Method for catalyzing hydrolysis and decarboxylation of grease by using cell-like multiphase emulsion photoenzyme system

A technology of multiphase emulsion and enzyme system, which is applied in the field of cell-like multiphase emulsion photoenzyme system catalyzing oil hydrolysis and decarboxylation, and can solve the problems of limiting the immobilization of fatty acid photodecarboxylase, poor light transmittance, difficult catalysis, etc.

Active Publication Date: 2021-11-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Fatty acid photodecarboxylase is a kind of biological enzyme that only depends on photocatalysis, and the reaction process must have the presence of light, so the light transmission of the system limits its application The key factor, while the traditional immobilized carrier catalytic system has poor light transmittance, which limits the immobilization of fatty acid photodecarboxylase, so no effective immobilization method for reusing the enzyme ha

Method used

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  • Method for catalyzing hydrolysis and decarboxylation of grease by using cell-like multiphase emulsion photoenzyme system
  • Method for catalyzing hydrolysis and decarboxylation of grease by using cell-like multiphase emulsion photoenzyme system
  • Method for catalyzing hydrolysis and decarboxylation of grease by using cell-like multiphase emulsion photoenzyme system

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Take 0.05g lipase AYS and place in a transparent glass bottle, 0.24g dipotassium hydrogen phosphate, 1mL (0.975g) Tris-HCl (100mM, pH=8.5) solution, mix well, add 1.5mL (about 0.6g) large intestine Bacteria whole cells (containing CvFAP) were mixed evenly, and 0.233g of polyethylene glycol 400 and 0.05g of waste cooking oil were added, put into a interlayer beaker wrapped with an LED light device capable of producing blue light, and placed at a constant temperature of 1000rpm On the stirrer, the reaction was controlled at 30°C for 12h. After the reaction, add ethyl acetate twice the volume of the system for extraction, set 12000rpm to centrifuge for 3 minutes, and divide into three layers, which are the upper liquid layer, middle liquid layer and lower liquid layer in turn. After hydrolysis, free fatty acids and long Paraffins and unreacted esters are mainly enriched in the upper liquid layer, and the upper liquid layer product is collected as the product, and the resul...

Embodiment 2

[0053] Take 0.05g lipase AYS and place in a transparent glass bottle, 0.30g sodium sulfate, 1mL (0.975g) Tris-HCl (100mM, pH=8.5) solution, mix well, add 1.5mL (about 0.6g) whole Escherichia coli Cells (containing CvFAP) were mixed evenly, added 0.279g polyethylene glycol 600 and 0.05g camellia oil, put into a sandwich beaker wrapped with an LED light device capable of producing blue light, and placed on a constant temperature stirrer with a rotation speed of 1000rpm , Control the reaction at 30°C for 12h. After the reaction, add ethyl acetate twice the volume of the system for extraction, set 12000rpm to centrifuge for 3 minutes, and divide into three layers, which are the upper liquid layer, middle liquid layer and lower liquid layer in turn. After hydrolysis, free fatty acids and long Paraffins and unreacted esters are mainly enriched in the upper liquid layer, and the upper liquid layer product is collected as the product, and the resulting product is determined by GC ( ...

Embodiment 3

[0056] Take 0.05g lipase AYS and place in a transparent glass bottle, 0.24g dipotassium hydrogen phosphate, 1mL (0.975g) Tris-HCl (100mM, pH=8.5) solution, mix well, add 1.5mL (about 0.6g) large intestine Bacillus whole cells (containing CvFAP) were mixed evenly, added 0.285g polyethylene glycol 400, 0.05g soybean oil, put into a sandwich beaker wrapped with an LED light device capable of producing blue light, and placed in a constant temperature stirring at a speed of 1000rpm On the device, the reaction was controlled at 30°C for 12h. After the reaction, add ethyl acetate twice the volume of the system for extraction, set 12000rpm to centrifuge for 3 minutes, and divide into three layers, which are the upper liquid layer, middle liquid layer and lower liquid layer in turn. After hydrolysis, free fatty acids and long Paraffins and esters that have not participated in the reaction are mainly enriched in the upper liquid layer, and the product of the upper liquid layer is collec...

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Abstract

The invention belongs to the field of chemical production and chemical synthesis, and discloses a method for catalyzing hydrolysis and decarboxylation of grease by a cell-like multiphase emulsion photoenzyme system. The method comprises the following steps of: adding soluble salt, a polymer and/or hydrophilic alcohol, fatty acid light decarboxylase and grease into lipase liquid to prepare a cell-like multiphase emulsion system; or uniformly mixing the soluble salt, the polymer and/or the hydrophilic alcohol, the fatty acid light decarboxylase and the fatty acid to prepare the cell-like multiphase emulsion system; carrying out an enzyme catalysis reaction on the cell-like multiphase emulsion system under the condition of stirring and blue light irradiation; after the reaction is finished, adding ethyl acetate for carrying out extraction; standing or centrifuging until the product is divided into three layers; and collecting a supernatant product which is a hydrolyzed and decarboxylated product. The lipase can catalyze deep hydrolysis of glyceride, the fatty acid light decarboxylase can catalyze decarboxylation of free fatty acid, while rapid reaction and intelligent control are achieved, substrate inhibition can be relieved, and a novel multi-enzyme combined catalytic system which is efficient, controllable, easy in enzyme recovery and capable of synchronously separating products is established.

Description

technical field [0001] The invention belongs to the field of chemical production and chemical synthesis, relates to enzyme separation and application technology, in particular to a method for catalyzing oil hydrolysis and decarboxylation by a cell-like multiphase emulsion photoenzyme system. Background technique [0002] Alkanes (alkenes) are compounds that play an important role in chemical production and chemical synthesis. They are non-renewable resources and can only be obtained by exploiting oil and natural gas in the short term. As a non-renewable resource, oil reserves are very limited. The traditional method for preparing alkanes (alkenes) generally uses metal ion catalysts, under high pressure, H 2 generated under the conditions. Compared with traditional chemical catalysis, enzymatic catalysis has the advantages of low energy consumption, low pollution, mild reaction conditions, and high selectivity, but it needs to be powered by expensive NADPH. In recent years...

Claims

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Application Information

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IPC IPC(8): C12P5/02
CPCC12P5/02
Inventor 李志刚梁倩杨博陈华勇马佩瑶蔡璐遥邓佩柔
Owner SOUTH CHINA UNIV OF TECH
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