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Blastobotrys adeninivorans A50 strain, exopolysaccharide produced by same and application of exopolysaccharide

A technology of eating Arthrospora adenicum and Arthrosporium adenilinus, which is applied in the field of microorganisms to achieve the effect of good effect, reliable strain source and strong applicability

Active Publication Date: 2021-12-17
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, functional information about the Blastobotrys adeninivorans exopolysaccharide in Liubao tea has not been reported

Method used

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  • Blastobotrys adeninivorans A50 strain, exopolysaccharide produced by same and application of exopolysaccharide
  • Blastobotrys adeninivorans A50 strain, exopolysaccharide produced by same and application of exopolysaccharide
  • Blastobotrys adeninivorans A50 strain, exopolysaccharide produced by same and application of exopolysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation, cultivation and identification of Blastobotrys adeninivorans A50 bacterial strain

[0023] 1. Isolation and cultivation of strains

[0024] (1) Collecting colonies: Take 2g of fresh Liubao tea samples in the process of fermenting (collection place: fermented tea samples during the fermenting process of Liubao tea enterprises in Wuzhou City, Guangxi Zhuang Autonomous Region), cut them into pieces with scissors, and pour them into containers. A blue bottle with 20mL of sterile water was shaken for 30min at 80r / min to shed the microorganisms attached to the tea leaves.

[0025] (2) Colony purification: Take 1 mL of colony stock solution and dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Bacterial suspension with gradient concentration, draw 200 μL of bacterial suspension, put it into PDA, and spread it evenly with a disposable coating rod. Place in an incubator at 28°C for 2 to 3 days. After the colonies grow out of the coated petri dish, o...

Embodiment 2

[0033] Embodiment 2 Fermentation of Blastobotrys adeninivorans A50 bacterial strain

[0034] 1. Seed liquid culture

[0035] The strains were taken out from the cryopreservation tube box according to the number, placed at room temperature to dissolve, inoculated into PDA, cultured at 28°C for 24 hours, and then picked a single colony by inoculation and inserted into YEPD liquid medium (peptone 20.0g, yeast powder 10.0 g, glucose 20.0g, distilled water 1000.0mL), ferment at 28°C and 120r / min for 18-24 hours, adjust the absorbance value of the bacterial liquid at 600nm to about 1.0, which is the seed liquid.

[0036] 2. Expand training

[0037] According to the inoculation ratio of 5%, it was transferred to a 500mL Erlenmeyer flask containing 250mL PDB, and fermented for 168 hours at 28°C and 120r / min.

Embodiment 3

[0038] Example 3 Extraction, Purification and Component Determination of Exopolysaccharide EPS-A50 Produced by Blastobotrys adeninivorans A50 Strain

[0039] 1. Extraction and purification

[0040]After the fermentation, the fermentation broth was centrifuged at 6000r / min for 20min, three times the volume of absolute ethanol was added, and it was precipitated overnight in a 4°C refrigerator. % ethanol, overnight at 4°C. The precipitate was collected and allowed to dry. Add distilled water to dissolve and prepare a 1% sugar solution, and use the Sevag method to remove protein. Mix equal volumes of n-butanol and distilled water, sonicate for 10 min, and take the upper layer solution as a water-saturated n-butanol solution. Add 4 times the volume of chloroform to the above n-butanol solution to obtain Sevag reagent. Add one-third times the volume of Sevag reagent to the sugar solution, stir with a magnetic stirrer for 20 minutes, pour into a separatory funnel and rest until o...

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Abstract

The invention belongs to the technical field of microorganisms, particularly relates to a Blastobotrys adeninivorans A50 strain and exopolysaccharide produced by the same, and discloses a bile acid binding effect of the exopolysaccharide. The strain provided by the invention is separated from a Liupao tea sample in a pile fermentation process, and is identified as the Blastobotrys adeninivorans bacterium through molecular biology and morphology. The exopolysaccharide of the strain contains 78.08% of total sugar and 17.81% of uronic acid, an ultraviolet spectrum shows that the exopolysaccharide does not contain nucleic acid protein, and an infrared spectrum shows that the extracellular polysaccharide is acidic polysaccharide, is sulfated polysaccharide and simultaneously contains an alpha-pyranoid ring and a furan ring. The exopolysaccharide has a good bile acid binding effect in vitro, the half inhibitory concentration IC50 value is 64.325 mg / ml, and the exopolysaccharide has potential blood fat reducing capacity and has good application prospects in the aspects of prevention and treatment of hyperlipidemia and the like.

Description

technical field [0001] The invention belongs to the technical field of microbes, and more specifically relates to a Blastobotrys adeninivorans A50 strain capable of producing extracellular polysaccharides and having a bile acid binding function and application thereof. Background technique [0002] Hyperlipidemia refers to a disease characterized by an abnormal increase in one or more lipid components in plasma (or serum). Atherosclerosis caused by this disease is an important factor in the occurrence of cardiovascular diseases. Being converted into bile acids in the liver is the main way for cholesterol to be metabolized in the body. More than 95% of the bile acids produced by the liver will be reabsorbed by the body, and the remaining small part that is not absorbed will eventually be excreted with the feces. Bile acid binders can combine with bile acids in the small intestine to form insoluble complexes and excrete them from the body, causing cholesterol in the liver or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P19/04A23L33/125A61K31/715A61P3/06C12R1/645
CPCC12N1/16C12P19/04A23L33/125A61K31/715A61P3/06A23V2002/00A23V2200/3262A23V2200/326A23V2250/51Y02A50/30
Inventor 黄丽邱思绮滕建文夏宁韦保耀林晖翔
Owner GUANGXI UNIV
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