Long-chain alkane degrading bacterium and application thereof

Abstract

The invention relates to a long-chain alkane degrading bacterium and application thereof. The long-chain alkane degrading bacterium is prepared by the following method: (1) strain enrichment; (2) separation of the long-chain alkane degrading bacterium: taking 200 microliters of enrichment-cultured solution subjected to continuous enrichment culture, carrying out gradient dilution on the enrichment-cultured solution, then, coating a separation culture medium with the diluted enrichment-cultured solution, coating three culture dishes at each concentration for parallel culture, carrying out inverted culture in a constant-temperature box at the temperature of 35 DEG C, and selecting different single colonies according to forms, sizes and colors of the colonies after the colonies grow on flat plates, wherein the dilution concentration gradient is 10<-1>, 10<-2>, 10<-3>, ..., 10<-8> and 10<-9>; and (3) purification of the long-chain alkane degrading bacterium: taking separated single colonies, and carrying out inverted culture on a separation culture medium plate in a constant-temperature box at the temperature of 35 DEG C for 7 days by adopting a streak method; and repeating the steps for three times until purified single colonies are obtained, thereby obtaining the long-chain alkane degrading bacterium. The capability of degrading long-chain alkanes is relatively high, and the degradation rate can reach 78%.

Description

Technical field: [0001] The invention relates to an industrial wastewater treatment technology, in particular to a long-chain alkane-degrading bacterium and its application. Background technique: [0002] With the development of industrial technology, the water quality components of oily wastewater, coking wastewater, oil refining wastewater, papermaking wastewater and other wastewater are complex, and there are a large number of refractory biodegradable components. Among them, long-chain alkane pollutants have poor biodegradability and are difficult to treat. Bioaugmentation technology through investment Add target pollution-degrading bacteria to accelerate the degradation rate of these organic pollutants. The water quality analysis shows that although the content of long-chain alkanes in coking wastewater is not high, it is the rate-limiting factor for water quality to reach the standard. The raw water and the effluent from the secondary sedimentation tank were analyzed b...

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Problems solved by technology

Gaschromatography-mass spectrometry (GC-MS) was used to analyze the raw water and the effluent from the secondary sedimentation tank. The results showed that the penetration rate of long-chain alkane compounds was high and the degradation rate was low. Great contribution to effluent COD, difficult to deal with

[0003] The composition of coking wastewater is complex, mainly containing toxic and harmful substances such as phenol, cyanide, benzene, ammonia nitrogen, tar and sulfide, and also contains a small amount of naphthalene, pyridine, quinoline, indole, anthracene, carbazole and some other substances in the form of salts. Inorganic, hardly biodegradable

The biodegradability of coking wastewater is poor, and it is a relatively difficult biodegradable industrial wastewater

[0004] Because coal-to-gas wastewater contains high concentrations of phenolic compounds, refractory organics, and toxic pollutants, domestic and foreign coal-to-gas wastewater treatment technologies generally have problems such as unsatisfactory water output, poor system stability, and high treatment costs.

Gas chromatography-mass spectrometry (GC-MS) was used to analyze the raw water and the effluent from the secondary sedimentation tank. The results showed that the penetration rate of long-chain alkane compounds was high and the degradation rate was low. Although the content was not high, But it is the speed-limiting factor for the water quality to meet the standard, and it contributes a lot to the COD of the effluent, so it is difficult to deal with it.

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