Chemiluminescence immune microsphere freeze-drying preparation as well as preparation method and application thereof
A technology for chemiluminescence immunoassay and freeze-dried preparations, which is applied in the field of chemiluminescence immunoassay microsphere freeze-dried preparations and its preparation, which can solve the problems that the reagents used for luminescence immunoassay analysis cannot be stored and transported at room temperature, and achieve protection against denaturation and inactivation , Easy to use and easy to prepare
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Embodiment 1
[0035] This embodiment provides a chemiluminescent immune microsphere lyophilization preparation and kit and a preparation method and a detection method thereof, and a preparation method and a detection method thereof, and a preparation method and a detection method thereof and a preparation method thereof and a result of the preparation method and a detection method thereof and a preparation method and a result of the preparation method and detection method thereof and a preparation method thereof.
[0036] Brain natriuretic peptide, BNP is actually mainly from the ventricle, which is famous from the Japanese scholar SUDOH for 1988. BNP is mainly synthesized and secreted by ventricular muscle cells, with strong diastolic blood vessels, is a major endocrine system with excessive weight and high blood pressure, which can cause BNP release. The amino terminal brain naverptide precursor (NT-probNP) is one of the sodium sodium preopectures, which is an N-terminal fragment having no ac...
Embodiment 2
[0083] Example 2 to Example 5 The chemiluminescent immune microsphere lyophilized formulation provided by the present invention is used for the implementation of the quantitative detection of other immune detection indicators, and these detection and analysis are the principles of dual antibody sandwich, and use direct chemical luminescent light. Immunization, principles are as follows:
[0084] Incubate: antigens and magnetic microspheres in the sample, acridin-labeled antibody binding. Form a diblant sandwich immunization complex.
[0085] Cleaning: Under the application of a magnetic field, there is no binding substance to be separated from the immune complex by washing.
[0086] Excmune and reading: The immunocellularization is luminescence under the action of pre-transforming and excitation fluid, and chemiluminescence reactions were measured by relative light unit numbers.
[0087] The light-emitting signal value of the sample to be tested was calculated by calibration curve...
Embodiment 3
[0114] Example 3 is provided that chemiluminescent immunoacis lyophilization preparations and kits of thyroid (TSH) in human serum in vitro were used.
[0115] The thyroid hormone (TSH, Thyroid Stimulating Honnone) is a glycoprotein secreted by gland pites, including α and β from two subunits, wherein the beta subunit is a functional subunit. The secretion of TSH is adjusted by the regulation of thyroid hormone release hormones secreted by the hypothalamus and the feedback regulation of thyroid hormones in the blood circulation, which has biological rhythm.
[0116] TSH is a primary screen trial that evaluates thyroid function. Tiny variation of the free thyroid concentration will bring a significant adjustment to the reverse direction of the TSH concentration. Therefore, TSH detection is a very sensitive specific parameter that assists the evaluation of thyroid function, which is particularly suitable for early detection or elimination of hypothalamic-pituitary-thyroid shaft dysf...
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