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Functional vegetable seedling substrate containing biocontrol bacterium microcapsules as well as preparation method and application of functional vegetable seedling substrate

A technology of vegetable seedling substrate and bio-control bacteria, applied in the field of microorganisms, can solve the problems of poor water retention, unfavorable survival and colonization of microorganisms, etc., and achieve good adaptability and strong antibacterial activity

Active Publication Date: 2022-02-08
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the technical problem that the physical and chemical environment of the seedling-raising substrate is different from that of soil, the lightweight raw material has strong air permeability but poor water retention, which is not conducive to the survival and colonization of microorganisms, the present invention Provide a functional vegetable seedling-growing substrate containing biocontrol bacteria microcapsules and its preparation method and application. The present invention aims at cultivating seedlings of tomato, cucumber, pepper and other crops. Amritsar Streptomyces S1-12 and / or Bacillus xerophilus B2-12 with disease-promoting and growth-promoting functions make the live bacteria resistant to drying and storage, and can be released slowly during seedling cultivation, stabilizing and Prolong the functional activity to achieve the effect of preventing and controlling pests and diseases and promoting the growth of seedlings

Method used

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  • Functional vegetable seedling substrate containing biocontrol bacterium microcapsules as well as preparation method and application of functional vegetable seedling substrate
  • Functional vegetable seedling substrate containing biocontrol bacterium microcapsules as well as preparation method and application of functional vegetable seedling substrate
  • Functional vegetable seedling substrate containing biocontrol bacterium microcapsules as well as preparation method and application of functional vegetable seedling substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The isolation of embodiment 1 amritsar streptomyces S1-12

[0052] Sampling the tomato rhizosphere soil samples from a facility tomato planted in Shouguang, Shandong Province, which was more than 20 years old, diluted 10 6 Take 1mL plate coated with Gaoshi No. 1 solid medium, repeat 50 plates, pick Streptomyces colonies according to the colony shape, and transfer to streak for purification.

[0053] The 16S rDNA sequence (Genbank: OK626407) of this strain was submitted to NCBI BLAST for similarity comparison, and the similarity with the type strain 2A of Streptomyces amritsarensis was 99.86%. Combined with the new morphological features, the strain was identified as Amritsarensis Streptomyces amritsarensis, named Streptomyces amritsarensis S1-12.

Embodiment 2

[0054] The antibacterial test of embodiment 2 amritsar streptomyces S1-12

[0055] (1) Rhizoctonia solani, Fusarium oxysporum, Pythium aphanidermatum, Fusarium solani, Verticillium dahliae ), Botrytis cinerea, Phytophthoracapsici and Alternaria solani were respectively inoculated on PDA medium and activated for 2 days.

[0056] (2) Use a sterilized hole puncher with a diameter of 5 mm to get a lawn from the edge of each pathogenic fungal colony, and place it on a NA medium with a diameter of 9 cm, and pick Streptomyces amritsar S1-12 with a toothpick Take a little point on the other side of the plate (6cm from the pathogenic fungus cake), and culture it at 25°C, and repeat each treatment 3 times, with only the pathogenic bacteria as the control.

[0057] (3) When the colony of the control pathogen grows to the inoculation point of Streptomyces quickly, measure the radius of each colony, and calculate the inhibition rate, the result is as follows figure 1 , as shown in Table ...

Embodiment 3

[0060] Example 3 Preparation of Functional Vegetable Seedling Substrate Containing Streptomyces amritsar S1-12 Microcapsules

[0061] Prepare Streptomyces amritsar S1-12 microcapsules according to the following steps:

[0062] (1) Streptomyces amritsarii S1-12 was inoculated into Gao's No. 1 medium for activation;

[0063] (2) The activated Streptomyces amritsarii S1-12 was transferred to the fermentation medium, the fermentation temperature was controlled in the range of 28-30°C, 180r / min shaking culture, and the effective number of viable bacteria was 10 9 -10 12 cfu / g of fermentation broth;

[0064] (3) Fermentation liquid is mixed with the carrier of 4 times of consumption, disc granulation, and wherein carrier is the mixture of at least one in diatomite, starch, active carbon and the perlite that pulverizes;

[0065] (4) Spray the prepared particles with a protective agent, the protective agent is at least two of sodium hydroxymethyl cellulose, epoxidized starch, malto...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a functional vegetable seedling substrate containing biocontrol bacterium microcapsules and a preparation method and application of the functional vegetable seedling substrate. The preparation method comprises the following steps: (1) preparing biocontrol bacterium microcapsules: mixing fermentation liquor of biocontrol bacteria with a carrier, granulating, spraying by using a protective agent to form a protective layer, and drying the granules to obtain the biocontrol bacterium microcapsules; and (2) preparing a functional vegetable seedling culture substrate: adding the biocontrol bacterium microcapsule into a conventional seedling culture substrate. For seedling cultivation of crops such as tomatoes, cucumbers, peppers and the like, on the basis of a conventional seedling cultivation substrate, microcapsules prepared from streptomyces amlyti S1-12 and / or drought-tolerant bacillus B2-12 with broad-spectrum disease-resistant and growth-promoting functions are added, so that viable bacteria are resistant to drying and storage and can be slowly released and stabilized in the seedling cultivation process, the functional activity is prolonged, and the effects of preventing and controlling diseases and insect pests, promoting growth and strengthening seedlings are achieved.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a functional vegetable seedling-raising substrate containing biocontrol bacteria microcapsules and a preparation method and application thereof. Background technique [0002] With the rapid development of facility agriculture, industrial vegetable seedling cultivation is widely popularized. Plug seedling cultivation has been widely used in countries all over the world because of its good mechanical adaptability, convenient transportation and large seedling growth per unit area. One of the main technical forms of intensive seedling cultivation. Seedling substrate is a material that can replace soil and provide sufficient water, nutrition, suitable pH value and other good root growth environment for seedling growth and development. It can fix and support seedlings to grow upright, and provide water and nutrients necessary for seed germination and seedling growth. The composition ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/02C12N11/10C12N11/14A01N63/28A01N63/22A01P5/00A01P3/00A01G24/20A01G24/28A01G24/15A01G24/22C12R1/465C12R1/07
CPCC12N1/20C12N11/10C12N11/02C12N11/14A01N63/28A01N63/22A01G24/20A01G24/28A01G24/15A01G24/22Y02P60/21
Inventor 张广志周方园谢雪迎赵晓燕范素素周红姿张新建吴晓青
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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