Modified collagen membrane and preparation method thereof, and activated multi-alkynyl cross-linking agent for modifying collagen

A collagen film and polyalkyne-based technology, applied in the field of activated polyalkyne-based cross-linking agents, can solve the problems of long reaction time of epoxy compounds, limited use of cross-linking agents, low cross-linking efficiency, etc., and achieve good biocompatibility , Improve hydrophilic ability, good cross-linking effect

Active Publication Date: 2021-10-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common collagen cross-linking agents have many problems, such as glutaraldehyde has certain cytotoxicity, genipin will react with amino acids to change color during the cross-linking process, EDC has low cross-linking efficiency and fast degradation rate during the reaction process, Epoxy compounds have a long reaction time and some residues have certain cytotoxicity, these disadvantages limit the use of these crosslinkers

Method used

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  • Modified collagen membrane and preparation method thereof, and activated multi-alkynyl cross-linking agent for modifying collagen
  • Modified collagen membrane and preparation method thereof, and activated multi-alkynyl cross-linking agent for modifying collagen
  • Modified collagen membrane and preparation method thereof, and activated multi-alkynyl cross-linking agent for modifying collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Preparation of collagen membrane

[0052] Dissolve the collagen with 1M hydrochloric acid to prepare a 6.5mg / ml collagen solution, then pour the resulting collagen solution into a petri dish, place it on a clean bench and air-dry it to obtain a collagen film. The collagen membrane was rinsed repeatedly with ultrapure water, then soaked in normal saline, and the water was changed at intervals of 30 minutes until the pH of the soaked normal saline remained basically unchanged.

[0053] (2) Synthesis of polyalkyne crosslinker 4arm-DA-PEG

[0054] Weigh 4g of four-arm polyethylene glycol 4arm-PEG-OH (Mn=10000) and 1.12g of propiolic acid into a 150ml round bottom flask, add 60ml of dry toluene solution, and then add 0.1g of p-toluene during magnetic stirring Sulfonic acid monohydrate, heat the mixed solution to 135°C, divide the by-product water during the process of condensation and reflux, take the mixed solution for evaporating and concentrating after 48 hours of reac...

Embodiment 2

[0072] To explore the effect of polyalkyne-based crosslinker 4arm-DA-PEG on the modification of collagen membrane at different temperatures:

[0073] Weigh the 4arm-DA-PEG solid and dissolve it in a conical flask of boric acid-borax buffer (pH=8.4) to form a 4arm-DA-PEG solution with a mass fraction of 20%, adjust the solution to 8.5 with 5M NaOH, add The cut collagen membranes were placed on a temperature-controllable cradle at 4°C, 20°C, and 37°C for 7 hours to obtain a series of 4arm-DA-PEG / Coll crosslinked membranes, which were washed repeatedly with ultrapure water after the reaction. Modification of collagen membranes with polyalkyne-based crosslinkers.

[0074] Cut the sample to be tested into rectangular splines with a width of 7 mm and a length of 20 mm with a knife, fully soak in normal saline for 2 hours, and then wipe off the surface moisture with filter paper. During the experiment, the sample film should always be kept in a wet state. The tensile strength and el...

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Abstract

The invention discloses a preparation method of a modified collagen membrane, the preparation method comprises the following steps: (1) preparing a collagen solution, and air-drying to obtain a collagen membrane; (2) repeatedly washing the collagen membrane with ultrapure water, soaking the collagen membrane in normal saline, and changing water for multiple times until the pH value of the soaked normal saline is kept unchanged, so as to obtain a rehydrated and swelled collagen membrane; and (3) soaking the rehydrated and swelled collagen membrane obtained in the step (2) in a solution of an activated multi-alkynyl cross-linking agent, and carrying out a cross-linking reaction to obtain the modified cross-linked collagen membrane. The invention also discloses the collagen membrane prepared by the preparation method and the activated multi-alkynyl cross-linking agent for modifying collagen. The collagen membrane is soaked in the solution of the activated multi-alkynyl cross-linking agent, so that cross-linking can be realized, the cross-linking method is simple, and the cross-linking effect is good; through the construction of a cross-linked network, the collagen with a disordered structure has an ordered structure similar to natural collagen fibers, so that the biological activity of the collagen is ensured, and the mechanical property of the collagen is enhanced.

Description

technical field [0001] The invention relates to the field of modification of collagen materials, in particular to a modified collagen film, a preparation method thereof, and an activated polyalkyne-based crosslinking agent for modifying collagen. Background technique [0002] Collagen is the main component of the extracellular matrix. It has the ability of multi-level self-assembly. It exists in almost all tissues of animals. It is the most abundant and widely distributed natural protein in mammals. The word collagen (Collagen) can be traced back to 1865, translated as "the substance that constitutes the connective tissue of animals and can be gelled during cooking." Collagen is a natural biomacromolecule, and at least one structural domain has a triple-helix conformation composed of α-chains (ie, collagen domain). The biggest feature of collagen that distinguishes it from other proteins is that it contains a large amount of proline and glycine. In the three-dimensional st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08L89/00
CPCC08J7/12C08J2389/00
Inventor 任力李昊晨彭岳海
Owner SOUTH CHINA UNIV OF TECH
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