Method for preventing and treating aquatic plant tissue culture pollution bacteria

Abstract

The invention belongs to the technical field of aquatic plant tissue culture propagation, and particularly relates to a method for preventing and treating aquatic plant tissue culture pollution bacteria. According to the method for preventing and treating the aquatic plant tissue culture pollution bacteria, through separation and identification of dominant bacteria in the pollution bacteria and based on the variety separation and identification results of the pollution dominant bacteria and fungi in a tissue culture bottle, a prevention and treatment mode with an outstanding antibacterial effect is specifically screened out. According to the method for preventing and treating the aquatic plant tissue culture pollution bacteria, the common antibiotics kanamycin, chloramphenicol and potassium sorbate are combined for bacteriostasis, pollution of dominant bacteria and fungi in an aquatic plant tissue culture bottle can be inhibited at the same time, and the pollution problem in the aquatic plant tissue culture process is effectively prevented and solved through optimization of a tissue culture medium; according to the method, water plants can grow and differentiate smoothly, and normal parts of polluted tissue culture bottles can be transferred to the water plants, so that the water plants are propagated again smoothly and grow normally, and the effect of rescuing polluted plants in emergency is achieved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and propagation of aquatic plants, and in particular relates to a method for preventing and controlling bacteria polluted by tissue culture of aquatic plants. Background technique [0002] Plant tissue culture technology is an emerging technology developed on the basis of plant physiology in the early 20th century. It has been widely used in production and scientific research, and has played a huge role in the production of many crops. It has realized the industrialization of seedlings. And achieved huge economic and social benefits. Although the theoretical research on tissue culture has been quite profound, there are still some deficiencies in its application in production. For example, the pollution of the medium in tissue culture has always been a technical blind spot in this field, especially in the aspect of pollution prevention of aquatic plant tissue culture. Rarely reported. [0...

AI Technical Summary

Problems solved by technology

Although the theoretical research on tissue culture has been quite profound, there are still some deficiencies in its application in production. For example, the pollution of the medium in tissue culture has always been a technical blind spot in this field, especially in the aspect of pollution prevention of aquatic plant tissue culture. rarely reported

[0003] Usually, if the bacterial contamination in the tissue culture bottle is found before inoculation, it may be caused by the dirty culture bottle with high pressure and high temperature resistant bacteria that are not easy to be killed; if the bacterial contamination of the tissue culture bottle is found after inoculation , it may be caused by the plant material itself, or it may be caused by the use of inoculation tools that have not been thoroughly sterilized, the operator's irregular operation, or the inoculator failed to find the contaminated seedlings in time, due to cross-infection during the inoculation process If there is a large amount of fungal contamination in the tissue culture bottle before inoculation, in most cases it is due to the lack of sealing of the medium bottle, the filter screen of the ultra-clean workbench is not clean or the concentration of fungal spores in the environment where the medium is stored It is caused by too large; if fungal contamination around the explants is found in the tissue culture bottle after inoculation, it may be caused by the explant material carrying endophytic bacteria; if the fungus contamination is found in the tissue culture bottle is sporadic If it is dispersed in the culture medium, it can be determined that it is caused by the introduction of human factors

In the process of tissue culture of aquatic plants, due to other factors such as improper operation and incomplete sterilization, bacteria contamination of inoculated stems, segments and bud tips is easy to occur, such as figure 1 As shown, there are cyan, white, or yellow colonies with foggy, diffuse, or viscous shapes around the stem, which leads to the failure of callus to divide and differentiate normally, and the tissue culture process is stopped at In the early days, a lot of manpower, material and financial resources were wasted, and normal aquatic plant seedlings could not be obtained for later multiplication, scientific research experiments and market sales.

[0004] For the above situation, if strict experimental operation is carried out in the process of plant tissue culture before inoculation, it can have a good preventive effect on most bacterial contamination, but the knowledge and ability of the staff are relatively high, and the instruments and equipment Advanced and complete requirements are required, resulting in higher overall costs

At present, conventional plant tissue culture antibacterial methods are mostly to inhibit the growth of miscellaneous bacteria by adding penicillin, cephalosporin, rifampin and other antibiotics and pesticides such as carbendazim, mancozeb and chlorothalonil. Inconvenient to operate, and there are certain drug residues, resulting in adverse environmental pollution

However, most of the antibacterial research reports currently focus on terrestrial economic crops and horticultural plants, and there are few researches on the antibacterial effects of aquatic plants.

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