Bifidobacterium longum SYSU-02 and application thereof

A technology of SYSU-02 and Bifidobacterium longum, applied to bacteria, medical preparations containing active ingredients, fixed on/in organic carriers, etc., can solve the problems of lack of specific treatment methods and achieve good results Protective effect, improvement of stress resistance and storage stability at room temperature, effect of reducing severity

Active Publication Date: 2022-04-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its incidence rate is increasing year by year, and there is still a lack of specific treatment methods, and it has become a research hotspot in the field of digestion in recent years.

Method used

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  • Bifidobacterium longum SYSU-02 and application thereof
  • Bifidobacterium longum SYSU-02 and application thereof
  • Bifidobacterium longum SYSU-02 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Screening and Identification of Example 1 Bifidobacterium longum SYSU-02

[0046] Collect the feces of fresh 3-month-old healthy breastfeeding infants (Guangzhou infants, collect samples immediately after defecation) and dissolve them in an appropriate amount of sterile normal saline, and then dilute the samples by 10% with sterile normal saline. 5 times, then 20 μL was spread on the MRS solid medium, and cultured at 37°C under anaerobic conditions for 2-3 days.

[0047] Pick each single colony grown on the plate, re-apply on the new MRS solid medium for expansion culture, and then carry out strain preservation, and at the same time use the bacterial genome DNA extraction kit (Beijing Tiangen) to extract bacterial DNA, use 27F The 16S rDNA fragment was amplified and sequenced by PCR with / 1492R primer (Shanghai Sangong), and the sequencing result (SEQ ID NO: 1) was compared in the Genebank standard strain library, which is homologous to Bifidobacterium longum strain KCT...

Embodiment 2

[0052] Antibacterial experiment of embodiment 2 Bifidobacterium longum SYSU-02

[0053] will be about 10 5 CFU of Staphylococcus epidermidis ATCC 12228 and Pseudomonas aeruginosa ATCC 27853 were spread onto LB solid medium, and two Oxford cups were placed in the center of the plate. Take 200 μL of SYSU-02 fermentation supernatant cultured with MRS liquid medium at 37°C to the plateau stage and add it to an Oxford cup, and add 200 μL MRS liquid medium to another Oxford cup as a blank control.

[0054] After culturing at 37°C for 24 hours, the diameter of the inhibition zone was measured, and the experiment was repeated 3 times. The result is as image 3 As shown, SYSU-02 has significant antibacterial effect on Staphylococcus epidermidis ATCC 12228 and Pseudomonas aeruginosa ATCC 27853, and the diameters of the inhibition zone are 19±3mm and 30±4mm, respectively.

[0055] It can be seen that adding the above SYSU-02 fermentation supernatant to antibacterial cleaning products ...

Embodiment 3

[0056] The animal experiment of the prevention acute ulcerative colitis of embodiment 3 Bifidobacterium longum SYSU-02

[0057] (1) Test method

[0058] Twenty-five 4-week-old male C57BL / 6 mice were prepared and divided into 3 groups: blank control group (5 mice), model group (5 mice) and bacterial injection group (15 mice). Spread SYSU-02 on the MRS plate, and after anaerobic culture at 37°C, scrape about 10 9 For CFU of Bifidobacterium longum, resuspend the bacteria in 200 μL sterile saline. Among them, each mouse in the blank control group and the model group was fed with 200 μL sterile normal saline every day, and each mouse in the bacteria injection group was fed with 200 μL SYSU-02 bacterial solution every day for 30 days, and each mouse was recorded every day. mouse weight.

[0059] After 30 days, the mice in the model group and the bacteria-filled group were allowed to freely drink an aqueous solution containing 4% DSS (dextran sulfate) for 7 days to construct an ac...

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Abstract

The invention belongs to the technical field of microorganism application, particularly relates to bifidobacterium longum SYSU-02 and application thereof, and discloses the bifidobacterium longum SYSU-02 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.20651. The invention further discloses a preparation method of the bifidobacterium longum SYSU-02. An inhibition zone experiment and an MIC experiment show that the strain has an inhibition effect on pathogenic staphylococcus epidermidis and pseudomonas aeruginosa, and can be added into nasal cavity, oral cavity or skin cleaning products. Animal experiments prove that the strain can relieve the severity of acute ulcerative colitis and has a good protection effect on intestinal tracts. Meanwhile, the bifidobacterium longum-chitosan-inulin-calcium alginate microcapsule is constructed by adopting a microencapsulation technology and a freeze-drying technology, so that the stress resistance and the room-temperature storage stability of the strain are also improved on the basis of maintaining a relatively high freeze-drying survival rate; therefore, the survival rate of the bifidobacterium longum in the production, transportation and storage processes is ensured.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to Bifidobacterium longum SYSU-02 and its application. Background technique [0002] As an important digestive organ of the human body, the intestine not only decomposes and absorbs food, but also performs important immune functions. Lactobacilli and Bifidobacteria are probiotics that reside in the human gut. There are hundreds of millions of long-term colonized microorganisms in the human gastrointestinal tract. Under normal circumstances, these microorganisms form a good symbiotic relationship with the human body, which can help the human body digest food and maintain the immune barrier; Can lead to inflammatory or infectious diseases. [0003] Pseudomonas aeruginosa (Pseudomonas aeruginosa) is an opportunistic pathogen commonly found in the environment. common cause of machine-associated pneumonia. Staphylococcus epidermidis (Staphylococcus sepid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/04C12N1/04A23L33/135A61K35/745A61K9/50A61P1/04C12R1/01
CPCY02A50/30
Inventor 张革邱航刘嘉盛李依秋
Owner SUN YAT SEN UNIV
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