widespread drug-resistant bacteria, resulting in poor efficacy of existing antibiotics against drug-resistant bacteria. In view of the continuous emergence of bacterial drug resistance, it is urgent to find new and effective methods to solve the problem of drug resistance. Among them, plant natural compounds are an important potential source for the development of new antibacterial drugs, and many plants have been studied and can function as antibacterial sensitizers. The characteristic of this type of drug is that it does not have or only has a weak antibacteri...

Claims

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Application Information

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IPC IPC(8): A61K31/201A61K31/045A61K31/20A61K31/01A61K31/231A61K31/11A61K31/23A61K31/19A61K31/198A61K31/22A61P31/04A61P29/00A61P17/02A61K36/725A61K9/06A61P31/10A61K45/06A61K31/7036A61K31/702A61K31/165A61K31/7048A61K31/56A61K31/496
CPCA61K31/201A61K31/045A61K31/20A61K31/01A61K31/231A61K31/11A61K31/23A61K31/19A61K31/198A61K31/22A61P31/04A61P29/00A61P17/02A61K36/725A61K9/06A61K9/0014A61P31/10A61K45/06A61K31/7036A61K31/702A61K31/165A61K31/7048A61K31/56A61K31/496A61K2236/35A61K2236/55A61K2236/51A61K2300/00
Inventor 林金水张恒尹芮高倩倩杨建社成娟丽张向前王凤裴永福惠雪芳
Owner YANAN UNIV
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