Synthetic method of L-galactose

A synthesis method, galactose technology, applied in biochemical equipment and methods, immobilized on/in organic carriers, enzymes, etc., can solve the problems of high price, low overall yield, long preparation route, etc. The effect of cheap raw materials, simple reaction conditions and short preparation route

Active Publication Date: 2022-01-04
SOUTH CHINA NORMAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its high price greatly limits the popularization and application (the price on the internationally renowned sugar sales company Carbosynth is: L-galactose ￥ 2,300/gram)
[0003] At present, there are many preparation methods for L-galactose, but all of them have certain defects that make it impossible for cheap and large-scale production and application
[0004] In the chemical preparation route, the synthesis of this sugar needs to undergo multi-st...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synthetic method of L-galactose
  • Synthetic method of L-galactose
  • Synthetic method of L-galactose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Using galactitol as a raw material, liquid enzymes (agMDH, ScNoxE) prepare L-galactose

[0036]

[0037] Add 27.3 grams of galactitol (150 mM) and 1.42 grams of oxidized nicotinamide adenine dinucleotide disodium salt (NAD + ,2mM), then adjust the pH value of the solution to 8.0, add crude enzyme solution (1500U agMDH, 3000U ScNoxE), and then transfer the reaction solution into a pressure-resistant reactor to maintain an oxygen pressure of 1.5 atmospheres at 30°C and slowly stir the reaction 6 Hour. After the reaction, add HCl aqueous solution to adjust the pH to 1.0 to denature, precipitate the protein and remove it by high-speed centrifugation, then adjust the pH of the solution to pH 7.0, use D201 anion exchange resin (Tianjin Yunkai Resin Technology Co., Ltd.) to remove impurities, and finally use reverse osmosis After membrane desalination, concentration and crystallization (ethanol:water, 3:1, v:v) yielded 22 g of L-galactose (colorless granular sol...

Embodiment 2

[0041] Example 2: Using galactitol as a raw material, immobilized enzymes (agMDH, ScNoxE) to prepare L-galactose

[0042] Similar to Example 1, but mixed immobilized agMDH and ScNoxE enzymes are used for catalysis instead of liquid enzymes, and the immobilized enzymes can be recycled after the reaction.

[0043] Add 18.2 grams of galactitol (100 mM) and 1.42 grams of oxidized nicotinamide adenine dinucleotide disodium salt (NAD + , 2mM), then adjust the pH value of the solution to 8.0, add immobilized mixed enzyme (5000U, agMDH, ScNoxE activity unit ratio 1:5), and then transfer the reaction solution into a pressure-resistant reactor to maintain an oxygen pressure of 2 atmospheres Slowly shake the reaction at 30°C for 12 hours. After the reaction was completed, the immobilized enzyme was recovered by filtration (the immobilized enzyme was washed three times with 50mM pH 8.0 Tris buffer and stored at 4°C for later use). Concentrate and crystallize (ethanol:water, 3:1, v:v) af...

Embodiment 3

[0045] Example 3: Using galactitol as a raw material, liquid enzymes (agMDH, TtNoxE, Catalase) prepare L-galactose

[0046]

[0047] Add 18.2 grams of galactitol (100 mM) and 1.42 grams of oxidized nicotinamide adenine dinucleotide disodium salt (NAD + ,2mM), then adjust the pH value of the solution to 7.5, add crude enzyme solution (1000U agMDH, 3000U TtNoxE, 1000U catalase), and then transfer the reaction solution into a pressure-resistant reactor to maintain an oxygen pressure of 2 atmospheres at 30°C and slowly The reaction was stirred for 8 hours. After the reaction, add HCl aqueous solution to adjust the pH to 1.0 to denature, precipitate the protein and remove it by high-speed centrifugation, then adjust the pH of the solution to pH 7.0, use D201 anion exchange resin (Tianjin Yunkai Resin Technology Co., Ltd.) to remove impurities, and finally use reverse osmosis After membrane desalination, concentration and crystallization (ethanol:water, 3:1, v:v) yielded 12.8 g ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a synthesis method of L-galactose, which comprises the following steps: taking galactitol and oxidized nicotinamide adenine dinucleotide disodium salt, mixing, adding mannitol dehydrogenase and NADH oxidase, and reacting to generate L-galactose; or taking and mixing D-galactose and reduced nicotinamide adenine dinucleotide disodium salt, adding mannitol dehydrogenase and D-galactose reductase for a reaction, and generating L-galactose. According to the method, cheap and bulk D-galactose or galactitol in the market is adopted as a raw material, and expensive L-galactose is obtained through one-step or two-step direct conversion by utilizing an enzyme catalyst. The preparation scheme has multiple industrial application advantages, the used raw materials are cheap, the preparation route is short, the reaction conditions are simple, and the waste emission is low; and the characteristics well meet the basic requirements of current green industrial production.

Description

technical field [0001] The invention belongs to the field of biological enzymatic synthesis, and in particular relates to a synthesis method of L-galactose. Background technique [0002] L-galactose (L-galactose) is a rare sugar that exists in nature, and it is the L-configuration of common D-galactose. L-galactose is found in milk and sugar beets and can also be synthesized by the human body. L-galactose is a potential oral treatment for focal and segmental glomerulosclerosis in nephrotic syndrome, and it is a structural component of several bioactive saponins (Saponin), which are widely used in food, cosmetics and the field of medicine. At the same time, L-galactose is also the basic raw material for the synthesis of various L-nucleoside antiviral drugs and the biosynthesis of vitamin C. However, its high price greatly limits the popularization and application (the price on the internationally renowned sugar sales company Carbosynth is: L-galactose ￥ 2,300 / gram). [00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/02C12N9/04C12N11/08
CPCC12P19/02C12N9/0006C12Y101/01021C12Y101/01255C12N11/089
Inventor 黄华潘思婷刘励文
Owner SOUTH CHINA NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap