Biological paper chip, high-throughput multi-joint detection microwell plate device, preparation method and kit for multi-joint detection of vagina inflammation
A paper chip and microplate technology, used in biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve problems such as reducing detection sensitivity, cumbersome sample addition process, and increasing missed detection rate. , to reduce the misjudgment rate, simplify the detection procedure, and improve the speed
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preparation example 1
[0058] Detection zone substrate layer:
[0059] Hydrogen peroxide detection substrate: weigh 0.5g oxidase, add deionized water to prepare 0.5g / L oxidase solution; weigh 5g of 4-aminoantipyrine, add deionized water to prepare 5g / L 4-aminoantipyrine; Weigh 10g of 2,4,6-tribromo-3-hydroxybenzoic acid, add deionized water to prepare 10g / L of 2,4,6-tribromo-3-hydroxybenzoic acid Formic acid; 0.5g / L of oxidase solution, 5g / L of 4-aminoantipyrine and 10g / L of 2,4,6-tribromo-3-hydroxybenzoic acid were mixed uniformly to obtain hydrogen peroxide detection substrate.
[0060] pH detection substrate: weigh 1g bromocresol green, add deionized water to prepare 1g / L bromocresol green solution;
[0061] Sialidase detection substrate: Weigh 2g of 5-bromo-4-chloro-3-indole neuraminic acid salt, add deionized water to prepare 2g / L 5-bromo-4-chloro-3-indole neuraminic acid Amino acid salt solution; Weigh 10g 2-methoxyl-4-morpholine diazobenzene zinc chloride and add deionized water to prepare...
preparation example 2
[0068] Substrate layer in positioning area: weigh 1g methyl violet, add deionized water to prepare 1g / L methyl violet solution;
Embodiment 1
[0071] A method for preparing a high-throughput multiple detection microporous device, comprising the following steps:
[0072] Using Adobe Illustrator software on the S1 computer to design the pattern of the hydrophilic area and the hydrophobic area of the bio-paper chip, the hydrophilic area includes a circular detection area with a diameter of 1.5 mm, and the distance between two adjacent detection areas is 0.5 mm, in a matrix distribution There are 9 detection areas in 3×3, and the detection area in the third row and third column is used as the positioning area;
[0073] S2 Print the pattern obtained in step S1 on an A4-size blank filter paper through a Xerox ColorQube8870 wax spray printer matrix to obtain a blank paper chip, wherein the blank filter paper is WhatmanTM No. 1 filter paper, and the length and width of each blank paper chip are 6.4×6.4mm;
[0074] S3 Place the blank paper chip obtained in step S2 in a metal bath at 100°C for 2 minutes to completely melt th...
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