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Copper oxide nanorod-heme functionalized graphene as well as preparation method and application thereof

A copper oxide nanorod, heme technology is applied in the direction of analysis by chemical reaction of materials, material analysis by observing the influence on chemical indicators, measurement devices, etc., which can solve the problem of low catalytic performance and aggregation catalytic efficiency. , difficult to disperse and other problems, to achieve the effect of high catalytic performance, improved material dispersibility, and large specific surface area

Pending Publication Date: 2022-03-01
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Copper oxide nanomaterials (CuO) can mimic peroxidase under weakly acidic conditions, but the catalytic performance of single CuO is not high, so now the market introduces heteroatoms, vacancies or defect sites to regulate its catalytic activity
However, when CuO is transferred into solution, they become unstable and less dispersible, leading to aggregation and reduced catalytic efficiency.

Method used

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  • Copper oxide nanorod-heme functionalized graphene as well as preparation method and application thereof
  • Copper oxide nanorod-heme functionalized graphene as well as preparation method and application thereof
  • Copper oxide nanorod-heme functionalized graphene as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 prepares CuO / H-Gr, specific steps:

[0028] (1) GO was synthesized according to the Hummers method, and the prepared 5.0 mg GO was ultrasonically dispersed in 10 mL of secondary water to obtain 0.50 mg·mL -1 GO suspension;

[0029] (2) Dissolve 7.0 mg hemin in the GO suspension prepared in step (1), stir for 10 min, and add 200 μL NH 3 ·H 2 O and 80 μL N 2 h 4 ·H 2 O(NH 3 ·H 2 O and N 2 h 4 ·H 2 The mass fractions of O are 25% and 80% respectively); in a water bath at 60°C for 3.5h, let stand to cool to room temperature; centrifuge at 12000rpm for 15min; the product obtained is washed twice with secondary water, and then prepared by adding secondary water into 0.50mg·mL -1 H-Gr suspension;

[0030] (3) 200μL 0.020M Cu(NO 3 ) 2 ·3H 2 O and 100μL 2.0mg·mL -1 Add PVP to 2.0mL of the H-Gr suspension prepared in step (2), stir magnetically for 15min; under vigorous stirring, heat the mixture to 100°C, then quickly add 1.0mL 0.50M NaOH solution, c...

Embodiment 2

[0032] Example 2 The verification that CuO / H-Gr has peroxidase-like activity, specifically:

[0033] In 1590μL 0.20M HAc-NaAc buffer solution (pH 4.0), add 10μL 0.50mg·mL -1CuO / H-Gr obtained in Example 1 above; then add 200 μL 10 mM TMB and 200 μL 100 mM H 2 o 2 , produce a blue product oxTMB; measure the absorbance at λ=652nm with a UV-Vis absorption spectrophotometer.

[0034] Control experiment 1: replace CuO / H-Gr in the reaction system of Example 2 with H-Gr, and the rest of the experimental system is the same as that of Example 2.

[0035] Control Experiment 2: CuO / H-Gr in the reaction system of Example 2 was replaced by CuO, and the rest of the experimental system was the same as that of Example 2.

[0036] Such as figure 2 As shown, the absorbance of Example 2 is higher than that of Control Experiment 1 and Control Experiment 2, indicating that CuO / H-Gr has a synergistic catalytic effect.

Embodiment 3

[0037] Example 3 CuO / H-Gr acts as a peroxidase-like catalyst for H 2 o 2 The application experiment of oxidation substrate, the specific steps are as follows:

[0038] In 1590μL 0.20M HAc-NaAc buffer solution (pH 4.0), add 10μL 0.50mg·mL -1 CuO / H-Gr obtained in Example 1 above; then add 200 μL 12 mM TMB and 200 μL 120 mM H 2 o 2 , produce blue product oxTMB; observe the reaction color change.

[0039] Control Experiment 1: The TMB in the reaction system of Example 3 was replaced by o-phenylenediamine (OPD), and the rest of the experimental system was the same as that of Example 3.

[0040] Control Experiment 2: TMB in the reaction system of Example 3 was replaced by 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) (ABTS), and the rest of the experimental system was the same as that of Example 3.

[0041] Such as image 3 shown in H 2 o 2 In the presence of CuO / H-Gr, TMB, OPD and ABTS can be catalyzed to oxidize to blue, yellow and green products, respectively.

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Abstract

The invention relates to copper oxide nanorod-heme functionalized graphene (CuO / H-Gr) as well as a preparation method and application of the copper oxide nanorod-heme functionalized graphene (CuO / H-Gr). The peroxidase activity of CuO can be improved by simply doping a copper oxide nano material (CuO), so that the affinity between CuO and hydrogen peroxide (H2O2) is enhanced. According to the invention, a heme functionalized graphene nanosheet (H-Gr) is synthesized by a one-step hydrothermal method, and CuO nanorods are attached to H-Gr to form CuO / H-Gr. The peroxidase-like activity of the nano composite material is obviously higher than that of single CuO and H-Gr. In the presence of H2O2, the CuO / H-Gr can catalytically oxidize a substrate 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) to generate a blue product (oxTMB). However, when bisphenol F (BPF) exists, the peroxidase-like catalytic activity of the material is remarkably reduced, so that a colorimetric analysis method can be successfully constructed, and the BPF can be conveniently and quickly detected.

Description

technical field [0001] The invention relates to a nanocomposite material, in particular to a preparation method and application of copper oxide nanorod-heme functionalized graphene (CuO / H-Gr). Background technique [0002] Bisphenol F (BPF), as a substitute for bisphenol A (BPA), is widely found in consumer products such as beverage packaging, plastics, and food cans. Studies have shown that it is a toxic endocrine disruptor that can cause irreversible effects on human health, such as reduced fertility, ovarian cancer and testicular cancer. Therefore, simple and rapid detection of BPF is of great significance to human survival and health. Colorimetric analytical methods have attracted much attention due to their fast response, low cost, and simple mode of operation. [0003] Nanozymes overcome many defects of natural enzymes and apply them in many fields. Copper oxide nanomaterials (CuO) can mimic peroxidase under weakly acidic conditions, but the catalytic performance of...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/01
CPCG01N21/78G01N21/01G01N2021/0112
Inventor 郭玉晶李苗苗刘志广韩玉洁范丽芳
Owner SHANXI UNIV
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