Synthetic method of L-galactose

Abstract

The invention discloses a synthesis method of L-galactose, which comprises the following steps: taking galactitol and oxidized nicotinamide adenine dinucleotide disodium salt, mixing, adding mannitol dehydrogenase and NADH oxidase, and reacting to generate L-galactose; or taking and mixing D-galactose and reduced nicotinamide adenine dinucleotide disodium salt, adding mannitol dehydrogenase and D-galactose reductase for a reaction, and generating L-galactose. According to the method, cheap and bulk D-galactose or galactitol in the market is adopted as a raw material, and expensive L-galactose is obtained through one-step or two-step direct conversion by utilizing an enzyme catalyst. The preparation scheme has multiple industrial application advantages, the used raw materials are cheap, the preparation route is short, the reaction conditions are simple, and the waste emission is low; and the characteristics well meet the basic requirements of current green industrial production.

Description

technical field [0001] The invention belongs to the field of biological enzymatic synthesis, and in particular relates to a synthesis method of L-galactose. Background technique [0002] L-galactose (L-galactose) is a rare sugar that exists in nature, and it is the L-configuration of common D-galactose. L-galactose is found in milk and sugar beets and can also be synthesized by the human body. L-galactose is a potential oral treatment for focal and segmental glomerulosclerosis in nephrotic syndrome, and it is a structural component of several bioactive saponins (Saponin), which are widely used in food, cosmetics and the field of medicine. At the same time, L-galactose is also the basic raw material for the synthesis of various L-nucleoside antiviral drugs and the biosynthesis of vitamin C. However, its high price greatly limits the popularization and application (the price on the internationally renowned sugar sales company Carbosynth is: L-galactose ￥ 2,300 / gram). [00...

AI Technical Summary

Problems solved by technology

However, its high price greatly limits the popularization and application (the price on the internationally renowned sugar sales company Carbosynth is: L-galactose ￥ 2,300 / gram)

[0003] At present, there are many preparation methods for L-galactose, but all of them have certain defects that make it impossible for cheap and large-scale production and application

[0004] In the chemical preparation route, the synthesis of this sugar needs to undergo multi-step hydroxyl protection and deprotection, which not only leads to the long preparation route but also the low overall yield, and at the same time, the chiral racemization problem in the preparation process will greatly increase Purification of late-stage products

[0005] The production of L-galactose by fermentation is relatively low in product concentration and purity, resulting in too high purification costs for the final product to be effectively scaled up.

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