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Multifunctional dynamic bioreactor for multiphase osteochondral scaffold

A bioreactor and multifunctional technology, applied in the field of biomedical engineering, can solve the problems of lack of heterogeneous osteochondral scaffold reactors, etc., and achieve the effect of promoting function initialization, promoting formation, promoting growth and expression of some genes

Pending Publication Date: 2022-03-15
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current osteochondral bioreactors are for single-phase scaffolds, and there is a lack of reactors for multi-phase osteochondral scaffolds.

Method used

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  • Multifunctional dynamic bioreactor for multiphase osteochondral scaffold
  • Multifunctional dynamic bioreactor for multiphase osteochondral scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The multiphase osteochondral scaffold was prepared by mold casting the biomaterial, which included chitosan 10% (w / v), silk fibroin 5% (w / v) and hydroxyapatite 10% (w / w); Solvent is PBS buffer solution;

[0053] The composition of the cartilage medium (upper layer) is 10% (v / v) fetal bovine serum, 100nmol / L dexamethasone, 35μg / mL vitamin C, 1mmol / L sodium pyruvate, 100mg / L penicillin, 100mg / L Streptomycin and 15mg / LBMP-7; the subchondral bone culture medium (lower layer) culture fluid composition is 10% (v / v) fetal calf serum, 100nmol / L dexamethasone, 10mmol / Lβ-sodium glycerophosphate, 0.2 mmol / L ascorbic acid, 100mg / L penicillin, 100mg / L streptomycin and 15mg / LBMP-2; all solvents are PBS buffer.

[0054] By adjusting the driving of the arc-shaped piston at the upper end of the dynamic biological culture device, the rotational shear force is loaded, and the liquid pressure sensors in the cartilage medium and subchondral bone medium are adjusted to load the multiphase o...

Embodiment 2

[0057] Cell-containing heterogeneous osteochondral scaffolds were prepared by bio-3D printing, and the solvent was PBS buffer; the material of the cartilage layer (upper layer) was 8% (w / v) chitosan+8% (w / v) collagen+5×10 6 cells / g bone marrow mesenchymal stem cells; the subchondral bone layer (lower layer) material is methacrylated gelatin (10% w / v), hyaluronic acid (10% w / v) and bone marrow mesenchymal stem cells ( 5×10 6 cells / g);

[0058] The composition of the culture solution (upper layer) of the cartilage medium is 15% (v / v) fetal bovine serum, 120nmol / L dexamethasone, 37.5μg / mL vitamin C, 2mmol / L sodium pyruvate, 100mg / L penicillin, 100mg / L streptomycin, 15mg / LTGF-β, 2ng / mL β-FGF, 6.25pg / ml bovine insulin, 6.25μg / mL transferrin, 15mg / LBMP-7, 1ng / mL cartilage angiogenesis inhibitor CAIF; cartilage The composition of the culture solution (lower layer) of the lower bone medium is 15% (v / v) fetal bovine serum, 120nmol / L dexamethasone, 15mmol / L beta-glycerophosphate sodium...

Embodiment 3

[0063] Bio-3D printing was used to prepare cell-containing heterogeneous osteochondral scaffolds, and the solvent was PBS buffer; the materials of the cartilage layer (upper layer) were: methacrylated gelatin (12% w / v), hydroxyapatite (5% w / w) and bone cells (5×10 6 cells / g); subchondral bone layer (lower layer) materials are: alginate (6% w / v), hyaluronic acid (6% w / v) and osteoblasts (5 × 10 6 cells / g);

[0064] The composition of the culture solution (upper layer) of the cartilage medium is 12% (v / v) fetal bovine serum, 120nmol / L dexamethasone, 37.5μg / mL vitamin C, 2mmol / L sodium pyruvate, 100mg / L penicillin, 100mg / L streptomycin, 15 mg / LTGF-β, 2 ng / mL β-FGF, 6.25 pg / ml bovine insulin, 6.25 μg / mL transferrin, 15 mg / LBMP-7, and 1 ng / mL cartilage angiogenesis inhibitor CAIF; cartilage The composition of the culture solution (lower layer) of the lower bone medium is 15% (v / v) fetal bovine serum, 120nmol / L dexamethasone, 15mmol / L beta-glycerophosphate sodium, 0.3mmol / L asco...

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Abstract

The invention provides a multifunctional dynamic bioreactor for a multiphase osteochondral scaffold, and belongs to the technical field of biomedical engineering. According to different structural characteristics of a cartilage layer and a subchondral bone layer, a dual-channel supply circulating system is adopted to separately inject corresponding culture solution to provide a required shearing force environment. Meanwhile, an arc-shaped piston is designed according to the parallel structure of cells on the cartilage surface layer and collagenous fibers, an arc-shaped rotatable loading mode is provided, and static pressure and shearing force can be added at the same time. The multifunctional dynamic bioreactor for the multiphase osteochondral scaffold can provide different mechanical stimuli (including intermittent / continuous shear stress and static pressure) according to the structures of different layers of osteochondral, can imitate the mechanical environment of a human body to the greatest extent, effectively promotes the formation of initial functional tissues of the osteochondral scaffold, and has a good application prospect. And a good environment is provided for the test of the osteochondral scaffold.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a multifunctional dynamic bioreactor for multi-phase osteochondral scaffolds. Background technique [0002] Osteoarthritis is a chronic progressive osteoarthritis pathologically characterized by articular cartilage degeneration, synovial inflammation, and surrounding bone changes. It is one of the common diseases in surgery. The clinical manifestations are intractable pain, movement Obstacles, etc., severe cases may even completely lose the ability to move joints. In people over 50 years old, osteoarthritis is second only to cardiovascular disease among the diseases that cause long-term disability, and it seriously threatens the health and daily life ability of middle-aged and elderly people. The self-repair ability of articular cartilage is extremely poor, and the purpose of repair cannot be achieved through tissue regeneration. Currently, using tissue engineeri...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/12C12M1/04C12M1/36C12M1/34C12N5/0775C12N5/077A61L27/02A61L27/12A61L27/18A61L27/20A61L27/22A61L27/24A61L27/38
CPCC12M21/08C12M29/04C12M29/06C12M35/04C12M37/02C12M41/40C12M41/34C12N5/0663C12N5/0654A61L27/24A61L27/20A61L27/22A61L27/18A61L27/12A61L27/025A61L27/3852C12N2513/00C12N2521/00A61L2430/06A61L2430/24
Inventor 胡庆夕刘亚魁张海光
Owner SHANGHAI UNIV
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