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Chaetomium subaffine, application and screening method

A screening method and technology for Chaetomium, which is applied in the field of Chaetomium close relatives, application and screening, and can solve the problems of soil microbial community destruction and unclear inhibitory effect.

Active Publication Date: 2021-11-23
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the pathogenic part of ginseng root rot is the plant tissue, and the antagonistic bacteria screened from the rhizosphere soil, due to the difference in the part of action, whether it can normally exert its anti- The inhibitory effect of root rot pathogens is not clear, and strong bacteria such as Bacillus subtilis have a broad-spectrum antibacterial effect, which seriously damages the soil microbial community. Therefore, there is an urgent need for effective biological control of perennial medicinal herb diseases. microorganisms, especially for the biocontrol of ginseng

Method used

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  • Chaetomium subaffine, application and screening method
  • Chaetomium subaffine, application and screening method
  • Chaetomium subaffine, application and screening method

Examples

Experimental program
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Effect test

Embodiment 1

[0048] This example provides a method for isolating and purifying endophytic bacteria from ginseng roots by using the tissue separation method, which specifically includes washing the collected fresh ginseng roots with water first, removing the soil from the roots, washing them twice with sterile water, and then washing them with 75% ethanol Disinfect for 1 min, disinfect with 0.1% mercuric chloride for 30 s, rinse with sterile water 3 times, then use sterilized filter paper to absorb the remaining water on the root surface, and dry in the greenhouse for later use. Cut the tissue to be separated into thin slices of 0.5mm×0.5mm with a sterile scalpel, place it on a PDA plate, and culture it in an incubator at 25°C for 6 days. Four strains of fungi were obtained, respectively named as strains 1 to 4, and respectively named as MS-01 to MS-04.

Embodiment 2

[0050] This example provides a method for screening ginseng endophytes with biocontrol capabilities by using the confrontation culture method, which specifically includes picking endophyte bacteria cakes with a diameter of 5 mm from the edge of the colonies of the 4 strains of endophytes obtained in Example 1 and inserting them On the left side of the PDA plate, insert a Fusarium solani bacteria cake of the same size at the relative position of the same diameter on the right side of the PDA plate, set only the petri dish inoculated with Fusarium solani as a control, each treatment is repeated three times, and then Cultured at 25°C for 7 days, observed the growth of the colonies, and after 7 days, measured the colony radius (Rc) and the tendency radius (Rp) of only inoculated Fusarium solani fungus by the cross method, and calculated the antibacterial rate, calculated The formula is: antibacterial rate = (Rc - Rp) / Rc × 100%.

[0051] The bacteriostatic rate of 4 strains of en...

Embodiment 3

[0057] This example provides the No. 1 strain screened in Example 2 for the evaluation of antibacterial properties, specifically including activating the No. 1 strain screened on a PDA plate, and inserting the strain into PDB medium after culturing for 7 days, at 25°C, 120r·min -1 Vibrating culture for 7 days, the cultured bacterial liquid was filtered through double-layer sterile gauze to remove mycelia, and the obtained fermentation liquid was divided into two parts, one part was sterilized with an autoclave, and the other part was not treated in any way. Mix the above sterilized and non-sterilized strain No. 1 fermentation broth with the PDA medium cooled to 45°C at a volume ratio of 1:4 to make a solid plate containing the fermentation broth of biocontrol bacteria. As a control, the Fusarium solani bacteria cake with a diameter of 5mm was inoculated in the center of the plate, cultured in an incubator at 25°C for 7 days, the growth diameter of the strain was recorded, and ...

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Abstract

The invention relates to the technical field of biological control, in particular to Chaetomium subaffine, application and a screening method. The invention provides a Chaetomium subaffine, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 15, 2021, the strain preservation number is CGMCC NO.21452, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The Chaetomium subaffine has an antagonistic effect on fusarium solani and can be used for biological prevention and control of ginseng root rot, only one microorganism is introduced, damage to soil micro-ecology is reduced to the maximum extent, soil micro-ecology balance is kept, and disease prevention and control are achieved.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a chaetomium closeus, an application and a screening method. Background technique [0002] Ginseng (Panax ginseng Meyer) is a perennial medicinal herb of Araliaceae. It is a traditional and precious Chinese medicinal material in my country. The medicinal parts of ginseng are perennial roots. severe constraints. At present, the prevention and control of ginseng diseases in production mainly relies on chemical pesticides, but the unreasonable use of pesticides will cause problems such as enhanced resistance of pathogenic bacteria, drug residues, food safety and environmental pollution. [0003] Biological control has the advantages of environmental protection, safety, no residue, and strong specificity. At present, there are many strains used for biological control of ginseng root rot, such as Zhong Jiali, etc. (Zhong Jiali, Zhang Suhong, Wei Shuqin, etc. Biological Pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02A01N63/30A01P3/00C12R1/645
CPCC12N1/14C12N1/02A01N63/30
Inventor 郑培和张晶晶张宁侯微曲正义李亚丽华霜
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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