Preparation method and application of fluorescent probe for detecting bimetallic ions
A fluorescent probe and bimetallic technology, applied in the field of fluorescent probes, can solve the problems of not being universally applicable, expensive, and complicated sample pretreatment, and achieve the effects of stable structure, easy storage, and low biological toxicity
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Embodiment 1
[0046] Embodiment 1 prepares the copper of purine parent, cobalt ion fluorescent probe compound
[0047] S4 preparation of copper and cobalt ion fluorescent probe compounds based on purine parent
[0048] Formula IV intermediate (200mg, 0.497mmol) and 4-diethylaminosalicylaldehyde (144mg, 0.745 mmol) were dissolved in 20mL ethanol, under N 2 Under protection, the mixture was then stirred at reflux at 80°C for 2 hours. After the point plate (TLC) tracking reaction was completed, the reaction mass was cooled to room temperature, and the solvent was distilled off under reduced pressure. Purify the crude product by petroleum ether-ethyl acetate recrystallization, add a small amount of ethyl acetate to dissolve the crude product, add a large amount of petroleum ether until it becomes cloudy, put it in the refrigerator until the crystals are precipitated, filter it with suction, and dry it to obtain the probe The copper and cobalt ion fluorescent probe compound I based on the puri...
Embodiment 2
[0054] The performance test of embodiment 2 copper, cobalt ion fluorescent probe
[0055] 1. Fluorescent probes for copper and cobalt ions in DMSO-H 2 In the O (v / v=9:1) solution, the fluorescent probe itself and the addition of copper and cobalt ions (Cu 2+ 、Co 2+ ) after the UV absorption of the fluorescent probe
[0056] The copper, cobalt ion detection fluorescent probe that embodiment 1 is made is configured into the probe stock solution of 1mM with DMSO, and each metal ion is configured into the metal ion stock solution of 3mM with deionized water, to the blank solution DMSO-H of 3mL 2 Add 30 μL of probe stock solution and 50 μL of metal ion stock solution to O (v / v=9:1) and detect with fluorescence spectrometer and ultraviolet spectrophotometer. The test shows that the maximum excitation wavelength of the fluorescent probe is 378 nm, and The emission wavelength is 472nm;
[0057] Take two cuvettes, respectively add 3mL of blank solution MeOH-H 2 Neutralize 30 μL of...
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