Kit for nucleic acid extraction

A nucleic acid extraction reagent and kit technology, applied in the biological field, can solve the problems of high nucleic acid impurity content, complex nucleic acid process, poor nucleic acid quality, etc., and achieve the effects of reducing impurity content, high purity and simple process.

Pending Publication Date: 2022-01-07
易普森生物科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, the quality of nucleic acid extracted by existing nucleic acid extraction reagents is poor, the process of extracting nucleic acid is complicated, and the content of extracted nucleic acid impurities is relatively high

Method used

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  • Kit for nucleic acid extraction
  • Kit for nucleic acid extraction
  • Kit for nucleic acid extraction

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Experimental program
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Embodiment 1

[0034] The nucleic acid extraction kit provided in this embodiment includes a nucleic acid extraction reagent, wherein the nucleic acid extraction reagent includes the following components: sulfaguanidine 30g / L, stearic acid 11g / L, borax 19g / L, boric acid 12g / L, aminotriacetic acid 1.0g / L, make up to 1L with distilled water.

[0035] The extraction kit of this embodiment also includes nano magnetic beads.

[0036] In the present embodiment, the preparation process of nucleic acid extraction reagent comprises the following steps:

[0037] Step a. Take a 2L beaker, tilt the mouth of the beaker and put it into a magnetic stirrer;

[0038] Step b, take a small amount of solvent and pour it into a beaker;

[0039] Step c, weigh the guanidinium salt solid according to the above ratio, pour it into a beaker, place it on a magnetic stirrer and start stirring;

[0040] Step d, weigh the surfactant according to the above ratio, pour it into a beaker and stir for 20-30min;

[0041] S...

Embodiment 2

[0044] The nucleic acid extraction kit provided in this embodiment includes nucleic acid extraction reagents, wherein the nucleic acid extraction reagents include the following components: guanidine nitrate 35g / L, polysorbate 12g / L, glacial acetic acid 80g / L, anhydrous sodium acetate 20g / L , ammonium citrate 1.0g / L, and deionized water to make up to 1L.

[0045] The extraction kit of this embodiment also includes nano magnetic beads.

[0046] In the present embodiment, the preparation process of nucleic acid extraction reagent comprises the following steps:

[0047] Step a. Take a 2L beaker, tilt the mouth of the beaker and put it into a magnetic stirrer;

[0048] Step b, take a small amount of solvent and pour it into a beaker;

[0049] Step c, weigh the guanidinium salt solid according to the above ratio, pour it into a beaker, place it on a magnetic stirrer and start stirring;

[0050] Step d, weigh the surfactant according to the above ratio, pour it into a beaker and s...

Embodiment 3

[0054] The nucleic acid extraction kit provided in this embodiment includes a nucleic acid extraction reagent, wherein the nucleic acid extraction reagent includes the following components: 40 g / L of guanidine hydrochloride, 14 g / L of sodium dodecylbenzenesulfonate, 2 g / L of sodium carbonate, hydrogen carbonate Sodium 3g / L, ethylenediaminetetraacetic acid 1.0g / L, ultrapure water to make up to 1L.

[0055] The extraction kit of this embodiment also includes nano magnetic beads.

[0056] In the present embodiment, the preparation process of nucleic acid extraction reagent comprises the following steps:

[0057] Step a. Take a 2L beaker, tilt the mouth of the beaker and put it into a magnetic stirrer;

[0058] Step b, take a small amount of solvent and pour it into a beaker;

[0059] Step c, weigh the guanidinium salt solid according to the above ratio, pour it into a beaker, place it on a magnetic stirrer and start stirring;

[0060] Step d, weigh the surfactant according to ...

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Abstract

The invention discloses a kit for nucleic acid extraction, the kit comprises a nucleic acid extraction component, the nucleic acid extraction component comprises guanidine salt, a surfactant, a buffer component and a chelating agent; the guanidine salt is one or more of sulfaguanidine, guanidine hydrochloride, guanidine cyanate, guanidine carbonate or guanidine nitrate; the surfactant is one or more of stearic acid, sodium dodecyl benzene sulfonate, quaternization compound, lecithin, alkyl glucoside, fatty glyceride, fatty acid sorbitan or polysorbate; and the buffer component is phosphate, Tris, carbonate or acetate. According to the nucleic acid extraction kit provided by the embodiment of the invention, after the sample is extracted by the nucleic acid extraction reagent, the impurity content can be effectively reduced, background enzymes are removed to prevent product degradation, meanwhile, a target product is protected, and the extraction efficiency is remarkably improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for nucleic acid extraction. Background technique [0002] Nucleic acid is a biological macromolecular compound synthesized by many nucleotides. It is one of the most basic substances of life. It is divided into deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). RNA can be divided into ribose according to different functions. Somatic RNA (rRNA), messenger RNA (m RNA) and transfer RNA (t RNA). Nucleic acid widely exists in all animal and plant cells, microorganisms, and nucleic acids in organisms are often combined with proteins to form nucleoproteins. Different nucleic acids have different chemical compositions and nucleotide arrangement sequences. DNA is mainly concentrated in the nucleus, mitochondria and chloroplasts, while RNA is mainly distributed in the cytoplasm. [0003] Nucleic acid extraction includes the following types: ① Total RNA extraction; ② miRNA extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1013C12Q2527/125C12Q2523/32
Inventor 李小军刘仕松吴莹莹刘科
Owner 易普森生物科技(深圳)有限公司
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