Process for producing collagen polypeptide by physical and biological double-cutting one-step method

A collagen polypeptide, biological technology, applied in the field of bioengineering, to achieve the effect of excellent product quality, high purity, and high product yield

Pending Publication Date: 2022-04-12
南宁市武鸣金三川农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a process for producing collagen polypeptides in one step with physical and biological double cutting, to solve the problem of how to use fish to produce high-quality collagen polypeptides, and to improve the purity and product yield

Method used

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  • Process for producing collagen polypeptide by physical and biological double-cutting one-step method

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Embodiment 1

[0045] A process for producing collagen polypeptides in one step with physical and biological double cleavage, comprising the following steps:

[0046] (1) 11kg of tilapia is put into the frozen meat grinder and twisted into meat-like raw material, then add 1.6‰ ascorbic acid solution to the meat-like raw material, and add 380kg of pure water, at a speed of 60r / min Stir for 7 minutes to obtain the anti-oxidation tilapia mixed slurry;

[0047] (2) Adding quality to the antioxidant tilapia mixed slurry obtained in step (1) is 2.5‰ cyclodextrin, stirring for 5min at a rotating speed of 100r / min to obtain a uniform mixture, and then the uniform mixture Drop into the colloid mill for grinding, the grinding temperature is 42°C, and the fineness is 200 mesh tilapia slurry;

[0048] (3) Enzymolysis: the aquatic animal slurry prepared in step (2) is lowered to room temperature, the pH value is adjusted to 5.2, and compound enzyme a of 2.5% of the substrate mass is added, and the compo...

Embodiment 2

[0056] A process for producing collagen polypeptides in one step with physical and biological double cleavage, comprising the following steps:

[0057] (1) Put 13kg of monkfish into a frozen meat grinder and twist it into meat-like raw materials, then add 1.8‰ ascorbic acid solution to the meat-like raw materials, and add 560kg of pure water, and stir at a speed of 70r / min 6min to obtain the antioxidant monkfish mixed slurry;

[0058] (2) Add cyclodextrin with a quality of 3‰ to the anti-oxidation monkfish mixed slurry obtained in step (1), and stir for 4 minutes at a rotating speed of 120r / min to obtain a uniform mixture, and then put the uniform mixture into Grinding in a colloid mill with a grinding temperature of 45°C to obtain a monkfish slurry with a fineness of 300 mesh;

[0059] (3) Enzymolysis: the aquatic animal slurry prepared in step (2) is lowered to room temperature, the pH value is adjusted to 5.6, and compound enzyme a with 2.8% of the substrate mass is added,...

Embodiment 3

[0067] A process for producing collagen polypeptides in one step with physical and biological double cleavage, comprising the following steps:

[0068] (1) Put 15kg of grass carp into a frozen meat grinder and twist it into a meat-like raw material, then add 2‰ ascorbic acid solution to the meat-like raw material, and add 720kg of pure water, and stir for 5 minutes at a speed of 80r / min , to obtain the antioxidant grass carp mixed pulp;

[0069] (2) To the antioxidant grass carp mixed slurry obtained in step (1), add cyclodextrin with a quality of 3.5‰, and stir for 3 minutes at a rotating speed of 150r / min to obtain a uniform mixture, and then put the uniform mixture into the colloid Grinding in the mill, the grinding temperature is 48°C, and the grass carp slurry with a fineness of 300 mesh is obtained;

[0070] (3) Enzymolysis: the aquatic animal slurry prepared in step (2) is lowered to room temperature, the pH value is adjusted to 6.0, and compound enzyme a with 2.9% of ...

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Abstract

The invention discloses a process for producing collagen polypeptide by using a physical and biological double-cutting one-step method, which belongs to the technical field of biological engineering and comprises the following steps of slurry stirring, slurry grinding, enzymolysis, full cutting, centrifugation, microfiltration, ultrafiltration, nanofiltration, spray drying and the like. The process disclosed by the invention has the advantages of high product yield, high purity, low solvent consumption, low cost and easiness in industrial production, and compared with the prior art, the process disclosed by the invention has the advantages of shorter process flow, shorter period, less pollution, no use of toxic reagents and no pollutant discharge, and achieves the goal of clean production.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a technique for producing collagen polypeptide by one-step method of physical and biological double cleavage. Background technique [0002] Protein is one of the essential substances for the growth and development of the human body and the maintenance of normal life activities. Modern biological metabolism studies have shown that after protein digestion, it is mainly absorbed directly in the form of polypeptides. Certain low-peptides can not only provide the nutrients needed for human growth and development, but also have the functions of preventing and treating diseases and regulating the physiological functions of the human body. Some hydrolyzed peptides have unique physiological functions that the original food protein or its constituent amino acids do not have. [0003] The preparation methods of collagen polypeptides mainly include chemical methods and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34C07K1/14
CPCY02P20/10
Inventor 田明黎涛何桂芳田锴瑢
Owner 南宁市武鸣金三川农业科技有限公司
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